New diketopiperazines, epicoccins E−H (1−4) and diphenylalazines A (5) B (6), have been isolated from the solid-substrate fermentation culture of Cordyceps-colonizing fungus Epicoccum nigrum. The structures 1−6 were determined primarily by NMR experiments, structure 1 was confirmed X-ray crystallography. absolute configurations 3 5 assigned using modified Mosher (3) Marfey's methods, respectively. Compounds 3−5 showed inhibitory effects on HIV-1 replication in C8166 cells.

Abstract Three new sesquiterpene acids, xylaric acids A–C ( 1 – 3 , resp.), and a tetralone (=3,4‐dihydronaphthalen‐1(2 H )‐one) derivative, 4 along with nine known compounds, acid D 5 ), hydroheptelidic 6 gliocladic 7 chlorine heptelidic 8 trichoderonic A 9 16‐( α ‐ ‐mannopyranosyloxy)isopimar‐7‐en‐19‐oic 10 ‐glucopyranosyloxy)isopimar‐7‐en‐19‐oic 11 5‐carboxymellein 12 naphthalen‐1,8‐diol 1‐ O ‐glucopyranoside 13 ) have been isolated from the solid culture of ascomycete fungus Xylaria sp....

Abstract Objective. Hybrid proton-photon radiotherapy (RT) is a cancer treatment option to broaden access proton RT. Additionally, with refined planning method, hybrid RT has the potential offer superior plan quality compared proton-only or photon-only RT, particularly in terms of target coverage and sparing organs-at-risk (OARs), when considering robustness setup range uncertainties. However, there concern regarding underestimation biological effect protons on OARs, especially those close...

Abstract Trichocladinols A–C ( 1 – 3 ), three new metabolites, and the known massarigenin A 4 have been isolated from cultures of a Cordyceps ‐colonizing ascomycete Trichocladium opacum . Their structures were elucidated by NMR spectroscopy X‐ray crystallography. The absolute configuration was assigned using modified Mosher method that determined crystallographic analysis its S )‐MTPA ester. Compounds showed modest cytotoxic effects against human tumor cell lines HeLa MCF‐7. Structurally,...

Exogenous pollution of Chinese medicinal materials by pesticide residues and heavy metal ions has attracted great attention. Relying on the rapid development nanotechnology multidisciplinary fields, fluorescent techniques have been widely applied in contaminant detection monitoring due to their advantages simple preparation, low cost, high throughput others. Most importantly, synchronous multi-targets always pursued as one major goals design probes. Herein, we firstly develop a simultaneous...

Abstract The relationship between genotype and fitness is fundamental to evolution, but quantitatively mapping genotypes has remained challenging. We propose the Phenotypic-Embedding theorem (P-E theorem) that bridges genotype-phenotype through an encoder-decoder deep learning framework. Inspired by this, we proposed a more general first principle for correlating genotype-phenotype, provides computable basis application of principle. As example P-E theorem, developed Co-attention based...

The Yellow River Source Area (YRSA) in China is an important water yield and conservation area the basin.Alterations climate landscape patterns have had a significant impact on services of YRSA.In this study, we used modified parameter module InVEST model to calculate equation order evaluate spatiotemporal changes YRSA from 2000 2020.The results show that increased at rates 14.72 mm/5a 10.03 mm/5a, respectively, 2020, both showed decreasing pattern southeast northwest.Precipitation main...

The Professional Committee of Microbiology the National Pharmacopoeia Commission organized drafting Technical Guidelines for Microbial Whole Genome Sequencing (WGS), aiming to standardize method process and technical indicators microbial WGS ensure accuracy sequencing identification. On basis Guidelines, we developed an integrated identification source tracking (MIST) system, which could meet needs contamination investigation in food drug quality control. MIST integrates three analysis...

Agaricus bisporus lectin (ABL), which is one of the antinutritional factors in A. bisporus, an important allergen and harmful to human health. Due shortcomings current detection methods, it extremely urgent establish a rapid sensitive method for ABL foods. To isolate ssDNA aptamer ABL, 13 rounds subtractive systematic evolution ligands by exponential enrichment (SELEX) selection were carried out. As result, six candidate aptamers selected further examined their binding affinity specificity...

Abstract The relationship between genotype and fitness is fundamental to evolution, but quantitatively mapping genotypes has remained challenging. We propose the Phenotypic-Embedding theorem (P-E theorem) that bridges genotype–phenotype through an encoder–decoder deep learning framework. Inspired by this, we proposed a more general first principle for correlating genotype–phenotype, P-E provides computable basis application of principle. As example theorem, developed Co-attention based...

A method for the quantitative determination of ganoderic acid was constructed using principle indirect competitive enzyme-linked immunosorbent assay (ELISA), and this used to determine contents Ganoderma lucidum samples in market. The conjugate bovine serum albumin four rounds immunization on test rabbits obtain rabbit antiganoderic antibody IgG. enzyme-labeled plate coated with ovalbumin. first stage reaction ELISA that sample competed bind antibodies. second combination goat anti-rabbit...

Etomidate is commonly used in the induction of anesthesia. We have previously confirmed that etomidate requirements are significantly reduced patients with obstructive jaundice and anesthesia during Endoscopic Retrograde Cholangiopancreatography (ERCP) results more stable hemodynamics compared to propofol. The aim present study investigate whether affects pharmacokinetics who underwent bile duct surgery.A total 18 12 non-jaundiced scheduled for surgery were enrolled study. 0.333 mg/kg was...

The presence of water in transformer oil will reduce its insulation performance, resulting significant accidents such as breakdown the transformer. traditional method measuring content has shortcomings poor real-time performance and low accuracy. This paper proposes for first time to apply principle calculable cross capacitor field measurement oil. A new sensor structure based on is designed. It uses finite element software Comsol Multiphysics simulate analyze influence structural parameters...