A5047861370- Systemic Lupus Erythematosus Research
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Neonatal Respiratory Health Research
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Mitochondrial Function and Pathology
- Erythrocyte Function and Pathophysiology
- Renal Diseases and Glomerulopathies
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- RNA regulation and disease
- Congenital Diaphragmatic Hernia Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood disorders and treatments
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Autoimmune and Inflammatory Disorders Research
- Advanced Glycation End Products research
- Rheumatoid Arthritis Research and Therapies
Sapienza University of Rome
2015-2025
Policlinico Umberto I
2011-2017
Istituto di Sociologia Internazionale di Gorizia
2015
University of Trieste
2015
Istituto Superiore di Sanità
2014
Medicina
2013
Hodges University
1975
American Congress of Obstetricians and Gynecologists
1975
American Society for Reproductive Medicine
1975
University of Naples Federico II
1975
Abstract Objective To investigate the association of β 2 ‐glycoprotein I (β GPI) with lipid rafts in monocytic cells and to evaluate proinflammatory procoagulant effects anti‐β GPI binding its target antigen on monocyte plasma membrane. Methods Human monocytes were fractionated by sucrose density‐gradient centrifugation analyzed Western blotting. Immunoprecipitation experiments performed analyze possible interaction annexin A2 Toll‐like receptor 4 (TLR‐4). Monocytes then stimulated...
Autophagy may represent a functional processing event that creates substrate for autoreactivity. In particular, autophagy play role in the pathogenesis of RA, since is key cellular involved generation citrullinated peptides, with consequent breakage tolerance. Thus, be common feature several situations (including smoking, joint injury and infection) drive adaptive responses to self-proteins. The aim this study was analysis, vitro, peptides and, vivo, relationship between production anti-CCP...
Mitochondria-associated membranes (MAMs) are essential communication subdomains of the endoplasmic reticulum (ER) that interact with mitochondria. We previously demonstrated that, upon macroautophagy/autophagy induction, AMBRA1 is recruited to BECN1 complex and relocalizes MAMs, where it regulates autophagy by interacting raft-like components. ERLIN1 an lipid raft protein prohibitin family. However, little known about its association MAM interface involvement in autophagic initiation. In...
Objective— Systemic Lupus Erythematosus (SLE) and antiphospholipid syndrome (APS) are associated with a high prevalence of atherosclerosis. β2 glycoprotein I (β2GPI) represents link between autoimmunity endothelial dysfunction. Recently, β2GPI reactive T cells have been identified; however, their role in atherosclerosis is still under investigation. We evaluated early patients SLE APS investigated cell reactivity to its relationship atherosclerotic process. Approach Results— Fifty SLE, 18...
In the clinical practice it is possible to find patients with signs suggestive of antiphospholipid syndrome (APS), who are persistently negative for laboratory criteria APS, that is, anti-cardiolipin antibodies (aCL), anti-β2-GPI and lupus anticoagulant. Therefore, was proposed these cases term seronegative APS (SN-APS). order detect autoantibodies different methodological approaches, sera from 24 SN-APS were analysed anti-phospholipid using TLC immunostaining, anti-vimentin/cardiolipin by...
Antiphospholipid syndrome (APS), characterized by artherial and/or venous thrombosis, pregnancy morbidity and “antiphospholipid” antibodies (aPLs), is more common in women than men, with a female to male ratio of about 3.5:1. Only few studies have investigated the clinical differences between patients APS. Therefore, this study was aimed analyze manifestations laboratory tests, at diagnosis, APS outcome. We enrolled 191 consecutive (125 primary APS, PAPS, 66 secondary SAPS) predominant...
Lupus nephritis (LN), caused by immune complexes produced in situ or deposited from the bloodstream, is one of most severe features Systemic Erythematosus (SLE) leading to an increased morbidity and mortality. Toll like receptors (TLRs), such as TLR3, TLR7 TLR9, may play a key role its pathogenesis. Interleukin-32 (IL-32), cytokine involved both innate adaptive responses, has been widely considered autoimmune-inflammatory rheumatic diseases. This study aims evaluate IL-32 LN, also...
Rheumatoid arthritis (RA) and Psoriatic (PsA) are chronic inflammatory diseases mainly affecting joints. RA primarily targets the synovial joints is characterized by cartilage bone erosion, whereas PsA associated with skin nail psoriasis erosive damage an exuberant formation soft tissue involvement. Recent evidence described involvement of Wnt pathway in pathogenesis these diseases. Thus, we aimed to analyze some components signaling, i.e. DKK1, 5a β-catenin, their association disease...
Rheumatoid Arthritis (RA) is a systemic autoimmune disease characterized by chronic synovial inflammation affecting diarthrodial joints, with cartilage destruction and bone erosion. Environmental inflammatory stimuli can induce maturation of dendritic cells (DCs), which promote differentiation activation effector T lymphocytes. We previously highlighted the role extracellular microvesicles (EMVs) in pathogenesis carrying antigens that trigger autoantibody production. In this investigation we...
Autophagy has emerged as a key mechanism in the survival and function of T B lymphocytes, its activation was involved apoptosis resistance rheumatoid arthritis (RA). To investigate whether relationship between autophagy may impact response to therapy, we analyzed ex vivo spontaneous patients with RA subjected treatment anti-tumor necrosis factor (TNF) drugs vitro effects TNFα anti-TNF on cell fate. Peripheral blood mononuclear cells (PBMCs) from 25 treated were for levels marker LC3-II by...
Cardiolipin (CL) is a hallmark phospholipid localized within the inner mitochondrial membrane. Upon several stress conditions, CL translocated to specialized platforms, where it may play role in signaling events promote mitophagy and apoptosis. Recent studies characterized molecular composition of MAM-associated lipid microdomains their implications regulating autophagic process. In this study we analyzed presence MAMs following stimulus possible implication raft-like enriched as platform...
Neuroglobin (NGB) is an O2-binding globin mainly expressed in the central and peripheral nervous systems cerebrospinal fluid. Previously, it was demonstrated that NGB overexpression protects cells from hypoxia-induced death. To investigate processes promoted by overexpression, we used a cellular model of neuroblastoma stably overexpressing NGB-FLAG construct. We proteomic approach to identify specific profile following overexpression. evaluate role increasing energetic metabolism, measured...
We aimed to analyse the prevalence of non-criteria anti-phospholipid (aPL) antibodies and their role in diagnosis, treatment prognosis a cohort patients with clinical features consistent diagnosis antiphospholipid syndrome (APS), but persistently negative for criteria aPL - anti-cardiolipin (aCL), anti-β2-glycoprotein I (aβ2-GPI) lupus anticoagulant (LA) named seronegative APS (SN-APS).Sera from SN-APS were tested aCL by TLC-immunostaining, anti-vimentin/cardiolipin (aVim/CL)...
We have previously demonstrated that human heat shock protein 90 (HSP90), an intracellular self protein, is the target of cellular and humoral autoimmune responses in patients with carotid atherosclerosis. In this study, we evaluated vitro whether oxidative stress, a feature atherosclerotic plaque, alters HSP90 expression endothelial cells, thus inducing surface localization molecule antioxidant compound 7,8‐dihydroxy‐4‐methylcoumarin (7,8‐DHMC) able to prevent stress‐induced alterations...
Antiphospholipid antibody syndrome (APS) is a systemic autoimmune disease characterized by arterial and/or venous thrombosis, pregnancy morbidity in the presence of circulating "anti-phospholipid antibodies" (aPL). One main target antigens aPL β2-glycoprotein I (β2-GPI). APS may occur as primary or associated with Systemic Lupus Erythematosus (SLE). High Mobility Group Box 1 (HMGB1) nuclear non-histone protein which secreted from different type cells during activation cell death and act...
Prion protein (PrPC ) localizes stably in lipid rafts microdomains and is able to recruit downstream signal transduction pathways by the interaction with promiscuous partners. Other proteins have ability occasionally be recruited these specialized membrane areas, within multimolecular complexes. Among these, we highlight presence of low-density lipoprotein receptor-related 1 (LRP1), which was found localized transiently rafts, suggesting a different function this receptor that through raft...