A5053469627- Ion Channels and Receptors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Helicobacter pylori-related gastroenterology studies
- Asthma and respiratory diseases
- Oral health in cancer treatment
- Long-Term Effects of COVID-19
- Gastrointestinal motility and disorders
- Neutropenia and Cancer Infections
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Phagocytosis and Immune Regulation
- Dermatology and Skin Diseases
- Immune Response and Inflammation
- Inflammatory Bowel Disease
- Cancer therapeutics and mechanisms
- Herbal Medicine Research Studies
- Dietary Effects on Health
- Gastric Cancer Management and Outcomes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Analytical Chemistry and Chromatography
- Contact Dermatitis and Allergies
- Parasitic Infections and Diagnostics
- IL-33, ST2, and ILC Pathways
- Biochemical Analysis and Sensing Techniques
National Institute of Biomedical Innovation, Health and Nutrition
2021-2025
Kyoto Pharmaceutical University
2012-2024
Kyoto Institute of Technology
2020
Kyoto Prefectural University of Medicine
2016
Gunma University
2016
Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. carboxypeptidase that degrades angiotensin II, thereby improving the pathologies cardiovascular disease or acute lung injury. Here we show B38-CAP, an ACE2-like enzyme, protective against SARS-CoV-2-induced Endogenous expression downregulated in lungs SARS-CoV-2-infected hamsters, leading to elevation II levels. Recombinant Spike also...
Chemotherapeutic agents, including 5-fluorouracil (5-FU), frequently cause intestinal mucositis resulting in severe diarrhoea and morphological mucosal damage. 5-HT₃ receptor antagonists are clinically effective the treatment of nausea emesis during cancer chemotherapy. Therefore we here have examined effects on 5-FU-induced mice.Intestinal was induced male C57BL/6 mice by daily administration 5-FU (50 mg·kg⁻¹) for 5 days. Effects antagonists, ramosetron (0.01-0.1 ondansetron (5 mg·kg⁻¹),...
Clinical chemotherapy frequently causes intestinal mucositis as a side effect, which is accompanied by severe diarrhea. We recently showed that the cytokine-mediated apoptotic pathway might be important for development of induced 5-fluorouracil (5-FU). Saireito, traditional Japanese herbal (Kampo) medicine, widely used to treat diarrhea and various inflammatory diseases in Japan. In present study, we investigated effect saireito on 5-FU-induced mice, especially relation apoptosis crypt. Male...
5-HT (serotonin) regulates various physiological functions, both directly and via enteric neurons. The present study investigated the role of endogenous 5-HT3 receptors in pathogenic mechanisms involved colonic inflammation, especially relation to substance P (SP) neurokinin-1 (NK1 ) receptor.The effects NK1 receptor antagonists were examined dextran sulphate sodium (DSS)-induced colitis mice. Inflammatory mediator expression distribution also determined.Daily administration ramosetron...
Although NADPH oxidase 1 (NOX1) has been shown to be highly expressed in the gastrointestinal tract, physiological and pathophysiological roles of this enzyme are not yet fully understood. In present study, we investigated role NOX1 pathogenesis intestinal mucositis induced by cancer chemotherapeutic agent 5-fluorouracil (5-FU) mice. Intestinal was Nox1 knockout (Nox1KO) littermate wild-type (WT) mice via single, daily administration 5-FU for 5 days. WT mice, caused severe characterized a...
Background and Purpose The transient receptor potential vanilloid 4 (TRPV4) channel is a non‐selective cation involved in physical sensing various tissue types. present study aimed to elucidate the function expression of TRPV4 channels colonic vascular endothelial cells during dextran sulphate sodium (DSS)‐induced colitis. Experimental Approach role progression inflammation was examined murine DSS‐induced colitis model using immunohistochemical analysis, Western blotting Evans blue dye...
COVID-19, caused by SARS-CoV-2, has spread worldwide with dire consequences. To urgently investigate the pathogenicity of COVID-19 and develop vaccines therapeutics, animal models that are highly susceptible to SARS-CoV-2 infection needed. In present study, we established an model via intratracheal tract in CAG promoter–driven human angiotensin-converting enzyme 2–transgenic (CAG-hACE2) mice. The CAG-hACE2 mice showed several severe symptoms infection, definitive weight loss subsequent...
Transient receptor potential (TRP) vanilloid 1 (TRPV1) and TRP ankyrin (TRPA1), which are non-selective cation channels, play important roles in the sensation of pain. This study investigated TRPV1 TRPA1 dextran sulfate sodium (DSS)-induced murine colitis. DSS (2%) administered for 7 days caused severe colitis that was significantly less TRPV1-deficient (TRPV1KO) TRPA1-deficient (TRPA1KO) mice than wild-type (WT) mice. Similar attenuations were observed TRPV1KO TRPA1KO but not WT had been...
Abstract Taste buds are comprised of taste cells, which classified into types I to IV. Transient receptor potential (TRP) channels play a significant role in perception. TRP vanilloid 4 (TRPV4) is non-selective cation channel that responds mechanical, thermal, and chemical stimuli. The present study aimed define the function expression TRPV4 using Trpv4 -deficient mice. In circumvallate papillae, colocalized with type IV cell epithelial marker but not I, II, or III markers. Behavioural...
Since the outbreak of coronavirus disease 2019 (COVID-19), biomarkers for evaluating severity, as well supportive care to improve clinical course, remain insufficient. We explored potential d-amino acids, rare enantiomers amino assessing severity and protective nutrients against severe viral infections. In mice infected with influenza A virus (IAV) in patients COVID-19 requiring artificial ventilation or extracorporeal membrane oxygenation, blood levels including d-alanine, were reduced...
NOX1/NADPH oxidase, a nonphagocytic isoform of reactive oxygen species–producing enzymes, is highly expressed in the colon, but physiologic and pathophysiologic roles this are not fully understood. The present study investigated role NOX1 development colonic inflammation trinitrobenzene sulfonic acid (TNBS)-induced murine colitis model. Intrarectal injection TNBS caused severe accompanied by body weight loss, diarrhea, increased myeloperoxidase (MPO) activity wild-type (WT) mice. In...
Der p 23 has recently been recognized as a new house dust mite (HDM) major allergen that may be linked to the development of asthma in HDM allergic patients. This study aimed investigate frequency sensitization components including and examine correlation between HDM-sensitization AR symptom score Japanese rhinitis (AR) patients without asthma. Serum samples (n = 120) collected from (12 64 years) were assessed for allergen-specific IgE (s-IgE) by ImmunoCAP (Dermatophagoides pteronyssinus (D....
In this study, we investigated the role of transient receptor potential melastatin 2 (TRPM2), a nonselective cation channel abundantly expressed in inflammatory cells such as macrophages, development postoperative ileus, complication abdominal surgery characterized by gastrointestinal dysmotility. wild-type mice, found that intestinal manipulation, maneuver elicits symptoms typical delays transit fluorescein-labeled dextran, promotes infiltration CD68+ Ly6B.2+ neutrophils, and MPO+ into...
Abstract Background SARS-CoV-2 Omicron variants are highly resistant to vaccine-induced immunity and human monoclonal antibodies. Methods We previously reported that two nanobodies, P17 P86, potently neutralize VOCs. In this study, we modified these nanobodies into trimers, called TP17 TP86 tested their neutralization activities against BA.1 subvariant BA.2 using pseudovirus assays. Next, used nanobody cocktail treat ACE2 transgenic mice infected with lethal dose of strains, original, Delta...
G protein-coupled receptor (GPR) 40 is a for long-chain free fatty acids that enhances glucagon-like peptide (GLP)-2 production in intestinal L-cells. GLP-2 and its analogs have reported to increase remission rates patients with Crohn's disease improve experimental colitis rodents. In the present study, we investigated ameliorative effect of GPR40 activation dextran sulfate sodium (DSS)-induced murine model using specific agonist, AS2034178. The daily administration AS2034178 attenuated...
Pollen from members of the Cupressaceae tree family is one most important causes allergic disease in world. Cryptomeria japonica (Japanese cedar) and Chamaecyparis obtusa cypress) are Japan's common species. The pollen dispersal season mainly February to May. major allergens Japanese cedar cypress exhibit high amino acid sequence similarity due phylogenetic relationship between two An epidemiological study has shown that prevalence pollinosis approximately 40%. Younger children (5 9 years...
Abstract Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. the carboxypeptidase to degrade angiotensin II (Ang II) 1-7 improves pathologies cardiovascular disease acute lung injury. To address whether enzymatic activity protective against COVID-19, we investigated effects B38-CAP, an ACE2-like enzyme, on SARS-CoV-2-induced Expression was significantly downregulated in lungs...
The freezing of water droplets deposited or impinged on cold surfaces causes many problems in traffic lights, power transmission wires and heat exchangers. Thus, the suppression these is very important. In this study, we carried out two-dimensional numerical simulation a horizontal surface using Phase-field method. A new equation was developed to predict an increase total volume due freezing. changes physical properties supercooled with temperature were taken into account for droplet....
Abstract Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as decoy. carboxypeptidase that degrades angiotensin II (Ang II) to 1-7 1-7) thereby improves the pathologies cardiovascular disease or acute lung injury. To address whether activity protective in COVID-19, we investigated effects B38-CAP, an ACE2-like enzyme, on SARS-CoV-2-induced Expression endogenous was significantly downregulated...