A5027224393- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Photoreceptor and optogenetics research
- Light effects on plants
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- Infant Nutrition and Health
- Polysaccharides and Plant Cell Walls
- Viral-associated cancers and disorders
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Parvovirus B19 Infection Studies
- Bioeconomy and Sustainability Development
- Enzyme Catalysis and Immobilization
- Circadian rhythm and melatonin
- Probiotics and Fermented Foods
- Glycosylation and Glycoproteins Research
- Retinal Development and Disorders
East China Normal University
2019-2025
Georgia Institute of Technology
2017-2018
Jiangnan University
2013-2017
Yangtze Normal University
2017
Roswell Park Comprehensive Cancer Center
1991
Some possible ways in which replication of plasmids containing the Epstein-Barr virus (EBV) plasmid maintenance origin, oriP, might be controlled were investigated. Virtually all molecules found to replicate no more than once per cell cycle, whether was observed after stable introduction into cells by drug selection or during first few divisions introducing DNA cells. The presence excess amounts EBNA1, only viral protein needed for oriP function, did not increase number oriP-replicated...
It is widely understood that CRISPR-Cas9 technology revolutionary, with well-recognized issues including the potential for off-target edits and attendant need spatiotemporal control of editing. Here, we describe a far-red light (FRL)-activated split-Cas9 (FAST) system can robustly induce gene editing in both mammalian cells mice. Through light-emitting diode-based FRL illumination, FAST efficiently edit genes, nonhomologous end joining homology-directed repair, multiple loci human cells....
Bacteria-based therapies are powerful strategies for cancer therapy, yet their clinical application is limited by a lack of tunable genetic switches to safely regulate the local expression and release therapeutic cargoes. Rapid advances in remote-control technologies have enabled precise control biological processes time space. We developed therapeutically active engineered bacteria mediated sono-activatable integrated gene circuit based on thermosensitive transcriptional repressor TlpA39....
Abstract Gene therapies provide treatment options for many diseases, but the safe and long-term control of therapeutic transgene expression remains a primary issue clinical applications. Here, we develop muscone-induced system packaged into adeno-associated virus (AAV) vectors (AAV MUSE ) based on G protein-coupled murine olfactory receptor (MOR215-1) synthetic cAMP-responsive promoter (P CRE ). Upon exposure to trigger, muscone binds MOR215-1 activates cAMP signaling pathway initiate...
Abstract Propionic acid (PA) is an important platform chemical in the food, agriculture and pharmaceutical industries mainly biosynthesized by propionibacteria. Acid tolerance PA-producing strains crucial. In previous work, we investigated mechanism of Propionibacterium acidipropionici at microenvironmental levels analyzing physiological changes parental strain three PA-tolerant mutants obtained genome shuffling. However, molecular PA P. remained unclear. Here, performed a comparative...
Propionic acid (PA) is an important chemical building block widely used in the food, pharmaceutical, and industries. In our previous study, a shuttle vector was developed as useful tool for engineering Propionibacterium jensenii, two key enzymes-glycerol dehydrogenase malate dehydrogenase-were overexpressed to improve PA titer. Here, we aimed production further via pathway of P. jensenii. First, phosphoenolpyruvate carboxylase gene (ppc) from Klebsiella pneumoniae access one-step synthesis...
The Cre-loxP recombination system enables precise genome engineering; however, existing photoactivatable Cre tools suffer from several limitations, including low DNA efficiency, background activation, slow activation kinetics, and poor tissue penetration. Here, we present REDMAPCre, a red-light-controlled split-Cre based on the ΔPhyA/FHY1 interaction. REDMAPCre rapid (1-second illumination) achieves an 85-fold increase in efficiency. We demonstrate its efficient regulation of mammalian cells...
ABSTRACT Propionic acid (PA) and its salts are widely used in the food, pharmaceutical, chemical industries. Microbial production of PA by propionibacteria is a typical product‐inhibited process, resistance crucial improvement titers productivity. We previously identified two key elements—the arginine deaminase glutamate decarboxylase systems—that protect against stress maintaining intracellular pH homeostasis. In this study, we attempted to improve Propionibacterium jensenii ATCC 4868...
Abstract Acid stress induced by the accumulation of organic acids during fermentation propionibacteria is a severe limitation in microbial production propionic acid (PA). To enhance resistance strains, tolerance mechanisms cells must first be understood. In this study, comparative genomic and transcriptomic analyses were conducted on wild‐type acid‐tolerant Propionibacterium acidipropionici to reveal response fermentation. Combined with results previous proteomic metabolomic studies, several...
A synthetic switch shows tunable transgene expression, regulatable genome engineering, and programmable biocomputation in mammals.
We describe here a novel approach to enhance the transcription of target gene in cell-free systems by symmetrically introducing duplex aptamers upstream T7 promoter both sense and antisense strands double-stranded plasmids, which leads formation DNA bubble due none-complementary state ssDNA region harboring aptamer sequences. With presence thrombins, would be enlarged binding with thrombins. Consequently, recognition contained can more easily recognized bound RNA polymerases, separation...
Microbial catalysis has recently emerged as one of the most promising approaches in oligosaccharide synthesis. However, despite significant progress, microbial synthesis still requires much improvement efficiency and reduction process complexity. Additionally, given stunning diversity many varied applications glycans, broadening range glycans accessible via is paramount importance. Major challenges include catabolite repression high cellular energy requirement. Here we demonstrated a new...