Objective: The Newcastle disease virus (NDV) is an infectious that causes very high eco¬nomic losses due to decreased livestock production and poultry deaths. vaccine’s ineffec¬tiveness mutation of the genetic structure impacts obstacles in controlling disease, especially some endemic areas. This study aimed provide alternative treatment for NDV infection by observing viral replication inhibitor activity Clostridium perfringens sialidase primary chicken embryo fibroblast (CEF) cells....

The avian influenza virus is an infectious agent that may cause global health problems in poultry and potentially zoonotic. In the recent decades, bacterial-derived sialidases have been extensively studied for their ability to inhibit infections. this study, antiviral activity of NanB sialidase from

Introduction: Some people living with HIV (PLWH) receiving ART in Indonesia display poor clearance of replicating virus. This has been associated HIV-associated sensory neuropathy. Here we assess whether treatment failure reflects the presence drug resistance mutations. Methodology: PLWH were stratified by RNA levels using a ≥ 1000 copies/mL cut-off after 5.3 (2-7.5) years on ART. Drug-resistance mutations analyzed seven ten cases detectable viral load. The pol gene was screened for...

Background: Reprogrammed cell therapy has not been applied for clinical purposes due to the malignancy issue. The aim of this study was design recombinant vector transcription factors and analyze effectiveness cell-penetrating peptide delivering system human primary fibroblast transfection avoid issue.Materials methods: constructions CCAT/enhancer binding protein alpha (CEBPA), hepatocyte nuclear factor 4 (HNF4A), receptor subfamily 1 group I member 2 (NR1I2) were confirmed with DNA...

Abstract The authors have requested that this preprint be removed from Research Square.

Cell penetrating peptides (CPP) are one of the most attractive DNA delivery systems currently in development. In this research, silico CPP development was performed based on a literature study to look for that induce endosome escape, have ability bind DNA, and pass through cell membranes and/or nuclear with final goal creating new be used as system. We report herein successful isolation three candidate molecules, which all been successfully expressed purified by NiNTA. One determinants...

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Background: High mutation rates in HIV-1 could affect the accuracy of diagnostic tests. Therefore, recombinant antigen that has an immunodominant and conserved region from need to be developed detect infection Indonesia.Materials methods: The antigens comprise Gag (p24), Pol Env (gp41). Each was expressed Escherichia coli expression system purified using Ni-NTA chromatography. reactivity against HIV antibodies tested a group 50 HIV-positive plasma samples 45 HIV-negative immunoblot assay...

Background: The M protein is one of the structural SARS CoV-2. relatively conserved and stable than other protein. This made immunodominant epitopes has advantage to be learned with aim understanding its immunogenicity antigenicity. Several studies have shown that successfully expressed in several expression system, E. coli was them. In this study, gene CoV-2 cloned, sequenced, BL21 (DE3) purified denature condition. membrane recombinant can used for development a antibody diagnostic...

Latar Belakang: Stimulasi respon sel T CD8+ spesifik Gag, terkait dengan penurunan viremia, kontrol replikasi virus, dan perkembangan penyakit yang lambat. Respon efektif juga dipengaruhi oleh CD4+. Protein rekombinan Gag dapat diklona diekpresikan pada sistem prokariota, saat diimunisasi hewan coba atau manusia akan bersifat sebagai antigen eksogen. Antigen eksogen menjadi endogen menambahkan protein mempunyai kemampuan bertranslokasi kedalam membran sel, salah satunya Vp22.Metode:...

Human immunodeficiency virus (HIV) is a that causes acquired syndrome (AIDS). The HIV genome has cap structure at 5’ and polyadenylation 3’ on mRNA resulting in translation initiation through scanning 5'untranslated region (UTR). Vpr protein produced during viral replication the 5'cap to be inhibited so HIV-1 can directly recruit ribosome start codon via internal ribosomal entry site (IRES). IRES activity high G2/M phase highest expression monocyte cell line (THP-1) lymphocyte (HPB-ALL)....

AbstractHepatitis C virus (HCV) infection can cause chronic liver disease that develops into cirrhosis and cancer. It is estimated are more than 170 million of th world’s population suffering from HCV. Accurate diagnosis needed to provide appropriate early treatmen, including preventing further transmission the virus. The purpose this study was construct plasmid expression recombinant antigen for detection anti-HCV antibodies. coding gene designed so composed epitopes immunodominant,...

There are many studies ongoing about the remarkable potential of pluripotent stem cell as future regenerative therapy. Embryonic is only source that has been studied well for it. However, constraints arise regarding this matter like ethical problems and risk transplantation rejection. Recently, there research undergone in exploring new approach through reprogramming somatic to become induced (iPSC). OCT4 (Octamer-binding transcription factor 4) one proteins responsible self-renewal embryonic...

The capability of DNA vaccine to transfect cells determines the success vaccine, but there are some natural obstacles that reduce efficiency delivery into cells. One way overcome this problem is through use a systems such as needle free injectors (NFIT). In present study, we evaluated 2 kinds NFITs, Injex and Twin-Jector EZII, control hypodermic syringes. path injection NFITs deliver were observed by using India ink plasmid coding enhanced green fluorescent protein (pCDNA eGFP) respectively....

Latar belakang: Secara alamiah protein kapsid L1 Human Papillomavirus (HPV) tipe 16 dapat mengalami auto assembly untuk membentuk Viral like particle (VLP). Terkait dengan penelitian vaksin HPV, VLP digunakan berbagai keperluan seperti vaksin, pseudovirion atau SpyTag-Spycatcher. Penelitian ini ditujukan mendapatkan plasmid rekombinan yang produksi HPV 16.
 Metode: Gen penyandi diklona ke dalam vector pQE80L, suatu mengandung sistem ekspresi prokariota. DNA disisipkan pada situs...

AIDS is a severe immunodeficiency disease caused by HIV. Identification of new HIV infection in population required for the evaluation intervention strategy HIV-1 transmission. The avidity assay has been promoted detection. Avidity based on affinity strength epitopes antigen against its specific corresponding antibodies. binding - antibody formed initial phase relatively weak and easy to break with chaotropic reagents. In contrary, antigen-antibody formation long-term strong not easily...

AbstractFrom 36.9 million people infected by the Human Immunodeficiency Virus (HIV) at end of 2018 in whole world, 1-2 are HIV-2. Accurately diagnostic is required to ensure whether an individual has been HIV-1, HIV-2, or HIV-1 and HIV-2 co-infection. error using rapid tests (RDT) frequently occurs. Misdiagnostic infection may cause treatment failure which leads development Acquired Immune Deficiency Syndrome (AIDS). In this study, expression plasmid pQE80L-gp125-gp36 that can produce...

Abstract The Early (E) 7 protein of Human Papillomavirus 16 (HPV16) is an important transformation agent that plays a role in the progression HPV infection to invasive lesion, cervical cancer.  The HPV16 E7 has significant assignment activation CD8 destroy cancer cells. Nevertheless, domain can activate protooncogenes pRB. To increase safety vaccine, this study, sequence been rearranged disrupt continuity pRB interaction by considering epitopes and CD4. sequences from patients reference...

BACKGROUND Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like-3G (APOBEC3G) can abolish HIV infection by inducing lethal mutations in the genome. The protein virion infectivity factor (Vif) interact with APOBEC3G and cause its degradation. Development of a method that screen substances inhibiting APOBEC3G-Vif interaction is necessary for identification potentially used anti-HIV drug development. In order to increase expression recombinant will be assay, we developed an optimized...

The evaluation of the tumor protein p53 (TP53) codon 72 polymorphism (rs1042522) status in a population exposed to high background radiation was performed this study. Real-time polymerase chain reaction (Q-PCR) used genotype rs1042522 100 subjects from Takandeang, Salleto, and Ahu villages Mamuju district, West Sulawesi. An silico study then conducted identify potential effects proline substitution associated with on stability. analysis using three different computational tools, namely...

Cell-penetrating peptides (CPPs) are small that can transfer other materials into a cellular compartment. In this research, we studied the effect of fusion new CPPs to N-terminal enhanced Green Fluorescent Protein eGFP on ability latter fluoresce. Results showed recombinant protein CPPs-eGFP could be successfully expressed in Escherichia coli. contrast E. coli expressing wild-type eGFP, which fluoresce under ultraviolet (UV) or visible light, lost their PyMol, molecular visualization system,...

Abstract Study on sialidases as antiviral agents has been widely performed, but many types of sialidase had not tested for their activity. One such is the NanB Pasteurella multocida, which never isolated further study. In this study, activity was investigated in silico by docking multocida to Neu5Acα(2-6)Gal ligand. Additionally, some local isolates , gene were screened, and proteins testing regarding hydrolyzing Neu5Acα(2-6)Gal. studies showed that possesses an exceptional affinity towards...

The endogenous HIV-1 vaccine based on Gag protein is expected to stimulate the immune response of CD8+ T cells (cytotoxic). that has been produced by E.coli prokaryote system an exogenous antigen. fusion VP22 deliver antigen into cytoplasm cell, observed eGFP markers. Sequences (114 pb), GagHIV-1 (1506 and (733 pb) were inserted pQE80L, respectively. recombinant was expressed in purified Ni-NTA method. Antigen delivery fused with fluorescence confocal microscopy. plasmid constructs...