A5058924690- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Syphilis Diagnosis and Treatment
- Insect symbiosis and bacterial influences
- Reproductive tract infections research
- Synthesis and Biological Evaluation
- Virology and Viral Diseases
- Science and Education Research
- Parasites and Host Interactions
- Parasitic Diseases Research and Treatment
- Medical Coding and Health Information
- Chemistry Education and Research
- Studies on Chitinases and Chitosanases
- Viral Infections and Immunology Research
- Sex work and related issues
- Mosquito-borne diseases and control
Fundação Oswaldo Cruz
2019-2025
Fundação Carlos Chagas
2024
Background In Brazil, acute Chagas disease (ACD) surveillance involves mandatory notification, which allows for population-based epidemiological studies. We conducted a nationwide ecological analysis of the spatiotemporal patterns ACD notifications in Brazil using secondary data obtained from Notifiable Diseases Information System (SINAN) maintained by Brazilian Ministry Health. Methodology/Principal findings this all cases reported between 2001 and 2018 were included. Epidemiological...
Chimeric T. cruzi antigens have been proposed as a diagnostic tool for chronic Chagas disease (CD) in both settings where is endemic and those it not endemic. Antibody response varies accordance to each strain, presenting challenges the use of lacking demonstrated cross-reactivity with Leishmania spp.
In Brazil, the notification of congenital (CS) and syphilis in pregnant women (SiP) is compulsory. Notification data provided by Ministry Health combination with mapping vulnerable geographic areas essential to forecasting possible outbreaks more effectively combating infection through monitoring. We aim evaluate spatiotemporal distribution epidemiological aspects reported cases CS SiP Brazil. A retrospective ecological study was carried out using secondary surveillance obtained from...
Syphilis, a sexually transmitted infection caused by the bacterium Treponema pallidum, has high incidence rates among adults, pregnant women, and newborns. Diagnostic procedures typically involve treponemal test (such as ELISA, CMIA, IFI), followed non-treponemal (VDRL RPR). This study aimed to assess diagnostic performance of double antigen sandwich ELISA (DAgS-ELISA) using recombinant protein TpN17, analyzing serum samples from both infected not with T. pallidum. A total 712 were deemed...
Syphilis is a sexually transmitted infection (STI) caused by the spiral bacterium Treponema pallidum . Diagnosis based on epidemiology, clinical and serology, but serodiagnosis challenging because distinct forms of may influence serological performance. Several recombinant -proteins have already been tested for syphilis diagnosis they are critical to achieve high accuracy in testing. A total 647 samples were included study: 180 T. -positive samples, 191 -negative 276 sera from individuals...
Despite several available methodologies for Chagas disease (CD) serological screening, the main limitation of chronic CD diagnosis is lack effective tools large-scale screening and point-of-care to be used in different epidemiological scenarios. Taking into account that developing such a diagnostic tool will significantly improve ability identify carriers, we aimed at performing proof-of-concept study (phase I study) assess use these proteins platform using serum samples from geographical...
Background Enzyme-linked immunosorbent assays (ELISA) are generally the chosen test for Chagas disease (CD) diagnosis; however, its performance depends on antigen preparation adsorbed to solid phase, which may lead false-positive results and cross-reactions. The use of chimeric recombinant antigens can overcome this limitation. Four from Trypanosoma cruzi (IBMP-8.1, IBMP-8.2, IBMP-8.3 IBMP-8.4) were developed evaluated in phase I, II III studies using indirect ELISA as diagnostic platform....
Background Dogs are considered sentinels in areas of Trypanosoma cruzi transmission risk to humans. ELISA is generally the method choice for diagnosing T. exposure dogs, but its performance substantially depends on antigenic matrix employed. In previous studies, our group has developed four chimeric antigens (IBMP-8.1, 8.2, 8.3, and 8.4) evaluated their potential For human sera, these presented superior diagnostic performances as compared commercial tests available Brazil, Spain, Argentina....
Chagas disease (CD) is among the top 10 causes of inability to blood donation. Blood donation centers screen for anti-Trypanosoma cruzi antibodies using highly sensitive immunoenzymatic (ELISA) or chemiluminescent methods, which can lead false positive results. Since samples cannot be used, avoid loss valuable donations, it necessary improve specificity without reducing sensitivity tests used screening. For this purpose, our group has developed four chimeric proteins (IBMP-8.1, IBMP-8.2,...
Background Chagas disease (CD) is caused by Trypanosoma cruzi . The chronic phase of CD characterized the presence IgG anti- T antibodies; and diagnosis performed serological methods. Because there no reliable test that can be used as a reference test, WHO recommends parallel use two different tests for serodiagnosis. If results are inconclusive, samples should subjected to confirmatory e.g., Western blot (WB) or PCR. PCR offers low sensitivity in phase, whereas few based on WB method...
Abstract Background Chagas disease (CD) serological screening at blood banks is usually performed by a single highly sensitive assay, with chemiluminescent immunoassays (CLIAs) being the method of choice. CLIAs employ recombinant, fusion peptides and/or chimeric antigens that selectively capture anti‐ Trypanosoma cruzi antibodies. However, despite high sensitivity, ability these tests to identify CD‐positive cases should be evaluated against T. strains circulating in specific locales....
Chagas disease (CD) is a neglected caused by Trypanosoma cruzi Chagas, 1909. Causative treatment can be achieved with two drugs: benznidazole or Nifurtimox. There are some gaps that hinder progress in eradicating the disease. no test efficiently assess cure control after treatment. Currently, decline anti-T. antibody titres assessed conventional serological tests, which take years. However, search for new markers of must continue to fill this gap. The present study aimed evaluate using...
There are a variety of nontreponemal test (NTT) and treponemal (TT) kits for the serologic diagnosis syphilis. Because complexity infection (multiple clinical stages) different antigens used in these kits, systematic evaluation accuracy currently available commercial tests is warranted. Our objective was to evaluate performance commercially syphilis infection. In this study, we analyzed one NTT (Venereal Disease Research Laboratory [VDRL] test, Wiener Laboratories, Rosario, Argentina) two...
Abstract Background Chagas disease (CD), a neglected parasitic caused by Trypanosoma cruzi , poses significant health threat in Latin America and has emerged globally because of human migration. infects humans over 100 other mammalian species, including dogs, which are important sentinels for assessing the risk infection. Nonetheless, serodiagnosis T. dogs is still impaired absence commercial tests. In this study, we investigated diagnostic accuracy four chimeric recombinant IBMP antigens...
Chagas disease (CD), a neglected tropical caused by Trypanosoma cruzi , is significant public health issue particularly in Latin America, affecting millions worldwide. Diagnosis challenge owing to the genetic diversity of T. and complexities involved selecting antigens for detection anti– antibodies. This study evaluated four chimeric recombinant (IBMP-8.1, IBMP-8.2, IBMP-8.3, IBMP-8.4) designed enhance diagnostic accuracy addressing assay variability. We compared performance these using...
The performance of an immunoassay relies on antigen-antibody interaction; hence, antigen chemical stability and structural integrity are paramount for efficient assay. We conducted a functional, thermostability long-term analysis different chimeric antigens (IBMP), in order to assess effects adverse conditions four employed ELISA diagnose Chagas disease. ELISA-based immunoassays have served as model biosensors development, both molecular interactions. To evaluate thermostability, samples...
Abstract Background. In Brazil, the notification of acquired syphilis (AS), congenital (CS) and in pregnant women (SiP) is compulsory. Notification data provided by Ministry Health (MoH) combination with mapping vulnerable geographic areas essential to forecasting possible outbreaks more effectively combating infection through monitoring. We aim evaluated space time distribution reported cases CS SiP Brazil (2001 2017), as well an estimation AS (2010 2018). Methods. A retrospective...
Abstract Background. In Brazil, the notification of acquired syphilis (AS), congenital (CS) and in pregnant women (SiP) is compulsory. Notification data provided by Ministry Health (MoH) combination with mapping vulnerable geographic areas essential to forecasting possible outbreaks more effectively combating infection through monitoring. We aim evaluated space time distribution reported cases CS SiP Brazil (2001 2017), as well an estimation AS (2010 2018). Methods. A retrospective...