A5025370496- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Bone Tissue Engineering Materials
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Connective tissue disorders research
- Atrial Fibrillation Management and Outcomes
- biodegradable polymer synthesis and properties
- Graphene and Nanomaterials Applications
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- Uterine Myomas and Treatments
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Polymer composites and self-healing
- Ethics in Clinical Research
- Cardiovascular Effects of Exercise
- Nanoparticles: synthesis and applications
Medical University of Warsaw
2013-2022
Dana-Farber Cancer Institute
2002-2014
Harvard University
2002-2013
University of Warsaw
2008
Biogen (United States)
2002
Brigham and Women's Hospital
2002
New England Disabled Sports
2002
Tufts University
2002
D-cyclins (cyclins D1, D2, and D3) are components of the core cell cycle machinery. To directly test ability each D-cyclin to drive development various lineages, we generated mice expressing only cyclin or D3. We found that these "single-cyclin" embryos develop normally until late gestation. Our analyses revealed in single-cyclin embryos, tissue-specific expression pattern was lost. Instead, mutant ubiquitously expressed remaining D-cyclin. These findings suggest functions three largely...
Abstract Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. cells remain quiescent and localized between myofiber sarcolemma basal lamina. They are activated in response injury, proliferate, differentiate into myoblasts, recreate myofibers. progenitor support regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level oxygen regulates...
It is expected that use of adult multipotential mesenchymal stem cells (MSCs) for bone tissue engineering (TE) will lead to improvement TE products. Prior clinical application, biocompatibility products need be tested in vitro and vivo. In orthopedic research, sheep are a well-accepted model due similarities with humans assumed predictive human outcomes. this study we uncover differences between ovine marrow-derived MSCs (BMSCs) adipose tissue-derived (ADSCs) response osteogenic media....
Expansion and differentiation of adipose-derived stem cells (ADSCs) in vitro are routinely performed two-dimensional (2D) environments.The study hypothesis was that the utilisation three-dimensional (3D) culture conditions, mimicking natural cell niche, might increase osteogenic commitment ADSCs.Therefore, human ADSCs were seeded 3D systems lacking bioactive material components: spheroids polystyrene scaffolds.Alkaline phosphatase (ALP) activity, a marker early osteogenesis, higher ADSC on...
During meiotic maturation, the majority of oocytes from LT/Sv mice arrest at metaphase I. However, anaphase may be induced through parthenogenetic activation. If this happens within ovary, it often results in development ovarian teratomas. Here, we show that induction first incorrect chromosome segregation. In search molecular basis complex phenotype, analyzed localization/destruction cohesins, as well function components spindle assembly checkpoint (SAC). Both localization and removal...
Resources from biobanks and biorepositories, such as human samples, are of increasing interest to specialists in various fields. However, whilst provide a crucial service, their efficient effective management can prove challenging. When establishing biobank many factors should be considered, the need for appropriate infrastructure, equipment, financial support, highly specialised suitably qualified personnel. The number qualifications necessary personnel depend both on biobank's size type -...
The successful implementation of adipose-derived mesenchymal stem cells (ADSCs) in bone regeneration depends on efficient osteogenic differentiation. However, a literature survey and our own experience demonstrated that current differentiation methods are not effective enough. Since the (MSCs) into osteoblasts adipocytes can be regulated by cyclic adenosine monophosphate (cAMP) signaling, we investigated effects cAMP activator, forskolin, inhibitor, SQ 22,536, early late ADSCs cultured...
For many years optimal treatment for dysfunctional skeletal muscle characterized, example, by impaired or limited regeneration, has been searched. Among the crucial factors enabling its development is finding appropriate source of cells, which could participate in tissue reconstruction serve as an immunomodulating agent (limiting immune response well fibrosis, that is, connective formation), after transplantation to regenerating muscles. MSCs, including those derived from bone marrow, are...
Endometriosis is a common gynecological disorder defined as the presence of endometrial-like tissue (glands and stroma) outside uterus. The etiopathogenesis endometriosis still poorly recognized. It speculated that stage-specific embryonic antigen 1 (SSEA-1)-positive stem-like glandular epithelial cells may contribute to development disease. synthesis SSEA-1 mediated by fucosyltransferase 4 encoded FUT4 gene. Therefore, this study aimed evaluate specific expression mRNA in biopsies...
Despite numerous efforts aiming to characterise glioblastoma pathology (GBM) and discover new therapeutic strategies, GBM remains one of the most challenging tumours treat. Here we propose optimisation in vitro culturing patient-derived cells, namely establishment GBM-derived cultures their maintenance at oxygen tension mimicking oxygenation conditions occurring within tumour. To globally analyse cell states, performed transcriptome analysis cells kept as spheroids serum-free reduced (5%...
Despite the great enthusiasm about tissue engineering during 1980s and many significant basic observations made since then, clinical application of tissue-engineered products has been limited. However, prospect creating new human tissues organs is still exciting continues to be a challenge for scientists clinicians. A arm an extremely complicated biological construction. Considering regrowing requires asking current state-of-the-art real capabilities that it may offer within realistic time...
Abstract The aim of the study was to produce heterophasic graphene nanoplatelets based formulation designed for ink-jet printing and biomedical applications. compositions should meet two conditions: be cytocompatible have rheological properties that allow apply it with technique. In view above conditions, selection suspensions components, such as binder, solvent surfactants performed. first stage research homogeneity dispersion their sedimentation behaviour in diverse solutions were tested....
Event Abstract Back to Activation of Wnt/beta-catenin pathway during osteogenic differentiation adipose derived stem cells in monolayer cultures and 3D spheroids Slawomir Ruminski1, 2, 3, Ilona Kalaszczynska1, 2 Malgorzata Lewandowska-Szumiel1, 1 Medical University Warsaw, 1Department Histology Embryology, Centre for Biostructure Research, Poland Preclinical Research Technology, 3 Postgraduate School Molecular Medicine, Introduction: Wnt/β-catenin activity is essential osteoblast...
Event Abstract Back to Graphene oxide reduction as a result of cells activity Ilona Kalaszczynska1, 2, Mariusz Zdrojek3, Anna Wroblewska3, Jaroslaw Judek3, Slawomir Ruminski1, Magdalena Baran4, Ludwika Lipinska4 and Malgorzata Lewandowska-Szumiel1, 2 1 Medical University Warsaw, Department Histology Embryology, Poland Centre for Preclinical Research Technology, 3 Warsaw Faculty Physics, 4 Institute Electronic Materials (GO) reduced GO (rGO) are attractive materials with unique...