A5033882905- Reproductive Biology and Fertility
- Ovarian function and disorders
- Birth, Development, and Health
- Animal Genetics and Reproduction
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Metabolism and Genetic Disorders
- Renal and related cancers
- Magnetic and Electromagnetic Effects
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Prenatal Screening and Diagnostics
- Reproductive System and Pregnancy
- Renal cell carcinoma treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Sarcoma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Reproductive Health and Technologies
- Cancer Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Microbial Inactivation Methods
- Cancer Genomics and Diagnostics
- Collagen: Extraction and Characterization
Futaba clinic
2009-2024
Nagoya University Hospital
2024
Osaka Nishi Clinic
2006-2023
Nagoya University
2019-2022
Komaki City Hospital
2018-2022
Kashiwa Municipal Hospital
2020
Gifu Prefectural Tajimi Hospital
2015-2019
Kumamoto University
2011-2012
Yamagata University
2011
St. Marianna University School of Medicine
2009
One of the most critical issues to be solved in reproductive medicine is treatment patients with multiple failures assisted caused by low-quality embryos. This study investigated whether mitochondrial transfer human oocytes improves embryo quality and provides subsequent acceptable clinical results normality children born due use this technology. We transferred autologous mitochondria extracted from oogonia stem cells mature sperm at time intracytoplasmic injection 52 recurrent (average 5.3...
Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive humans. We investigated whether using a combination of DGC could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males fractionated by following DGC. Ultrastructural abnormalities original semen, lower layer DGC, upper analyzed transmission electron microscopy. The correlation...
Although it is not a well-established technology, oocyte cryopreservation becoming prevalent in assisted reproductive technologies response to the growing demands of patients' sociological and pathological conditions. Oocyte can adversely affect developmental potential oocytes by causing an increase intracellular oxidative stresses damage mitochondrial structure. In this study, we studied whether autologous adipose stem cell (ASC) mitochondria supplementation with vitrified warmed could...
Age-dependent decline of mitochondrial function has been proposed to be a main cause embryo quality. Then, l-carnitine plays important roles in reducing the membranous toxicity free-fatty acids by forming acyl-carnitine and promoting β-oxidation, preventing cell damage. Recent research revealed that played vitro oocyte growth, maturation development. However, such beneficial effects vivo have yet verified. The effect oral supplementation on quality implantation potential was examined. A...
Although in vitro maturation (IVM) of oocytes is important for assisted reproduction, the rate development embryos from IVM lower than their vivo counterparts. It has been shown that an artificial increase intracellular cAMP before culture significantly improves oocyte developmental competence cattle and mice. Here, we revealed forskolin 3-isobutyl-1-methylxanthine treatment prophase-stage induced expression genes required glycolysis, fatty acid degradation, mitochondrial electron transport...
Background: Oligometastatic cancer (OM) is possibly associated with relatively better survival outcomes. We attempted to identify cases in line this OM concept. Patients and Methods: A total of 130 unresectable metastatic pancreatic underwent non-curative surgery from April 2001 December 2019. Sites metastasis, clinicopathological information, surgical outcomes were collected formulate a definition OM. Results: criteria defined as having metastasis single organ, few countable lesions low...
Meiotic maturation of oocytes requires a variety ATP-dependent reactions, such as germinal vesicle breakdown, spindle formation, and rearrangement plasma membrane structure, which is required for fertilization. Mitochondria are accordingly expected be localized to subcellular sites energy utilization. Although microtubule-dependent cellular traffic mitochondria has been studied extensively in cultured neuronal (and some other somatic) cells, the molecular mechanism their dynamics mammalian...
The acid deoxyribonucleases [DNase II; EC 3.1.4.6] in human urine were purified approximately 400- to 500-fold by phosphocellulose chromatography, gel filtration on Sephadex G-75 and isoelectric focusing, with a total recovery of 22%. enzymes present least three forms different points, pHs 6.4, 6.6, 6.8. However, other properties essentially similar. did not require divalent cations for activity, the optimal at 5.1 5.3 33 mM acetate buffer. They had molecular weight around 36,000, as...
The effects of nanosecond pulsed electric field (ns PEF) on the egg development medaka fish are investigated. A power modulator using a magnetic pulse compression circuit was employed to generate pulses 0.5-20 kV pulses. Fertilized eggs strain d-rR were used. ages experimental new laid, 24-h postfertilization, and 48-h postfertilization. Propidium iodide fluorescein isothiocyanate (FITC)-dextran conjugate, fluorescence microscope used study ns PEF blastomeres extent structure damage. FITC...
The effects of the presence bovine leukemia inhibitory factor (bLIF) in culture medium on hatching rate and cell number inner mass (ICM) trophectoderm (TE) embryos were examined. Bovine presumptive zygotes divided into four groups which or absence 10ng/ml bLIF, period bLIF was throughout from day 0 to 5 and/or 10 (the vitro fertilization decided as 0). cleavage at 2 not significantly different among groups. Hatching rates cultured higher than those 10. numbers TE hatched blastocysts obtained...
The effect of bovine leukemia inhibitory factor (bLIF) on hatching and numbers inner cell mass (ICM) trophectoderm (TE) intact biopsied blastocysts was examined. Experiment 1: produced in vitro were cultured with 0, 2, 10, 25, 50 100ng/ml bLIF. rate varied concentrations bLIF significantly higher than that the without TE cells surviving medium increased compared However, there no differences ICM cells. 2: remaining embryos for 72h 10ng/ml There survival rates cultures or number culture These...
症例は63歳の女性で,検診で腹部超音波検査を受けた際に,膵腫瘍を指摘された.CTで膵尾部腹側に33 mmの低吸収腫瘤を認めた.病変は漸増性にやや不均一に造影された.MRIでは,T1強調像で低信号,T2強調像では中心部が低信号,辺縁部が淡い高信号,拡散強調像では辺縁部で高信号,中心部で低信号を呈した.以上の所見から,solid pseudopapillary neoplasm(SPN)やデスモイド腫瘍が疑われたが確定診断には至らず,診断的治療目的に手術の方針とした.術中所見では,腫瘍は膵尾部に存在し,胃大彎側の胃壁に浸潤していた.悪性腫瘍も疑われたため,腹腔鏡下脾合併膵体尾部切除,胃部分切除を施行した.術後病理組織診でデスモイド腫瘍と診断された.腹腔鏡下膵切除を施行したデスモイド腫瘍の報告はまれであり報告する.
Endometriomas (chocolate cysts) are cystic lesions that can develop on ovaries, and characterized by the presence of ectopic endometrial tissue or similar tissue. Such cause a decline in number quality oocytes, lead to implantation failure. In this study, we retrospectively assessed efficacy repeated endometrioma aspiration dienogest combination therapy patients suffering endometriosis-associated infertility with endometriomas. A comparison was made between treated group underwent followed...
To determine the optimum culture duration for porcine growing oocytes (GOs) to attain maturation competence, we examined meiotic chromatin configuration, and fertilization ability of obtained from early antral follicles cultured 10–16 days. The survival rate after 10 days (62.8%) was similar that 12 (55%) significantly higher than 14 16 (52.9 24.3%, respectively). No significant difference observed in diameter ooplasm different durations (117.4–118.3 μm). rates surviving (38.3 22.7%,...