A5024672546- Genetic diversity and population structure
- Neurobiology and Insect Physiology Research
- Plant and Fungal Species Descriptions
- TGF-β signaling in diseases
- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Evolution and Genetic Dynamics
- Plant Pathogens and Fungal Diseases
- Bladder and Urothelial Cancer Treatments
- Nanoparticle-Based Drug Delivery
- Cancer-related molecular mechanisms research
- Olfactory and Sensory Function Studies
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Cellular transport and secretion
- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Nanoplatforms for cancer theranostics
- Cancer-related gene regulation
- Insect Utilization and Effects
- Pacific and Southeast Asian Studies
- Wnt/β-catenin signaling in development and cancer
Ramathibodi Hospital
2016-2025
Mahidol University
2016-2025
Biodiversity Research Center, Academia Sinica
2015
Genomics Research Center, Academia Sinica
2012-2013
Jackson Laboratory
2012
University of Chicago
2007-2008
Institute of Zoology
2008
National Taiwan University
2007-2008
A selective sweep is the result of strong positive selection driving newly occurring or standing genetic variants to fixation, and can dramatically alter pattern distribution allelic diversity in a population. Population-level sequencing data have enabled discoveries sweeps associated with genes involved recent adaptations many species. In contrast, much debate but little evidence addresses whether “selfish” are capable fixation—thereby leaving signatures identical classical sweeps—despite...
Insular species are usually endemic and prone to long-term population reduction, low genetic diversity, inbreeding depression, which results in difficulties conservation. The situation is even more challenging for the glacial relict whose habitats fragmented mountainous regions. Amentotaxus formosana an endangered tree Taiwan. It typically found scattered across different mountain regions, leading discontinuous populations various habitats. Consequently, reproductive isolation may lead deep...
Formalin-fixed paraffin-embedded tissue (FFPET), which is the most widely used pathology archive, usually has low-quality DNA and RNA due to extensive nucleic acid crosslinking. fluorescence in situ hybridization (RNA-FISH) been increasingly utilized research clinical settings diagnose disease pathology. In this study, effect of degradation over archival time on RNA-FISH signals FFPET fresh frozen (FFT) was systematically assessed. RNAscope multiplex fluorescent assay with four...
Salix kusanoi is an endangered riparian tree species endemic to Taiwan. This study aimed evaluate the genetic diversity and population structure across eight fragmented populations employing 33 microsatellite loci. The findings revealed moderate (mean AE = 3.85, HO 0.22) significant deviations from Hardy-Weinberg equilibrium. indicated evolutionary pressure, such as drift inbreeding. Analysis of Molecular Variance (AMOVA) demonstrated evident differentiation among (FST 0.30). Principal...
MicroRNAs (miRNAs) are a class of endogenous, non-coding RNAs that mediate post-transcriptional gene silencing by inhibiting mRNA translation and promoting decay. DICER1, an RNase III endonuclease encoded Dicer1, is required for processing short 21–22 nucleotide miRNAs from longer double-stranded RNA precursors. Here, we investigate the loss Dicer1 in mouse postnatal male germ cells to determine how disruptions miRNA biogenesis pathway may contribute infertility. Reduced levels transcripts...
Drosophila sechellia relies exclusively on the fruits of Morinda citrifolia , which are toxic to most insects, including its sibling species melanogaster and simulans. Although several odorant binding protein ( Obp ) genes olfactory receptor Or have been suggested be associated with D. host shift, a broad view how chemosensory contributed this shift is still lacking. We therefore studied transcriptomes antennae, main organ responsible for detecting food resource oviposition, two species....
Insulin genes in mouse and rat compose a two-gene system which Ins1 was retroposed from the partially processed mRNA of Ins2. When originated how it retained genomes still remain interesting problems. In this study, we used genomic approaches to detect insulin gene copy number variation rodent species investigated evolutionary forces acting on both We characterized phylogenetic distribution new (Ins1) by Southern analyses confirmed sequencing genomes. The results demonstrate that right...
To sense numerous odorants and chemicals, animals have evolved a large number of olfactory receptor genes (Olfrs) in their genome. In particular, the house mouse has ~1,100 Olfr gene family. This makes good model organism to study olfaction-related genes. date, whether male female mice possess same ability detecting environmental is still unknown. Using next generation sequencing technology (paired-end mRNA-seq), we detected 1,088 expressed both epithelium. We found that not only but also...
Abstract Background Antennae of fruit flies are the major organs responsible for detecting environmental volatiles, e.g., egg-laying substrates. An adult antenna contains many sensilla full olfactory sensory neurons, where receptor ( Or ) genes expressed. Each neuron only expresses up to three receptors, making it difficult estimate expression levels by conventional methods. In this study, we applied Illumina RNA sequencing (RNA-seq) study and other in fly antennae. Results from...
Urothelial carcinoma (UC), including upper tract urothelial (UTUC) and urinary bladder (UBUC), is a common malignant disease in developed countries. Oncogenic metabolic lesions have been associated with UC development. Methods: Using data mining, series of studies were performed to study the involvement SLC14A1 specimens, animal models UC-derived cell lines. Results: In two cohorts UTUC (n = 340) UBUC 295), protein level was an independent prognostic factor. Epigenetic silencing contributed...
Although gastric cancer is one of the most common causes death in world, mechanisms underlying this type tumor have not been fully understood. In study, we found that IQGAP3, a member IQGAP gene family, was significantly up-regulated human starting from early stages progression. Overexpression IQGAP3 293T and NIH3T3 cells, which no endogenous expression, resulted morphological change with multiple dendritic-like protrusions enhanced migration. also led to reduced cell–cell adhesion likely as...
Abstract Background The sustainable supply of medicinal plants is important, and cultivating domesticating them has been suggested as an optimal strategy. However, this can lead to a loss genetic diversity. Tripterygium wilfordii Hook. f. plant commonly used in traditional Chinese medicine, but its wild populations are dwindling due excessive harvesting. To protect the species meet increasing demand, it urgent cultivate on large scale. distinguishing between T. hypoglaucum , two similar with...
Recent literature demonstrates that retrogenes tend to leave the X chromosome and integrate onto autosomes evolve male-biased expression patterns. Several selection-based evolutionary mechanisms have been proposed explain this observation. Testing these models requires examining history functional properties of new retrogenes, particularly those show evidence directional movement between (X-related retrogenes). This includes autosomal with parental paralogs on (X-derived retrogenes)...
Pentabromopseudilin (PBrP) is a marine antibiotic isolated from the bacteria Pseudomonas bromoutilis and Alteromonas luteoviolaceus. PBrP exhibits antimicrobial, anti-tumour, phytotoxic activities. In mammalian cells, known to act as reversible allosteric inhibitor of myosin Va (MyoVa). this study, we report that potent transforming growth factor-β (TGF-β) activity. inhibits TGF-β-stimulated Smad2/3 phosphorylation, plasminogen activator inhibitor-1 (PAI-1) protein production blocks...
Waardenburg syndrome (WS) is a prevalent hearing loss syndrome, concomitant with focal skin pigmentation abnormalities, blue iris, and other abnormalities of neural crest-derived cells, including Hirschsprung’s disease. WS clinically genetically heterogeneous it classified into four major types type I, II, III, IV (WS1, WS2, WS3, WS4). WS1 WS3 have the presence dystopia canthorum, while also has upper limb anomalies. WS2 WS4 do not but disease indicates WS4. There more severe subtype...
Abstract Malignant ascites is an abnormal accumulation of fluid within the peritoneal cavity, caused by metastasis several types cancers, including colorectal cancer (CRC). Cancer cells in reflect poor prognosis and serve as a good specimen to study tumour heterogeneity, they represent collection multiple metastatic sites peritoneum. In present study, we have employed single-cell RNA-sequencing (scRNA-seq) explore characterise ascites-derived from CRC patient. The samples were prepared using...