A5084062867- Vector-borne infectious diseases
- Viral Infections and Vectors
- Leptospirosis research and findings
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Geology and Paleoclimatology Research
- Isotope Analysis in Ecology
- Yersinia bacterium, plague, ectoparasites research
- Veterinary medicine and infectious diseases
- Insect symbiosis and bacterial influences
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Marine Biology and Ecology Research
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
- Geochemistry and Elemental Analysis
- Marine and environmental studies
- Dermatological diseases and infestations
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Escherichia coli research studies
- Groundwater and Isotope Geochemistry
- Pharmacological Effects of Natural Compounds
- Zoonotic diseases and public health
Chiba Institute of Science
2014-2023
Nagoya University
1996-2008
University of Shizuoka
1997-2006
Okinawa Prefectural Institute of Health and Environment
2004
National Institute of Infectious Diseases
2004
Shizuoka University
2001-2004
Aichi Medical University
2004
Shizuoka Eiwa Gakuin University
2003
Czech Academy of Sciences, Institute of Microbiology
2002
Shizuoka General Hospital
1995-1997
The causative agents of leptospirosis are responsible for an emerging zoonotic disease worldwide. One the major routes transmission is natural environment contaminated with urine a wide range reservoir animals. Soils and surface waters also host high diversity non-pathogenic Leptospira species which virulence status not clearly established. genus currently divided into 35 classified three phylogenetic clusters, supposedly correlate bacteria. In this study, total 90 strains isolated from...
We have developed a method of graphitization from CO 2 samples for accurate 14 C measurements by accelerator mass spectrometry. Our batch method, using sealed Vycor tube, reduces the risk contamination during and makes it possible to prepare many in short time (typically 20 per day).
Abstract Genetic characteristics of 12 Borrelia isolates from the tick, Ixodes ovatus, I. persulcatus , and rodent, Apodemus speciosus ainu in Japan were compared to members three genospecies burgdorferi sensu lato; B. stricto, garinii group VS461. The methods used this study quantitative microplate DNA hybridization assay restriction fragment length polymorphism (RFLP) analyses flagellin structural genes 16S rRNA genes. six A. identified as using RFLP analysis In contrast, ovatus indicated...
A survey was performed for Lyme disease borrelia in the southern part of China, Zhejiang, Sichuan and Anhui provinces, along Yangtze River valley, May 1997 1998. Twenty isolates from Ixodes granulatus, ovatus, Apodemus agrarius Niviventer confucianus were obtained. These characterized by RFLP 5S-23S rDNA intergenic spacer, sequence analysis 16S flagellin gene, DNA-DNA hybridization analysis, SDS-PAGE Western blotting with mAbs. Six A. agrarius, five I. granulatus collected Zhejiang province...
Abstract We report Anaplasma phagocytophilum infection of Ixodes persulcatus and I. ovatus ticks in Japan. Unique p44/msp2 paralogs (and/or 16S rRNA genes) were detected tick tissues, salivary glands, spleens experimentally infected mice. These findings indicate the public health threat anaplasmosis
Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, status leptospirosis in Philippines regarding remains unknown. A survey was conducted Metro Manila Laguna that analyzed samples obtained from 106 rats. Using microscopic agglutination test, we found 92% rat serum were positive for anti- Leptospira antibodies; common infecting serovars Manilae, Hebdomadis, Losbanos. On basis pulsed-field gel electrophoresis gyrase B gene sequence analyses,...
Since research on Leptospira has focused pathogenic leptospires, which are supposed to multiply only in animal hosts, the life cycle of saprophytic leptospires long been a mystery. This study demonstrates that both and waterlogged soil, mimics postflooding environment.
The tick Ixodes ricinus is responsible for the transmission of a number bacterial, protozoan and viral diseases to humans animals in Europe Northern Africa. Female I. from England, Switzerland Italy have been found harbour an intracellular alpha-proteobacterium, designated IricES1, within cells ovary. IricES1 only prokaryote known exist mitochondria any animal or multicellular organism. To further examine distribution, prevalence mode we performed polymerase chain reaction screening adults...
Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour–Stoenner–Kelly (BSK) II medium; samples were taken Istanbul area in northwestern Turkey [ Güner et al. (2003) . Microbiology 149, 2539–2544]. Here is presented detailed characterization of spirochaete. Electron microscopy revealed that strain IST7T morphologically similar to other spirochaetes genus Borrelia and possesses 15 16 flagellae...
Here, we describe for the first time prevalence and genetic properties of Bartonella organisms in wild rodents Japan. We captured 685 throughout Japan (in 12 prefectures) successfully isolated from 176 (isolation rate, 25.7%). Those isolates were all obtained suburban areas (rate, 51.8%), but no organism was animals city areas. Sequence analysis rpoB gltA revealed that classified into eight groups, comprising closely related to B. grahamii (A-I group), tribocorum elizabethae (B-J...
ABSTRACT Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Most of the outbreaks leptospirosis occur after floods heavy rain in countries where are endemic. It has been believed that overflow seawater rarely causes because leptospires killed salt water. On 8 November 2013, storm surge Super Typhoon Haiyan (Yolanda) inundated entire coastal areas Tacloban and Palo Leyte, Philippines. The present study was carried out order to determine whether environmental soil were able...
ABSTRACT We performed a multicenter evaluation of robust and easily dipstick assay for the serodiagnosis human leptospirosis. The is aimed at detection Leptospira -specific immunoglobulin M (IgM) antibodies. study involved 2,665 serum samples collected from 2,057 patients with suspected leptospirosis in 12 countries on five continents different levels endemicity surveillance systems. were grouped as laboratory-confirmed case noncase based results culturing microscopic agglutination test....
Lyme borreliosis is the most prevalent tick-borne zoonosis and an important emerging infection in Europe, North America, Far Eastern countries. The geographical distribution of Borrelia spp. relationship between tick East Asian countries have been studied. In Northern countries, Ixodes persulcatus carries Eurasian-type garinii (20047 type), afzelii, Asian-type B. (variant NT29), whereas burgdorferi sensu stricto has not isolated. contrast, found European vector ricinus. These cause their...
In order to investigate the presence and prevalence of Lyme borreliosis (Lyme disease) Borrelia species, 312 unfed ticks were collected by flagging at a woodland area in Trakya, European side Turkey, May 2002. Twelve 299 Ixodes ricinus infected with spp., as determined cultivation BSK medium (prevalence rate 4.0 %). Ten pure cultures subjected further characterization sequencing analysis 5S-23S rDNA intergenic spacer, 16S flagellin gene. One isolate burgdorferi sensu stricto, two garinii...
Spirochete strains HP3 and HO14, isolated from Ixodes persulcatus I. ovatus in Hokkaido 1989, were the first isolates of Borrelia burgdorferi, etiological agent Lyme disease, to be recognized Japan. Antigenic properties Japanese compared with those United States (B31 297) Europe (IRS, P/Gau, P/Bi, 2/B45, 3/B56) by Western blotting (immunoblotting), using monoclonal antibodies (MAbs) against B31 P/Bi. The reacted MAb U40 41-kDa antigen. E34a31 Osp A all tested except for strain HP3....
ABSTRACT Multilocus sequence typing of Borrelia garinii isolates from humans and comparison with rodent tick were performed. Fifty-nine divided into two phylogenetic groups, an association was detected between clinical isolates, suggesting that, in Japan, human-pathogenic B. comes rodents via ticks.
Twenty-nine isolates of Lyme borreliosis (LB) group spirochaetes collected from ticks and rodents in China Japan were included a multilocus sequence analysis (MLSA). Using different typing system, three these strains had previously been identified as being divergent other LB spirochaete species the name 'Borrelia yangtze' sp. nov. was proposed. The data presented here confirm that genetic distance, calculated using sequences MLSA housekeeping genes, to known < 95 % Borrelia valaisiana 96.67...
Antibacterial effects in terms of biofilm formation and swarming motility were studied using polyacrylate plates having protruding or recessed shark skin micropatterned surfaces with a shallow groove (2 μm pattern width spacing, 0.4 height). It was found that Pseudomonas aeruginosa strongly inhibited by the (0.4 μm) height. Biofilm Staphylococcus aureus also inhibited. Live bacteria located on rather than spacing. When shape linear ridge instead skin, antibacterial weaker seen pattern. The...