A5045769885- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Photonic Crystals and Applications
- Photonic and Optical Devices
- Semiconductor Lasers and Optical Devices
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Bladder and Urothelial Cancer Treatments
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Animal Nutrition and Physiology
- Chemical Synthesis and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Optical Coatings and Gratings
- TGF-β signaling in diseases
- Carbohydrate Chemistry and Synthesis
- Chemical Reactions and Isotopes
- Animal health and immunology
- Amino Acid Enzymes and Metabolism
- Reproductive biology and impacts on aquatic species
- Renal cell carcinoma treatment
- Chemical Synthesis and Reactions
- Molecular Biology Techniques and Applications
Taiwan Livestock Research Institute
2015-2024
National Yang Ming Chiao Tung University
2019-2024
Council of Agriculture
2012-2022
National Cheng Kung University
2008-2022
Southern Taiwan University of Science and Technology
2010-2018
Inflammation Research Foundation
2009
Mount Sinai Medical Center
2008
Massachusetts Institute of Technology
1999
University of California, Davis
1997
Scripps Research Institute
1995
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of dihydroxyacetone phosphate-dependent aldolases in the synthesis deoxy aza sugarsKevin K. C. Liu, Tetsuya Kajimoto, Lihren Chen, Ziyang Zhong, Yoshitaka Ichikawa, and Chi Huey WongCite this: J. Org. Chem. 1991, 56, 22, 6280–6289Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/jo00022a013https://doi.org/10.1021/jo00022a013research-articleACS...
Embryonic germ (EG) cells are undifferentiated stem isolated from cultured primordial (PGC). To date, EG have been only in the mouse. Murine share several characteristics with embryonic (ES) cells, including morphology, pluripotency, and capacity for germline transmission. We report here isolation of porcine cells. PGC collected Day 24 or 25 embryos were on mitotically inactivated murine fibroblasts. Four cell lines repeated subculture PGC. Porcine morphologically resembled ES consistently...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Evaluation of Homoaza Sugars as Glycosidase InhibitorsChi-Huey Wong, Louis Provencher, John A. Porco Jr., Sang-Hun Jung, Yi-Fong Wang, Lihren Chen, Ruo Darryl H. SteensmaCite this: J. Org. Chem. 1995, 60, 6, 1492–1501Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/jo00111a007https://doi.org/10.1021/jo00111a007research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRecombinant 2-Deoxyribose-5-phosphate Aldolase in Organic Synthesis: Use of Sequential Two-Substrate and Three-Substrate Aldol ReactionsChi-Huey Wong, Eduardo Garcia-Junceda, Lihren Chen, Olga Blanco, Harrie J. M. Gijsen, Darryl H. SteensmaCite this: Am. Chem. Soc. 1995, 117, 12, 3333–3339Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDeoxyribose 5-phosphate aldolase as a catalyst in asymmetric aldol condensationLihren Chen, David P. Dumas, and Chi Huey WongCite this: J. Am. Chem. Soc. 1992, 114, 2, 741–748Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January 1992https://pubs.acs.org/doi/10.1021/ja00028a050https://doi.org/10.1021/ja00028a050research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPalladium-mediated stereocontrolled reductive amination of azido sugars prepared from enzymic aldol condensation: a general approach to the synthesis deoxy aza sugarsTetsuya Kajimoto, Lihren Chen, Kevin K. C. Liu, and Chi Huey WongCite this: J. Am. Chem. Soc. 1991, 113, 17, 6678–6680Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August...
The optimization of a class indole cPLA 2 alpha inhibitors is described herein. importance the substituent at C3 and substitution pattern phenylmethane sulfonamide region are highlighted. Optimization these regions led to discovery 111 (efipladib) 121 (WAY-196025), which shown be potent, selective in variety isolated enzyme assays, cell based rat human whole blood assays. binding compounds has been further examined using isothermal titration calorimetry. Finally, have efficacy when dosed...
The synthesis and structure-activity relationship of a series indole inhibitors cytosolic phospholipase A2alpha (cPLA2alpha, type IVA phospholipase) are described. Inhibitors cPLA2alpha predicted to be efficacious in treating asthma as well the signs symptoms osteoarthritis, rheumatoid arthritis, pain. introduction benzyl sulfonamide substituent at C2 was found impart improved potency these inhibitors, SAR analogues is disclosed. Compound 123 (Ecopladib) sub-micromolar inhibitor GLU micelle...
Abstract Ensuring boar sperm quality before insemination is crucial for maximizing field fertility and efficient pig production. The computer-assisted analysis (CASA) fluorescence probes combined with flow cytometry (FC) are commonly used techniques evaluating kinematics functions, closely related to animal fertility. However, their high cost complex operations make it challenging apply them in laboratories or breeding farms limited resources. Here, our aim was develop a new protocol using...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzymes in organic synthesis: synthesis of highly enantiomerically pure 1,2-epoxy aldehydes, epoxy alcohols, thiirane, aziridine, and glyceraldehyde 3-phosphateRichard L. Pederson, Kevin K. C. Liu, James F. Rutan, Lihren Chen, Chi Huey WongCite this: J. Org. Chem. 1990, 55, 16, 4897–4901Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August...
Apoptosis occurs frequently for blastocysts cultured in vitro, where conditions are suboptimal to those found the natural environment. Insulin-like growth factor-1 (IGF-1) plays an important role preventing apoptosis early development of embryo, as well progressive regulation organ development. We hypothesize that IGF-1 and its dephosphorylated binding protein (IGFBP-1) may be able improve embryo culture with associated reduced cell death, resultant increase total number could chances...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA New Route to Deoxythio sugars Based on AldolasesWen-Chih Chou, Lihren Chen, Jim-Min Fang, and Chi-Huey WongCite this: J. Am. Chem. Soc. 1994, 116, 14, 6191–6194Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://doi.org/10.1021/ja00093a018RIGHTS & PERMISSIONSArticle Views282Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text...
Purpose: Urinary bladder urothelial carcinoma (UBUC) is a common malignant disease in developed countries. Cell-cycle dysregulation resulting uncontrolled cell proliferation has been associated with UBUC development. This study aimed to explore the roles of TMCO1 UBUCs.Experimental Design: Data mining, branched DNA assay, immunohistochemistry, xenograft, culture, quantitative RT-PCR, immunoblotting, stable and transient transfection, lentivirus production knockdown, cell-cycle, viability...
11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD1) is the enzyme that converts cortisone to cortisol. Evidence suggests selective inhibition of 11beta-HSD1 could treat diabetes and metabolic syndrome. Presented herein are synthesis, structure-activity relationship, in vivo evaluation piperazine sulfonamides as inhibitors. Through modification our initial lead 5a, we have identified potent inhibitors such 13q 13u with good pharmacokinetic properties.
We attempted to isolate ES cell lines using inner masses from high-quality cloned porcine blastocysts. After being seeded onto feeders, embryos had better (P < 0.05) attachment, outgrowth formation and primary colonization in both 2× 3× aggregated (62.8, 42.6 and12.8% vs. 76.2, 55.2 26.2%, respectively) compared the non-aggregated group (41.6, 23.4 3.9%). Effects of feeder types (STO MEF) serum sources (FBS KSR) on extraction embryo-derived cells were examined. More (17.1%) ntES over Passage...
We optimized the p-side emission device configuration of photonic-crystal surface-emitting laser (PCSEL) to facilitate easier chip process and wafer level testing as well feasibility lasing at shorter wavelength. Typically, in order obtain uniformly distributed current for larger area PCSELs, output is designed through n-side window due low hole mobility thin cladding layer. However, substrate epi-layers have be isolated before test each single die on wafer, which compromised advantage...
Nanosilicate platelets (NSP), the form of natural silicate clay that was exfoliated from montmorillonite (MMT), is widely used as a feed additive for its high non-specific binding capacity with mycotoxins such fumonisin B1 (FB1), and has been evaluated safety biomedical use including cytotoxicity, genotoxicity, lethal dosage (LD). In study, we further examined toxicity on development CD1 mouse embryos to prevent teratogenesis-induced by FB1. vitro cultures, NSP did not disturb quality intact...