A5066537259- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Tissue Engineering and Regenerative Medicine
- Nuclear Receptors and Signaling
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- GDF15 and Related Biomarkers
- Testicular diseases and treatments
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
- Ubiquitin and proteasome pathways
- Additive Manufacturing and 3D Printing Technologies
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Prostate Cancer Treatment and Research
- Immune Response and Inflammation
- Microfluidic and Capillary Electrophoresis Applications
- Hippo pathway signaling and YAP/TAZ
- Nanopore and Nanochannel Transport Studies
- Urologic and reproductive health conditions
- Cancer Genomics and Diagnostics
Charles River Laboratories (United States)
2023-2024
Michigan Medicine
2022
University of Michigan–Ann Arbor
2013-2020
Zero to Three
2014
Johns Hopkins Medicine
2009-2013
Johns Hopkins University
2009-2013
University of Baltimore
2012
The metastatic invasion of cancer cells from primary tumors to distant ecological niches, rather than the tumors, is cause much mortality [Zhang QB, et al. (2010) Int J Cancer 126:2534–2541; Chambers AF, Goss PE (2008) Breast Res 10:114]. Metastasis a three-dimensional process where spread their site origin and colonize microenvironmental niches. It critical be able assess quantitatively potential [Harma V, PLoS ONE 5:e10431]. We have constructed microfabricated chip with topology consisting...
Abstract Cancer cells typically demonstrate altered morphology during the various stages of disease progression as well metastasis. While much is known about how cell in cancer a result genetic regulation, less changes affect function by influencing gene expression. In this study, we different types disrupting actin cytoskeleton or modulating attachment and observed rapid up‐regulation growth differentiation factor 15 (GDF15), member transforming factor‐beta (TGF‐β) super‐family. Strikingly,...
The analysis of invading leader cells at the tumor invasion front is significant interest as these may possess a coordinated functional and molecular phenotype which can be targeted for therapy. However, such analyses are currently limited by available technologies. Here, we report fluidic device long-term three-dimensional tumoroid culture recapitulated front, allowing both quantification invasive potential characterization cells. Preliminary indicated an association with cell proliferation...
Abstract Identifying better predictive and prognostic biomarkers for the diagnosis treatment of triple negative breast cancer (TNBC) is complicated by tumor heterogeneity ranging from responses to therapy, mutational burden, clonal evolution. To overcome gap in our understanding heterogeneity, we hypothesized that isolating studying gene expression profile invasive cell subpopulations would be a crucial step towards achieving this goal. In report, utilized fluidic device previously reported...
Abstract Currently, there are no specific markers available for the early detection and monitoring testicular cancer. Based upon an approach that targets nuclear structure, we have identified a set of proteins seminomas, which may then clinical utility disease. Utilizing samples obtained from men with evidence cancer (n = 5) as well those seminomas 6), matrix were extracted separated using high‐resolution two‐dimensional electrophoresis gel system. The by mass spectrometry analysis. These...
The bone is a mechanosensitive organ that also common metastatic site for prostate cancer. However, the mechanism by which tumor interacts with microenvironment to further promote disease progression remains be fully understood. This largely due lack of physiological yet user-friendly models limit our ability perform in-depth mechanistic studies. Here, we report tunable bioreactor facilitates 3D culture osteocyte cell line, MLO-Y4, in hydroxyapatite/tricalcium phosphate (HA/TCP) scaffold...
Abstract Cell attachment to the extracellular environment is critical in determining cell fate. When normal cells lose attachment, they undergo anoikis. Cancer develop resistance anoikis which results metastasis. While there are many known genes alterations that can alter adhesion, less about how such a loss of adhesion may influence gene expression. We believe change could trigger changes expression, and resulting play an important role prostate cancer Using lines, we examined expression...
Abstract Introduction: Prostate cancer is the most prevalent cause of deaths in American males after lung cancer. The establishment clinically relevant prostate models crucial for advancing our understanding disease and evaluating potential therapeutics. Oncology studies rats provide a secondary species to mice with advantage extensive longitudinal blood sampling tumor biopsies pharmacodynamics analysis. This vivo study evaluates behavior subcutaneous orthotopic luciferase (luc) tagged...
Abstract Immunodeficient rodents are vital pre-clinical disease models. Historically, immunodeficient mice have been standard for hosting human cell and tissue xenografts, enabling cancer drug efficacy tolerability testing. However, an rat that supports a variety of types provides larger model easier surgical manipulation serial blood tumor sampling, allowing efficacy, pharmacokinetics, toxicology testing in the same animal. We created Sprague Dawley Rag2 -/-, Il2rg -/- (SRG Rat®; SRG) is...
Abstract Introduction The number of approved cancer drugs as well those in development have doubled over the last decade. With this increase comes a need for validated immunodeficient animal models that allow engraftment tumor xenografts, an important role drug process. Immunodeficient rats are suited studies require serial tissue sampling such blood. Under NIH guidelines, up to 88µl whole blood from 125g rat can be collected per day compared 21µl 30g mouse. Tumor size endpoints also larger...
<h3>Background</h3> The number of cancer drugs in development has doubled over the last decade, increasing need for well characterized immunodeficient animal models that allow human xenograft engraftment, an important component drug process. With their larger organism size, rats are suited studies require serial sampling tissues such as blood and tumor, or surgical procedures complicated by small size mice. SRG is rat with deletions recombination activating gene 2 (<i>Rag2</i>) interleukin...
In this talk, I will discuss our recent effort using synthetic micro/nanoengineered tools to study mechano-sensitive and -responsive behaviors of human pluripotent stem cells (hPSCs) the underlying molecular cellular mechanisms.
Abstract To realize the promise of precision medicine, it is important to integrate phenotypic assessment cell populations genomic data. The analysis invading leader cells at tumor invasion front interest as they may be guided by a targetable molecular phenotype. However, there lack suitable platforms on which analyze front. In this study, we have designed and constructed fluidic device for long-term (several days weeks) 3-dimensional tumoroid culture diverse cancer cells. Using device, can...