A5073488504- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Helicobacter pylori-related gastroenterology studies
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Antimicrobial Resistance in Staphylococcus
- Reproductive tract infections research
- Genital Health and Disease
- Eosinophilic Esophagitis
- Urinary Tract Infections Management
- Streptococcal Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Gastroesophageal reflux and treatments
- Clostridium difficile and Clostridium perfringens research
- Syphilis Diagnosis and Treatment
- Tuberculosis Research and Epidemiology
- Pediatric Urology and Nephrology Studies
- Liver Disease Diagnosis and Treatment
- Pathogenesis and Treatment of Hiccups
- COVID-19 Clinical Research Studies
- Research Data Management Practices
- Veterinary medicine and infectious diseases
- Antibiotics Pharmacokinetics and Efficacy
- Species Distribution and Climate Change
Smolensk State Medical University
2019-2024
Objective. To review the basic principles and functionality of AMRmap online resource. Materials Methods. The platform was developed using R programming language various downloadable modules – packages. current annually updated version EUCAST clinical breakpoints applied for determination categories susceptibility to antimicrobial agents. Descriptive analysis includes calculation absolute relative frequencies, median values, confidence intervals Wilson method. Categorical variables are...
Surveillance of antimicrobial resistance (AMR) is crucial for identifying trends in and developing strategies prevention treatment infections. Globally, AMR surveillance systems differ terms organizational principles, comprehensiveness, accessibility, usability data presentation. Until recently, the on Russia were scarcely available, especially to international community, despite fact that large prospective multicenter was conducted accumulated over 20 years. We describe source data,...
Objective. To study the prevalence and mechanisms of antibiotic resistance, including carbapenemase production, in clinical isolates Klebsiella pneumoniae Escherichia coli isolated different regions Russia as part sentinel multicenter surveillance 2020–2021, to explore population structure K. impact “high-risk clones” on resistance. Materials Methods. Consecutive, non-duplicate (n = 2503) E. 2055) from various specimens (blood, cerebrospinal fluid, respiratory samples, urine, wound...
Antimicrobial resistance is a growing threat to global public health and requires closer attention from the medical community. Antibiotic monitoring systems play key role in observing antimicrobial susceptibility changes for rational prescribing therapy. Most current use data on scale; however, local evaluation more important practical application. Unique AMRcloud online platform (http://amrcloud.net) was developed process user resistance. Structural modules are modified address needs of...
Objective. To evaluate antimicrobial resistance patterns of clinical Streptococcus pneumoniae isolates from different regions Russia during 2014–2017. Materials and Methods. We included in the study 519 S. 18 Russian cities. Susceptibility testing was performed by reference broth microdilution method (ISO 20776-1:2006). results were interpreted according to EUCAST v.9.0 breakpoints. Results. Using non-meningeal interpretation criteria, 65.1% tested susceptible (S) penicillin; addition 28.
Objective. To assess the in vitro activity of ceftazidime-avibactam against clinical Enterobacterales and Pseudomonas aeruginosa isolates various regions Russia based on results local susceptibility testing by disk diffusion method. Materials Methods. Overall, 160 laboratories located 61 Russian cities participated this surveillance during 2018-2020. All consecutive each participating laboratory were included study. Ceftazidime-avibactam was done disc-diffusion method accordance with current...
Objective. To estimate prevalence and antimicrobial susceptibility of circulated S. pneumoniae serotypes in adult population different regions the Russian Federation. Materials Methods. A total 500 isolates obtained from patients with invasive pneumococcal disease (IPD), community-acquired pneumonia (CAP), sinusitis/acute otitis media (AOM) healthy carriers 29 centers were included study 01 June 2019 to 15 January 2020. Re-identification, typing using real-time PCR 27 primer pairs testing...
Objective. To analyze the features of local antibiotic resistance monitoring at hospitals in Russian Federation. Materials and Methods. The study involved a survey 305 institutions. duration data collection was 1 year (March 2020 – April 2021). responses received were analyzed using «R» programming language. Special packages used for processing calculation confidence intervals. Results assessed by descriptive analysis with absolute relative frequencies, 95% intervals according to Wilson...
Objective. To determine spectrum and prevalence of genetic determinants resistance to macrolides in Mycoplasma genitalium a Russian patient population. Materials Methods. A total 873 M. genitalium-positive samples from five geographical regions Russia were investigated over the period 2009–2019 using previously developed protocol real-time polymerase chain reaction (allows detecting any nucleotide substitutions 23S rRNA gene at positions 2058, 2059, 2611). The results confirmed Sanger...
Objective. To study the antimicrobial susceptibility profile of Moraxella catarrhalis isolates from different regions Russia in 2010–2021. Materials and Methods. The included 352 M. isolated 11 cities (Volgograd, Irkutsk, Kazan, Kirov, Moscow, Murmansk, Perm, Seversk, Togliatti, Tomsk, Ulan-Ude) Identification was done by MALDI-TOF mass spectrometry. Antimicrobial testing determined broth microdilution method accordance with ISO 20776-1:2020. Interpretation results EUCAST guidelines...
Objective. To study in vitro activity of antimicrobials against clinical isolates obtained from patients with community-acquired urinary tract infections (UTIs) various regions Russia 2023. Materials and Methods. The included 1098 the urine children adults both sexes all age groups acute exacerbation recurrent UTIs, including pregnant women asymptomatic bacteriuria. Isolates were collected 29 centers 21 cities 2023 as part a multicenter prospective epidemiological dynamics antibiotic...
Objective. To assess efficacy and safety of 14-day triple nifuratel-based therapy compared to standard in adult patients with symptomatic H. pylori infection. Materials Methods. A total 70 dyspepsia microbiologically confirmed infection were enrolled into the open-label, randomized, comparative clinical trial. The study group (n = 35) received a therapy: esomeprazole (20 mg BID), nifuratel (400 BID) amoxicillin (1000 BID). comparator conventional clarithromycinbased clarithromycin (500...
Objective. To determine in vitro activity of thiamphenicol and other clinically available antimicrobials against clinical isolates Haemophilus influenzae, Streptococcus pneumoniae pyogenes. Materials Methods. We included the study 875 from 20 Russian cities during 2018–2019. Among tested strains, 126 were H. 389 – S. pneumoniae, 360 Antimicrobial susceptibility testing was performed using broth microdilution method according to ISO 20776-1:2006. AST results interpreted EUCAST v.11.0...
Objective. To study management of patients with peptic ulcer based on outpatient card data and to evaluate changes in methods for diagnosis H. pylori treatment prescriptions compared 2004– 2005. Materials Methods. A total 100 cards aged 18 years older who came clinic visit over the period 2015–2018 were analyzed. The results this from pharmacoepidemiological «Ulcer», which was performed 2004–2005 Smolensk.
Objective. In this study, we examined the prevalence of macrolide-resistant M. genitalium in two Russian cities, Smolensk and Tula, between 2013 2017. Materials Methods. DNA’s samples were isolated from urethral cervicovaginal swabs using primary screening tested for macrolide resistance-associated mutations by real-time PCR. This technology makes it possible to identify any nucleotide substitutions 23S rRNA gene at positions 2058, 2059, 2611 melting curve analysis after amplification....
Objective. To evaluate in vitro activity of biapenem and other clinically available carbapenems against Russian clinical isolates Enterobacterales, Pseudomonas aeruginosa и Acinetobacter spp., including with acquired fermentative mechanisms resistance to β-lactams. Materials Methods. A total 3139 Enterobacterales isolates, 793 P. 634 spp. from hospitalized patients 63 hospitals 35 cities were included the study during 2018-2019. Minimal inhibitory concentrations (MIC) for antimicrobials...
Objective. To assess efficacy and safety of 14-day triple nifuratel-based therapy compared to standard in adult patients with symptomatic H. pylori infection. Materials Methods. A total 70 dyspepsia microbiologically confirmed infection were enrolled into the open-label, randomized, comparative clinical trial. The study group (n = 35) received a therapy: esomeprazole (20 mg BID), nifuratel (400 BID) amoxicillin (1000 BID). comparator conventional clarithromycinbased clarithromycin (500...
Objective. To identify risk factors for fatal outcome and COVID-19-associated liver damage in hospitalized adult patients with coronavirus infection. Materials Methods. In a retrospective cohort study, 389 cases of infection complicated by bilateral viral pneumonia were studied. Demographic characteristics, clinical features the course disease, anamnestic data, results laboratory instrumental methods examination analyzed correlated mortality. At time admission, following taken into account:...
Objective. To review the key principles and functionality of AMRexpert online platform. Materials Methods. The information part platform is comprised rules based on EUCAST recommendations various standards for interpreting results antimicrobial susceptibility testing (EUCAST, CLSI versions 2020-2022). technical was developed using C# programming language, Angular Bootstrap frameworks. AST Serratia marcescens, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Enterococcus faecium specific...
Objective. To analyse aggregated AST results for key microorganisms collected through the 2022 reports of chief specialists in clinical microbiology and antimicrobial resistance. Materials Methods. The study included an analysis interpretation criteria used laboratories evaluation reports. Data were obtained from resistance annual reporting system. Reports analyzed using EUCAST guidelines expected phenotypes susceptible phenotypes. processing realized «R» programming language. 95% CI...
Objective. To determine in vitro activity of oral III generation cephalosporin cefpodoxime against clinical isolates Haemophilus influenzae, Streptococcus pneumoniae and pyogenes isolated from patients with community-acquired respiratory tract infections different regions the Russian Federation. Materials Methods. The study included bacterial pathogens outpatients hospitalized A total 558 were study, including 184 H. 186 S. 188 pyogenes. Species identification was performed using MALDI-TOF...