A5023244558- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Supramolecular Self-Assembly in Materials
- Peptidase Inhibition and Analysis
- Radical Photochemical Reactions
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Proteoglycans and glycosaminoglycans research
Scripps Research Institute
2021-2023
Scripps Institution of Oceanography
2019-2021
Scripps (United States)
2019-2021
Torrey Pines Institute For Molecular Studies
2019
DNA Encoded Libraries have proven immensely powerful tools for lead identification. The ability to screen billions of compounds at once has spurred increasing interest in DEL development and utilization. Although provides access libraries unprecedented size diversity, the idiosyncratic hydrophilic nature tag severely limits scope applicable chemistries. It is known that biomacromolecules can be reversibly, noncovalently adsorbed eluted from solid supports, this phenomenon been utilized...
Advances in the modulation of protein-protein interactions (PPIs) enable both characterization PPI networks that govern diseases and design therapeutics probes. The shallow protein surfaces dominate PPIs are challenging to target using standard methods, approaches for accessing extended backbone structures limited. Here, we incorporate a rigid, linear, diyne brace between side chains at
To facilitate the characterization of phase-transitioning molecules, site-specific non-perturbative infrared probes are leveraged for continuous observation self-assembly fibrils in a peptide hydrogel following stopped-flow initiation.
Herein, we present the adaptation of reversible adsorption to solid support (RASS) for a DEL setting, which allows reactions be performed in organic solvents at near anhydrous conditions opening previously inaccessible chemical reactivities DEL. The RASS approach enabled rapid development C(sp<sup>2</sup>)-C(sp<sup>3</sup>) decarboxylative cross-couplings with broad substrate scope, an electrochemical amination (the first synthetic transformation context), and...
Herein, we present the adaptation of reversible adsorption to solid support (RASS) for a DEL setting, which allows reactions be performed in organic solvents at near anhydrous conditions opening previously inaccessible chemical reactivities DEL. The RASS approach enabled rapid development C(sp 2 )-C(sp 3 ) decarboxylative cross-couplings with broad substrate scope, an electrochemical amination (the first synthetic transformation context), and improved reductive conditions. We believe that...
The characterization of self-assembling molecules presents significant experimental challenges, especially when associated with phase separation or precipitation. Transparent window infrared (IR) spectroscopy leverages site-specific probes that absorb in the “transparent window” region biomolecular IR spectrum. Carbon-deuterium (C-D) bonds are compelling transparent since they non-perturbative, can be readily introduced site selectively into peptides and proteins, their stretch frequencies...
Advances in the modulation of protein-protein interactions (PPIs) enable characterization PPI networks that govern disease mechanisms and guide design novel therapeutics probes. These PPIs are often characterized by complementary binding to shallow protein surfaces challenging target using standard methods for high-affinity small molecule ligand generation. Compared linear peptides, synthetically constrained epitopes provide an energetic advantage decreasing unbound-state entropy. Such...
The physical characterization of molecules that self-assemble presents a challenge, particularly when associated with phase separation or precipitation. Infrared (IR) spectroscopy has been shown to be non-perturbative method for monitoring folding dynamics, accentuated by the sensitivity bond stretch frequencies site-specific IR probes changes in local molecular environment.1 Site-specific incorporation absorb “transparent window” region biomolecular spectrum widely used gain insight into...
The characterization of self-assembling molecules presents significant experimental challenges, especially when associated with phase separation or precipitation. Transparent window infrared (IR) spectroscopy leverages site-specific probes that absorb in the “transparent window” region biomolecular IR spectrum. Carbon-deuterium (C-D) bonds are compelling transparent since they non-perturbative, can be readily introduced site selectively into peptides and proteins, their stretch frequencies...