A5069474479- Plasma Applications and Diagnostics
- Protease and Inhibitor Mechanisms
- Plasma Diagnostics and Applications
- Surface Modification and Superhydrophobicity
- Electrohydrodynamics and Fluid Dynamics
- Mass Spectrometry Techniques and Applications
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Congenital Heart Disease Studies
- Cancer, Lipids, and Metabolism
- Cardiac Structural Anomalies and Repair
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Catalytic Processes in Materials Science
- Lipoproteins and Cardiovascular Health
- Extracellular vesicles in disease
- Cellular transport and secretion
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Hypoxia, and Metabolism
- Intraperitoneal and Appendiceal Malignancies
- Cardiac Arrhythmias and Treatments
- Dust and Plasma Wave Phenomena
- S100 Proteins and Annexins
- Legume Nitrogen Fixing Symbiosis
- Cardiac Valve Diseases and Treatments
Nagoya University
2015-2024
Osaka City General Hospital
2014-2024
University of Michigan
1997-2012
Nanjing University
2010
Brigham and Women's Hospital
2006-2010
Kyung Hee University
2010
Yanbian University Hospital
2010
Harvard University
2006-2010
Nanjing Medical University
2010
Kyung Hee University Dental Hospital
2010
Nonequilibrium atmospheric pressure plasma (NEAPP) therapy has recently been focused on as a novel medical practice. Using cells with acquired paclitaxel/cisplatin resistance, we elucidated effects of indirect NEAPP-activated medium (NEAPP-AM) exposure cell viability and tumor growth in vitro vivo.Using chronic paclitaxel/cisplatin-resistant ovarian cancer cells, applied NEAPP-exposed to xenografted tumors mouse model. Furthermore, examined the role reactive oxygen species (ROS) or their...
Glioblastoma brain tumor cells and normal astrocytes were treated with plasma-activated medium (PAM). Cell proliferation assays showed that glioblastoma selectively killed by PAM. PAM induced morphological changes consistent apoptosis in the decreased size. We confirmed those using an apoptotic molecular marker, cleaved Caspase3/7. To elucidate mechanisms of PAM-mediated cells, we investigated effects survival signal transduction pathways. found downregulated expression AKT kinase, a marker...
Two independent ovarian cancer cell lines and fibroblast controls were treated with nonequilibrium atmospheric pressure plasma (NEAPP). Most cells detached from the culture dish by continuous treatment to a single spot on dish. Next, source was applied over whole using robot arm. In vitro proliferation assays showed that treatments significantly decreased rates of compared cells. Flow cytometry western blot analysis induced apoptosis. NEAPP could be promising tool for therapy cancers.
Abstract Non-thermal atmospheric pressure plasma is a novel approach for wound healing, blood coagulation, and cancer therapy. A recent discovery in the field of medicine that non-thermal not only directly but also indirectly affects cells via plasma-treated liquids. This has led to use as chemotherapy. We refer these liquids plasma-activated chose Ringer’s solutions produce clinical applications. In vitro vivo experiments demonstrated lactate solution anti-tumor effects, four components...
Vacuolar matrix proteins in plant cells are sorted from the secretory pathway to vacuoles at Golgi apparatus. Previously, we reported that NH2-terminal propeptide (NTPP) of sporamin precursor and COOH-terminal (CTPP) barley lectin contain information for vacuolar sorting. To analyze whether these propeptides interchangeable, expressed constructs consisting wild-type or mutated NTPP with mature part CTPP tobacco BY-2 cells. The localization indicated signals were interchangeable. We next...
Objective— Although it has been reported that matrix metalloproteinase (MMP)-2 is a major proteinase in atherosclerotic plaque lesions, there no direct evidence of the role MMP-2 lesion formation. In present study we determined atherosclerosis development using apolipoprotein E-deficient (apoE −/− ) mice. Methods and Results— To generate MMP-2–deficient, apoE-deficient mice (MMP-2 :apoE ), were crossed with apoE After 8 weeks feeding lipid-rich diet, morphological biochemical studies aortic...
Non-thermal atmospheric pressure plasma has been proposed as a new therapeutic tool for cancer treatment. Recently, plasma-activated medium (PAM) widely studied in various types. However, there are only few reports demonstrating the anti-tumour effects of PAM an animal model reflecting pathological conditions and accompanying mechanism. Here we investigated inhibitory effect on metastasis ovarian ES2 cells vitro vivo. We demonstrated that cell migration, invasion adhesion were suppressed by...
Ovarian clear cell carcinoma (CCC) is a histological type of epithelial ovarian cancer that less responsive to chemotherapy and associated with poorer prognosis than serous endometrioid carcinoma. Non-thermal atmospheric pressure plasma which produces reactive species has recently led an explosion research in medicine. Plasma treatment can be applied induce apoptosis tumor growth arrest. Furthermore, recent studies have shown medium exposed also anti-proliferative effect against the absence...
Non-equilibrium atmospheric pressure plasma (NEAPP) has attracted attention in cancer therapy. We explored the indirect effect of NEAPP through plasma-activated medium (PAM) on pancreatic cells vitro and vivo. In this study, four cell lines were used antitumor effects PAM treatment evaluated using a proliferation assay. To explore functional mechanisms, morphological change caspase-3/7 activation also assessed. Furthermore, reactive oxygen species (ROS) generation was examined N-acetyl...
We have been developing novel ultrahigh density atmospheric pressure plasma sources and succeeded in the selective killing ovarian cancer cells against normal ones. Furthermore, we found out plasma-activated medium (PAM) also killed glioblastoma brain tumor selectively ones chemical products PAM long lifetime healing effects. To clarify mechanism, interactions of with organism where belongs were investigated on viewpoint intracellular molecular mechanism.
Non-thermal plasma (NTP), an engineered technology to generate reactive species, induces ferroptosis and/or apoptosis specifically in various-type cancer cells. NTP-activated Ringer's lactate (PAL) is another modality for therapy at preclinical stage. Here we found that PAL selective of malignant mesothelioma (MM) cells, where non-targeted metabolome screening identified upregulated citrulline-nitric oxide (.NO) cycle as a target. .NO probe detected biphasic peaks transiently exposure with...
Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sEVs) medium/large (m/lEVs) from cell lines or patient serum ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had proteomic characteristics. Multivalidation steps identified FRα,...
Reactive species are involved in various aspects of neoplastic diseases, including carcinogenesis, cancer-specific metabolism and therapeutics. Non-thermal plasma (NTP) can directly provide reactive species, by integrating atmospheric interjacent molecules as substrates, to represent a handy strategy load oxidative stress situ. NTP causes apoptosis and/or ferroptosis specifically cancer cells types. Plasma-activated Ringer's lactate (PAL) is another modality at the preclinical stage...
Objective— The development of a murine model atherosclerotic plaque rupture. Methods and Results— left common carotid arteries male apolipoprotein E (apoE)–deficient mice (9 weeks old) were ligated just proximal to their bifurcations. After 4 on standard diet, the received polyethylene cuff placement site, animals then processed for morphological studies at specific time points. Ligation artery in apoE-deficient induced marked intimal hyperplasia, which is lipid- collagen-rich lesion that...
Background— Exercise stimulates the vascular response in pathological conditions, including ischemia; however, molecular mechanisms by which exercise improves impaired hypoxia-induced factor (HIF)-1α–mediated to hypoxia associated with aging are poorly understood. Here, we report that swimming training (ST) modulates ischemia aged (24-month-old) mice. Methods and Results— Aged wild-type mice (MMP-2 +/+ ) maintained ST (swimming 1 h/d) from day after surgery were randomly assigned 4 groups...
A plasma-activated medium (PAM), which means a cell-culture irradiated with cold atmospheric plasmas or non-equilibrium pressure plasma (NEAPP), has shown strong antitumor effects on various kinds of cells such as gastric cancer cells, human lung adenocarcinoma breast and so on. In order to clarify the mechanism, it is extremely important investigate behaviors stable unstable reactive oxygen nitrogen species in culture by NEAPP. The roles hydroxyl radicals (•OH) nitric oxide (•NO) were...
We previously reported that plasma-activated medium (PAM) selectively kills glioblastoma brain tumor cells by downregulating the signaling molecule, serine-threonine kinase AKT. AKT plays a key role in survival and proliferation acting as hub molecule network to inhibit apoptosis. The pathways contain are affected PAM unclear. In this study of cells, phosphorylation at both Ser473 Thr308 was downregulated PAM, suggesting upstream mammalian target rapamycin complex 2 (mTORC2)...
Abstract Non-thermal atmospheric pressure plasma has been widely used for preclinical studies in areas such as wound healing, blood coagulation, and cancer therapy. We previously developed plasma-activated medium (PAM) Ringer’s lactate solutions (PAL) treatments. Many vitro vivo experiments demonstrated that both PAM PAL exhibit anti-tumor effects several types of cells ovarian, gastric, pancreatic well glioblastoma cells. However, interestingly, induces more intracellular reactive oxygen...
Peritoneal dissemination of ovarian cancer (OvCa) arises from the surface peritoneum, covered by monolayer mesothelial cells (MCs). Given that both OvCa and MCs are present in same peritoneal metastatic microenvironment, they may establish cell-to-cell crosstalk or phenotypic alterations including acquisition platinum-resistance cells. Herein, we report how OvCa-associated (OCAMs) induce through direct crosstalk. We evaluated mutual associations between human primary with vitro coculturing...