A5100717083- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Helicobacter pylori-related gastroenterology studies
- Enterobacteriaceae and Cronobacter Research
- Pneumonia and Respiratory Infections
- Antimicrobial Peptides and Activities
- Plant responses to water stress
- Environmental and Agricultural Sciences
- Medical Research and Treatments
- Ocular Infections and Treatments
- Flowering Plant Growth and Cultivation
- Advances in Cucurbitaceae Research
- Synthesis of β-Lactam Compounds
- Cytokine Signaling Pathways and Interactions
- bioluminescence and chemiluminescence research
- Polymer-Based Agricultural Enhancements
- Regional Economic and Spatial Analysis
- Alzheimer's disease research and treatments
Xiamen University
2019-2024
Anhui University of Traditional Chinese Medicine
2022
Shenzhen University Health Science Center
2018-2022
Zhujiang Hospital
2022
Southern Medical University
2022
Shenzhen University
2018-2021
Shenzhen Sixth People's Hospital
2018-2021
ShenZhen People’s Hospital
2021
Guangdong Medical College
2018-2020
Shanghai Medical College of Fudan University
2020
Klebsiella pneumoniae bacteremia biofilm traits and distribution characteristics have not been clarified. This study aimed to determine the prevalence of K. formation (BF) explore virulence factors associated with BF. A total 250 isolates were collected from patients in Shenzhen Shanghai, China. Virulence genes their genomes detected by PCR. The subjected multilocus sequence typing (MLST) clonal complex (CC) classification based on housekeeping genes. Biofilms crystal violet staining....
This study investigated the characteristics and mechanisms of eravacycline resistance heteroresistance in clinical Klebsiella pneumoniae isolates. A total 393 K. isolates were collected subjected to tigecycline MIC determinations using agar dilution method. Eravacycline was assessed by a population analysis profile (PAP). The expression levels efflux pumps their regulators determined quantitative reverse-transcription PCR (qRT-PCR). identified 67 eravacycline-nonsusceptible isolates; among...
Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) has been increasingly reported and is now recognized as a significant threat to public health; however, characterization of MDR-hvKP not systematically investigated. In the present study, 124 428 (28.92%) K. clinical isolates collected from January 2010 December 2016 were identified with aerobactin defined hvKP; these included 94 non-MDR-KP, 20 extended-spectrum β-lactamase-producing (ESBL-KP), 10 carbapenem-resistant (CR-KP)...
Abstract Background ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of Enterococcus faecalis remains obscure. A clpP deletion mutant (△ ) was constructed using E. OG1RF strain to clarify effect on faecalis. The global abundance proteins determined a mass spectrometer with tandem tag labeling. Results Δ showed impaired at 20 °C 45 5% NaCl 2 mM H O ....
Biofilm formation and hemolysis induced by Staphylococcus aureus are closely related to pathogenicity. However, no drugs exist inhibit biofilm or S. in clinical practice. This study found diclazuril had antibacterial action against with minimum inhibitory concentrations (MICs) at 50 μM for both methicillin-sensitive (MSSA) methicillin-resistant (MRSA). Diclazuril (at 1/4× 1/8× MICs) significantly inhibited of under static flow-based conditions also aureus. The RNA levels transcriptional...
Solithromycin, the fourth generation of ketolides, has been demonstrated potent antibacterial effect against commonly-isolated gram-positive strains. However, Staphylococcus aureus (S. aureus) strains with a higher solithromycin MIC have already emerged, mechanism which is unknown. Antimicrobial susceptibility test was performed on 266 S. aureus. The antibiotic resistance phenotype erm-positive strain determined by D-zone test. Spontaneous mutation frequency analysis to compare risk levels...
Background: Enterococcus faecalis has been commonly considered as one of the major pathogens urinary tract infection (UTI) in human host worldwide, whereas molecular characteristics E. clinical isolates from patients with UTI China remains seldomly reported. This study aimed to investigate resistance mechanism, and risk factors China. Methods: A total 115 non-duplicated were retrospectively collected a tertiary hospital their data was further analyzed. The linezolid tedizolid susceptibility...
Mortality rates for patients with Staphylococcus aureus (S. aureus) infections have improved only modestly in recent decades and S. remain a major clinical challenge This study investigated the vitro antimicrobial activity of erevacycline (erava) against isolates from China, as well heteroresistance frequency erava sequence types (STs) represented sample.A sample 328 non-duplicate isolates, including 138 methecillin-resistant (MRSA) 190 methecillin-sensitive (MSSA) were collected...
Omadacycline (Omad), a new tetracycline (Tet)-class broad-spectrum aminomethylcycline, has been reported to exhibit excellent potency against Gram-positive bacteria, including Staphylococcus aureus and Enterococci. The aim of this study was evaluate the in vitro activity heteroresistance characteristics Omad clinical S. isolates from China investigate resistance mechanisms. A sample 263 non-duplicate [127 methicillin-resistant (MRSA) 136 methicillin-sensitive (MSSA)] were collected...
This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis.Four four linezolid-sensitive E. faecalis which formed biofilms were collected for this study. Biofilm biomasses detected by crystal violet staining cells in mature quantified c.f.u. determination.Daptomycin alone, (4×MIC) demonstrated bactericidal activities on cells, killed more (at least 1-log10 ml-1) than alone. Daptomycin...
The aim of this study was to compare the effects radezolid and linezolid on planktonic biofilm cells Enterococcus faecalis. A total 302 E. faecalis clinical isolates were collected, minimum inhibitory concentrations (MICs) determined by agar dilution method. Changes in transcriptome a high-level, vitro-induced linezolid-resistant isolate assessed RNA sequencing RT-qPCR, roles efflux pump-related genes confirmed overexpression analysis. Biofilm biomass evaluated crystal violet staining...
Introduction. Staphylococcus aureus biofilms are difficult to treat and the effect of telithromycin treatment is still unclear. Aim. This study aimed explore against compared with azithromycin, clindamycin, vancomycin daptomycin. Methodology. Eight methicillin-susceptible eight methicillin-resistant S. isolates (MSSA MRSA, respectively) were used for this study. Biofilm biomasses detected by crystal violet staining adherent cells in established quantified determination colony-forming units...
This study aimed to evaluate the in vitro antimicrobial activity, heteroresistance emergence, and resistance mechanism of omadacycline (OMC) clinical Enterococcus faecalis isolates from China. A total 276 were collected retrospectively China 2011 2015. The MICs OMC, doxycycline (DOX), minocycline (MIN) against E. determined by broth microdilution. Tetracycline (TET)-specific genes multilocus sequence typing (MLST) investigated using PCR. detection frequency OMC was evaluated with population...
This study aims to evaluate the antimicrobial activities of linezolid and radezolid against Streptococcus agalactiae in vitro compared for genetic resistance factors.Nonduplicate S. clinical isolates (n = 136) were collected minimal inhibitory concentrations antimicrobials determined by agar dilution methodology. The linezolid-resistant mechanism linezolid-non-susceptible that induced pressure analyzed PCR sequence alignment. Antimicrobial distinctions between further investigated vitro.Our...
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Telithromycin has been reported to possess robust in vitro antibacterial activity against many species of gram-positive bacteria, and telithromycin is also effective Staphylococcus aureus biofilms. However, the antimicrobial susceptibility clinical enterococci isolates China rarely impacts on biofilm formation eradication remain elusive. Therefore, this study aimed explore inhibitory effects planktonic cells biofilms Enterococcus strains. A total 280 faecalis 122 faecium were collected from...
Tigecycline, eravacycline, and omadacycline are recently developed tetracyclines. Susceptibility of microbes to these tetracyclines their molecular mechanisms have not been well elucidated. We investigated the susceptibility Moraxella catarrhalis tigecycline, its resistance against tetracyclines.A total 207 non-duplicate M. isolates were collected from different inpatients. The minimum inhibitory concentrations (MICs) determined by broth microdilution. Tigecycline-, eravacycline-, or...
The intracellular aggregation of α-synuclein in neurons/glia is considered to be a key step the pathogenesis synucleinopathy [including Parkinson's disease (PD), dementia with Lewy body (DLB), multiple system atrophy (MSA), etc.]. Increasing evidence indicates that initial pathological aggregates can replicate themselves and propagate "seeding" manner areas brain even peripheral tissue, which makes it most important biomarker for diagnosis synucleinopathies recent years. amplification...
Corona virus disease 2019 (COVID-19) is a severe acute respiratory caused by syndrome coronavirus 2 (SARS-CoV-2). Different pooling testing strategies have been applied for the detection of SARS-CoV-2. However, discrepancies among different are still to be explored.The aim this study was evaluate two collecting specimens SARS-CoV-2 RNA. Two groups five-sample pools were prepared impact sample and pooled sampling on test sensitivity, respectively. Viral RNA extracted with automation system....
Abstract Background ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of Enterococcus faecalis remains obscure. A clpP deletion mutant (△ ) was constructed using E. OG1RF strain to clarify effect on faecalis. The global abundance proteins determined a mass spectrometer with tandem tag labeling. Results Δ showed impaired at 20°C 45°C 5% NaCl 2 mM H O...
Abstract Background : Enterococci has resistant to a wide range of antimicrobials, and the treatment enterococcal infection always been an issue concern. This study aimed explore new ketolides antimicrobials-telithromycin, against planktonic cells biofilms enterococci. The minimum inhibitory concentration (MIC) telithromycin was determined. sequence types (STs) genotypes resistance erythromycin in enterococci were detected. Furthermore, effect investigated. Results: A total 280 Enterococcus...