MicroRNAs are 22-24 nt non-coding RNAs that bind to the 3' UTR of target mRNAs, thereby inducing mRNA degradation or inhibiting translation. Due their implication in regulation post-transcriptional processes, role miRNAs hepatocellular carcinoma (HCC) has been extensively studied. However, function miR-7 HCC remains be demonstrated.50 paired tissues and matched peritumor from patients were collected. The level was detected by qRT-PCR. protein Kruppel-like factor 4 (KLF-4) determined western...

The spinal origin of jaundice-induced altered peripheral nociceptive response poorly understood. In the current study, we aimed to first validate rats with bile duct ligation (BDL) as a jaundice model accompanied by response, and then analyze differential gene lncRNA expression patterns in lower thoracic cord on different time courses after BDL operation using high-throughput RNA sequencing. differentially expressed genes (DEGs) identified reverse transcription-quantitative polymerase chain...

Pro-angiogenic factors [vascular endothelial growth factor (VEGF) and basic fibroblast (bFGF)] anti-angiogenic (endostatin) play important roles in the progression of pancreatic cancer. The purpose present study was to investigate knockdown effect by either VEGF or bFGF siRNA on expression secretion endostatin carcinoma cells. Pancreatic cell lines (sw1990, Panc-1 PCT-3) were treated with siRNA. VEGF, determined reverse transcription-polymerase chain reaction (RT-PCR) western blot analysis....

To evaluate the effect of AMD3100 treatment to cholangiocarcinoma by analyzing relationship between them, and provide experimental evidence for whether can become a clinical drug cholangiocarcinoma.Cholangiocarcinoma RBE cell lines were used in this study. MTT proliferation test was evaluating gemcitabine cell. CXCR4, N-cadherin, VEGF-C MMP-9 detect RT-PCR western. Transwell invasion effect.We demonstrated that as concentration increasing from 0.33, 3.33 33.33 uM, survival rate 76.65%,...

Objective To investigate the relationship between CXC chemokine recetper 4 (CXCR4) expression to clinicopathological factors and prognosis in cholangiocarcinoma.Methods The of CXCR4 82 cases cholangiocarcinoma,7 benign bile duct neoplasms 12 non-tumor epithelium was detected by immunohistochemical staining,and that 30 cholangiocarcinoma reverse transcriptase-polymerase chain reaction (RT-PCR).Results positive rate neoplasms,and 75.6％,28.6％ 0,respectively.The significantly higher than (P...

Objective To investigate the relationship between angiostatin and endostatin serum concentrations progression of pancreatic carcinoma.Methods 103 patients with carcinoma were divided into resectable group (n =60) unresectable =43).Normal control =30) was set up.The expression significance these proangiogenic factors respectively analyzed in all groups.Results In normal group,concentrations (35.1 ±5.4) (83.9 ±16.1) μg/L,and those (2.4 ±1.5) (10.1 ± 4.9) nmol/L,respectively.Concentrations...

Objective To explorer the expression of vascular endothelial growth factor C (VEGF-C) and Cyclin D1 in cholangiocarcinoma its relationship with clinicopathological features. Methods Forty-two cases 20 normal tissues were studied. The VEGF-C compared their relationships features analyzed. Results The protein tissues, adjacent bile duct was 83.3%, 7.9% 0.0% respectively. expressions mRNA 0.69±0.11 0.63±0.09, 0.62±0.06 0.58±0.05 0.58±0.06 0.56±0.06 level 530.2±27.8, 497.4±38.0...

Objective The object of this study was to determine the anti-tumor effect lobaplatin in cholangiocarcinoma (CCA) cell line RBE and investigate mechanisms by determines transcription expression levels B lymphoma/leukemia-2 associated X protein (bax),B (bcl-2),Actived-Caspase-3,Caspase-8 Cytochrome C (Cyt-C).Methods cells were cultured vitro,followed various concentration for several times.The inhibition rates determined methyl thiazol tetrazolium (MTT) assay cycle changes detected flow...

Objective To explore the effect of gemcitabine (GEM) in cholangicocarcinoma cells on metastatic potential and epithelial-mesenchymal transition (EMT) through CXC chemokine ligand 12 (CXCL12)/CXC receptor 4 (CXCR4) axis. Methods The vitro model was established using stromal cell derived factor 1 (SDF-1, 100 mg/ml) to activate expression CXCL12/CXCR4 biological axis with a high CXCR4 expression. The proliferation rate measured by methyl thiazol tetrazolium (MTT) method....

Objective To investigate the expression of KL-6 mucin in pancreatic ducatal tissue,and to explore its clinical significance.Methods All specimens form 38 patients who were diagnosed as ductal adenocarcinoma subjected immunohistochemical analysis using monoclonal antibody.Results 100％ found positive staining,and marked staining was 78.9％,remarkable not surrounding noncancer regions tissues (72.3％).KL-6 statistical significance with tumor development and differentiation.Conclusion Most all...

Objective To explore the infiltration of regulatory T cells(Tregs)and its significance in extrahepatic cholangiocarcinoma(EHCC)and to elucidate putative effects cytochrome C oxidase- 2 (COX- 2)expression on Tregs. Methods The forkhead/winged helix transcription factor P3(Foxp3)+ Tregs and expression COX- were studied by immunohistochemistry 94 77 cases EHCC respectively.The correlation between Foxp3+ with clinicopathological features prognosis was analyzed. The influence...

Objective To investigate the possible role of X-box binding protein (XBP1) expression at mRNA level in hepatocarcinogenesis. Methods The XBP1 was determined with RT-PCR method liver carcinoma (42 cases), tissue adjacent to (15 cases) and normal cases). relative obtained by comparing GAPDH level. correlation between clinicopathological features were respectively analyzed. Results results showed that cases (0.4396 ±0.0241) significantly higher tissues (0.4152±0.0252) (0.4095 ±0.0149), (F...

Objective To detect the expression of Cyclin D1 protein and gene in cholangioncarcinoma to elucidate its role. Methods The was detected by flow cytometry ( FCM ) 42 cases 20 normal bile duct, assayed cholangioncarcinoma, 17 tissues adjacent duct reverse transcription-polymerase chain reaction RT-PCR) .The correlation analyzed statistically between mRNA clinicopathological parameters cholangioncarcinoma. Results 0.5845 ± 0.0310,0. 5608 0. 0577, 6288 0942 respectively. higher than others (P...

Objective To detect the express of immunoglobulin-binding protein (Bip) mRNA in tissue hepatoma;demonstrating that it exist activation unfold response (UPR) process growth, and expression UPR was higher than normal tissue. Methods study Bip 42 case example hepatome, 15 paired adjacent hepatic tissues to carcinoma by RT-PCR. qualitation check level Western blotting. Results The hepatoma tissue、adjacent normai respectively 0. 4772 ± 0401、0. 4332 0488、0. 4301 0398; others (P ＜ 05 ). experiment...

Objective To investigate the effects of basic fibroblast growth factor (bFGF) small interfering RNA (siRNA) on expression vascular endothelial (VEGF) in pancreatic carcinoma cells.Methods The and secretion VEGF bFGF cells were inhabited by siRNA siRNA.The was detected using reverse transcription-polymerase chain reaction (RT-PCR) enzyme linked immunosorbent assay (ELISA).Results Both significantly mRNA Panc-1 PCT-3 cells.Both sw1990 cells.Conclusion bFGFsiRNA cells. Key words:...

Objective To detect the expression of proliferating cell nuclear antigen (PCNA) protein and gene in eholangionearcinoma to elucidate its role. Methods The express PCNA was detected by Immunohistochemical technique (SP) 42 cases 20 normal bile duct,and eholangionearcinoma, adjacent duct reverse transcription-polymerase chain reaction (RT-PCR). correlation analysed statistically between mRNA clinicopatho-logical parameters eholangionearcinoma. Results duct,...

Objective To detect the expression of vascular endothelial growth factor (VEGF)-C protein and gene in cholangioncarcinoma to elucidate its role. Methods The VEGF-C was detected by immunohistochemical technique (SP) 42 cases cholangioncarcinomas 20cases normal bile duct, that cholangioncarcinomas, tissues adjacent duct using reverse transcription-polymerase chain reaction (RT-PCR). correlation analysed statistically between mRNA clinicopathological parameters cholangioncarcinoma. Results...