A5022154374- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Eosinophilic Disorders and Syndromes
- Inflammatory Myopathies and Dermatomyositis
- Systemic Sclerosis and Related Diseases
- Advanced Fluorescence Microscopy Techniques
- Dermatologic Treatments and Research
- Cell Image Analysis Techniques
- Connective Tissue Growth Factor Research
- Pain Mechanisms and Treatments
- Hair Growth and Disorders
- Neuroscience of respiration and sleep
- Tactile and Sensory Interactions
- Dermatology and Skin Diseases
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Skin Diseases and Diabetes
- Genetic Neurodegenerative Diseases
- Photonic and Optical Devices
- Cutaneous Melanoma Detection and Management
- IL-33, ST2, and ILC Pathways
- Genital Health and Disease
- Peripheral Neuropathies and Disorders
- Genetic Syndromes and Imprinting
- Cardiovascular Health and Risk Factors
Shandong University of Science and Technology
2025
Duke University
2016-2022
Huashan Hospital
2019-2022
Fudan University
2018-2022
Massachusetts Institute of Technology
2022
McGovern Institute for Brain Research
2022
Zhongshan Hospital
2018-2021
Duke Medical Center
2008-2020
Duke University Hospital
2016-2020
Sun Yat-sen University
2018
In vivo calcium imaging using a 1-photon-based miniscope and microendoscopic lens enables studies of neural activities in freely behaving animals. However, the high fluctuating background, inevitable movements distortions field, extensive spatial overlaps fluorescent signals emitted from imaged neurons inherent this 1-photon method present major challenges for extracting neuronal reliably automatically raw data. Here, we develop unifying algorithm called pipeline (MIN1PIPE), which contains...
Premotor circuits in the brainstem project to pools of orofacial motoneurons execute essential motor action such as licking, chewing, breathing, and rodent, whisking. Previous transsynaptic tracing studies only mapped premotor neonatal mice, but adult remain unknown a consequence technical difficulties. Here, we developed three-step monosynaptic strategy identify neurons controlling vibrissa, tongue protrusion, jaw-closing muscles mouse. We registered these different groups onto Allen mouse...
Low-cost direct modulation detection (DM-DD) systems in optical communications are severely limited by transient chirps when adopting higher-order formats to meet the high-capacity transmission. To break through this constraint, a shaping current waveform technique combined with multi-level pulse amplitude (PAM) is proposed suppress chirp of directly modulated distributed feedback (DFB) lasers. This pre-compensation only requires simply changing injected waveform, which combination phase...
To investigate whether autophagy mediates 2-methoxyestradiol (2-ME)-inhibited hypoxia-induced fibrosis and endothelial-to-mesenchymal transition (endoMT) in SSc.Autophagy the skin of SSc patients was assessed by transmission electron microscopy. fibroblasts human umbilical vein endothelial cells (HUVECs) were cultured under hypoxic (1% O2) conditions with 2-ME or inhibitor. Collagen I connective tissue growth factor (CTGF) vascular (VE)-cadherin, CD31, vimentin α-smooth muscle actin (α-SMA)...
To investigate the mechanism of 2-methoxyestradiol (2-ME) in inhibiting hypoxia-induced collagen synthesis fibroblasts SSc.The expressions hypoxia-inducible factor 1 alpha (HIF-1α) and connective tissue growth (CTGF) skin specimens derived from SSc patients healthy volunteers were examined by immunohistochemistry. HIF-1α was knocked down lentiviral transduction, dermal cultured under normoxic (21% O2) or hypoxic (1% condition treated with PI3K inhibitor LY294002, rapamycin 2-ME (25 μM). The...
Pain relief by vibrotactile touch is a common human experience. Previous neurophysiological investigations of its underlying mechanism in animals focused on spinal circuits, while studies suggested the involvement supraspinal pathways. Here, we examine role primary somatosensory cortex (S1) touch-induced mechanical and heat analgesia. We found that, mice, reafferent signals from self-generated whisking significantly reduce facial nociception, which abolished specifically blocking...
The role of higher-order thalamic structures in sensory processing remains poorly understood. Here, we used the ferret (Mustela putorius furo) as a novel model species for study lateral posterior (LP)-pulvinar complex and its structural functional connectivity with area 17 [primary visual cortex (V1)]. We found reciprocal anatomical connections between part LP nucleus LP-pulvinar (LPl) V1. In order to investigate this feedback loop LPl V1 shaping network activity, determined interactions...
Abstract Objectives DM-related acute/subacute interstitial lung disease (A/S-ILD) remains a big therapeutic challenge due to its insidious onset and rapid development. In the present study, we aimed investigate association between clinical features of DM ILD. Methods We retrospectively assessed skin manifestations, muscle damage, laboratory tests, concurrent ILD malignancy in 207 patients with analysed high-risk factors for Results patients, 153 had ILD, which 131 chronic (CILD) 22 A/S-ILD....
Abstract Objective To clarify the role of RNA polymerase III A (POLR3A)/type I IFN in pathogenesis SSc. Methods Cytosolic DNA and stimulator genes (STING) pathway skin or serum SSc patients were detected by immunofluorescence, immunohistochemistry western blotting. from human macrophages was transfected to fibroblasts umbilical vein endothelial cells (HUVECs) then markers POLR3A/STING real-time qPCR, blotting confocal microscopy. After H151 treatment knocking down POLR3A/STING, type...
SUMMARY In vivo calcium imaging using 1-photon based miniscope and microendoscopic lens enables studies of neural activities in freely behaving animals. However, the high fluctuating background, inevitable movements distortions field, extensive spatial overlaps fluorescent signals emitted from imaged neurons inherent this method present major challenges for extracting neuronal reliably automatically raw data. Here we develop a unifying algorithm called MINiscope PIPEline (MIN1PIPE) that...
Miniscope calcium imaging is increasingly being used to monitor large populations of neuronal activities in freely behaving animals. However, due the high background and low signal-to-noise ratio single-photon based this technique, extraction neural signals from numbers imaged cells automatically has remained challenging. Here we describe a highly accurate framework for identifying activated neurons extracting miniscope data, seeds cleansing Constrained Nonnegative Matrix Factorization...
Objective To analyze clinical and pathological features of dermatomyositis with pan-niculitis as a skin manifestation. Methods Clinical data were collected from 9 cases panniculitis manifestation in Department Dermatology Zhongshan Hospital affiliated to Fudan University October 2012 July 2016, their analyzed. Results Of the cases, 6 female 3 male, age ranged 28 73 years. Panniculitis lesions patients all manifested painful indurated plaques or nodules on buttock, thigh, waist,...
Abstract Premotor circuits in the brainstem control pools of orofacial motoneurons to execute essential functions such as drinking, eating, breathing, and rodent, whisking. Previous transsynaptic tracing studies only mapped premotor neonatal mice but adult remain unknown due technical difficulties. Here we developed a three-step monosynaptic strategy identify neurons controlling whisker, tongue protrusion, jaw-closing muscles adult. We registered these different groups onto Allen mouse brain...