A5027457499- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Retinal and Macular Surgery
- Angiogenesis and VEGF in Cancer
- Retinal Development and Disorders
- Ocular Diseases and Behçet’s Syndrome
- Intraocular Surgery and Lenses
- Microbial Inactivation Methods
- Lipid metabolism and disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Virus-based gene therapy research
- Reproductive biology and impacts on aquatic species
- Atherosclerosis and Cardiovascular Diseases
- Connexins and lens biology
- Immune cells in cancer
- Vascular Malformations Diagnosis and Treatment
- Natural Language Processing Techniques
- Toxin Mechanisms and Immunotoxins
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Cerebral Venous Sinus Thrombosis
Fukuoka Dental College
2022-2025
Insight (China)
2024
Kyushu University
2013-2022
Saiseikai Fukuoka General Hospital
2020
Saga University
2020
Fukuoka University
2018-2019
Osaka University
2013-2018
Nara Institute of Science and Technology
2016
Waseda University
2009
Hokkaido University
2007
Understanding molecular mechanisms regulating angiogenesis may lead to novel therapies for ischemic disorders. Hypoxia-inducible factor 1 (HIF-1) activates vascular endothelial growth (VEGF) gene expression in hypoxic/ischemic tissue. In this study we demonstrate that exposure of primary cultures cardiac and cells hypoxia or AdCA5, an adenovirus encoding a constitutively active form HIF-1alpha, modulates the genes angiogenic factors angiopoietin-1 (ANGPT1), ANGPT2, placental factor,...
Inflammation affects the formation and progression of various vitreoretinal diseases. We performed a comprehensive analysis inflammatory immune mediators in vitreous fluids from total 345 patients with diabetic macular edema (DME, n = 92), proliferative retinopathy (PDR, 147), branch retinal vein occlusion (BRVO, 30), central (CRVO, 13) rhegmatogenous detachment (RRD, 63). As control, we selected 83 either idiopathic hole (MH) or epiretinal membrane (ERM) that were free major pathogenic...
<h3>AIM</h3> To determine the prevalence of age related maculopathy (ARM) in a representative older Japanese population. <h3>METHODS</h3> 1486 residents Hisayama town, Fukuoka, Japan, aged 50 years or were examined and presence ARM was determined by grading from fundus examination indirect ophthalmoscope, slit lamp, colour photographs. <h3>RESULTS</h3> The rate drusen, which occurred with comparable frequency men women, 9.6%. drusen increased (p <0.01). Hyperpigmentation and/or...
To compare the 1-year results of initial or deferred photodynamic therapy (PDT) combined with intravitreal ranibizumab (IVR) for eyes polypoidal choroidal vasculopathy.Prospectively, 72 men treatment-naive vasculopathy were randomized to later PDT IVR. In both groups, 2 additional monthly IVR followed. From Month 3, and administered according retreatment criteria. Mean changes in best-corrected visual acuity between baseline 12 central retinal thickness, rate showing regression lesions,...
Although apoptosis is assumed to play a pivotal role in retinal function loss, its mechanism and real influence on are still unclear. To investigate the relation between apoptosis, we studied photoreceptor experimental detachment (RD).We induced RD by subretinal injection of sodium hyaluronate Brown Norway rats. Apoptotic photoreceptors were detected TdT-dUTP Terminal Nick-End Labeling (TUNEL). evaluate detached retina, electroretinograms (ERGs) taken day 1, 3 with corneal electrodes...
Endostatin, a proteolytic fragment of collagen XVIII, is an endogenous inhibitor tumor angiogenesis that also inhibits choroidal neovascularization. In this study, we assessed the effects increased intraocular expression endostatin on vascular endothelial growth factor (VEGF)-induced changes in retina. After subretinal injection pair gutless adenoviral vectors (AGV) designed to provide tamoxifen-inducible endostatin, diffuse immunoreactivity was induced thoroughout retina by administration...
Choroidal neovascularization (CNV) is a characteristic of age-related macular degeneration. Genome-wide association studies have provided evidence that the immune system involved in pathogenesis degeneration; however, role inflammatory cytokines CNV has not been established. In this study, we demonstrated IL-17 had strong potential for promoting vascular endothelial growth factor-independent manner laser-induced experimental mice. Infiltrated γδT cells and Thy-1(+) innate lymphoid cells, but...
Choroidal neovascularization (CNV) is a serious complication of age‑related macular degeneration. The aim the present study was to investigate expression and distribution M1 M2 macrophages in laser‑induced CNV adult mouse model. mRNA levels M1, pan macrophage markers, macrophage‑associated angiogenic cytokines, were determined by reverse transcription‑quantitative polymerase chain reaction. Immunofluorescence studies performed determine location macrophages. markers increased greater extent...
Purpose: To investigate the roles played by M2 macrophages in a mouse model of oxygen-induced retinopathy (OIR). Methods: Oxygen-induced was induced C57BL/6J mice exposing postnatal day seven (P7) pups to 75% oxygen and then returning them room air at P12. Real-time RT-PCR immunofluorescence staining were used assess levels distributions different macrophage markers. Bone marrow–derived M1 mannosylated clodronate liposomes (MCLs) injected into vitreous on P12 examine effects P17. cocultured...
Purpose.: We determined the profile of genes expressed in fibrovascular membranes (FVMs). Methods.: Six FVMs were surgically removed from patients with proliferative diabetic retinopathy (PDR) during pars plana vitrectomy membrane peeling. The classified into three active or inactive according to presence absence neovascularization (NV) membranes. Total RNA was isolated six and also normal human retinas. DNA microarray analysis performed compare those retinas, between FVMs. Ingenuity pathway...
purpose. Topical nepafenac readily penetrates the cornea and is metabolized to amfenac, a potent cyclooxygenase (COX)-1 COX-2 inhibitor. In this study, we tested effect of topical in three murine models ocular neovascularization (NV). methods. A masked trial was performed compare effects vehicle with one several concentrations (0.01%, 0.03%, 0.1%, or 0.5%), 0.1% diclofenac, 0.5% ketorolac tromethamine mice oxygen-induced ischemic retinopathy, choroidal NV (CNV) due laser-induced rupture...
Abstract We studied the chronic effects of 4-nonylphenol (4-NP) on reproductive status medaka (Oryzias latipes) over two generations continuous exposure. The exposure study parental (F0) was begun embryos within 24 h postfertilization and continued with monitoring through embryological development, hatching, posthatch survival, growth, sexual differentiation, reproduction under flow-through exposures to mean measured 4-NP concentrations 4.2, 8.2, 17.7, 51.5, 183 μg/L for up 104 d. Eggs...
In this study, we used double transgenic mice with inducible expression of angiopoietin-2 (Ang2) to investigate the role Ang2 in retinal and choroidal circulations three models ocular neovascularization (NV). Mice induced ubiquitously, or specifically retina, survived appeared grossly normal. They also had normal-appearing circulations, demonstrating that high levels did not induce regression mature vessels. When was soon after birth, there increased density deep capillary bed on postnatal...
Abstract Increased expression of vascular endothelial growth factor (VEGF) in the retina starting after postnatal day (P)7 results neovascularization originating from deep retinal capillaries, but not those superficial capillary bed. Doxycycline was administered P0 to double transgenic mice with inducible VEGF retina. These showed proliferation and dilation indicating that at this stage development, capillaries are sensitive effects VEGF. Angiopoietin‐2 (Ang2) is expressed along surface for...
Abstract Retinal neovascularization (NV) and macular edema, resulting from blood‐retinal barrier (BRB) breakdown, are major causes of visual loss in ischemic retinopathies. Choroidal NV (CNV) occurs diseases the retinal pigmented epithelium/Bruch's membrane complex is another extremely prevalent cause loss. We used mice which hypoxia response element (HRE) deleted vascular endothelial growth factor ( vegf ) promoter (Vegf δ/δ mice) to explore role induction VEGF through HRE these disease...
Proliferative vitreoretinopathy (PVR) is a severe, vision-threatening disorder characterized by the fibrous membrane formation that leads to tractional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of membranes revealed significant up-regulation periostin. We also found increased periostin expression in vitreous and pigment epithelial (RPE) cells from PVR patients. vitro, proliferation, adhesion,...
To determine whether vitreal concentrations of MCP-1, IL-6 and IL-8 are altered after vitrectomy in patients with proliferative diabetic retinopathy (PDR) to investigate the levels these cytokines associated postoperative macular oedema.Vitreous samples were collected from 36 eyes 33 PDR before pars plana without intraocular lens (IOL) implantation, also same during IOL implantation surgery approximately 7 months initial vitrectomy. Levels IL-6, vascular endothelial growth factor measured by...
Age-related macular degeneration (AMD) is a major cause of blindness in the elderly. Previous sequencing studies AMD susceptibility genes have revealed association rare coding variants CFH, CFI, C3 and C9 European population; however, impact or low-frequency on other populations largely unknown. To identify role exudative Japanese population, we analysed 34 candidate two-stage design by multiplex PCR-based target method. We used total 2,886 (1st: 827, 2nd: 2,059) cases including typical AMD,...
Purpose: This study aimed to evaluate the outcomes of aflibercept treatment using a treat-and-extend (TAE) regimen over up 5 years in Japanese patients with neovascular age-related macular degeneration (nAMD). Patients and Methods: retrospective included 126 consecutive treatment-naïve nAMD (126 eyes) who received at least three monthly intravitreal (IVA) injections as loading phase, followed by follow-up for years. Injection intervals were adjusted every 2 weeks could be extended 16 based...
Abstract Increased expression of vascular endothelial cell growth factor (VEGF) in the retina is sufficient to stimulate sprouting neovascularization from deep capillary bed retina, but not superficial retinal capillaries or choriocapillaris. Coexpression VEGF and angiopoietin 2 (Ang2) results capillaries, However, retina‐derived Ang2 may reach choriocapillaris, because tight junctions between pigmented epithelial (RPE) cells. To eliminate this possible confounding factor, we used human...