A5033299775- Leptospirosis research and findings
- Viral Infections and Vectors
- Veterinary medicine and infectious diseases
- Antifungal resistance and susceptibility
- Humic Substances and Bio-Organic Studies
- Hepatitis Viruses Studies and Epidemiology
- Hepatitis B Virus Studies
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- HIV/AIDS drug development and treatment
- Mental Health Treatment and Access
- Cardiovascular Health and Risk Factors
- Mycobacterium research and diagnosis
- Transgenic Plants and Applications
- Antimicrobial Resistance in Staphylococcus
- Piperaceae Chemical and Biological Studies
- Advanced Drug Delivery Systems
- Phytochemical compounds biological activities
- Listeria monocytogenes in Food Safety
- Vibrio bacteria research studies
- Hepatitis C virus research
- Ideological and Political Education
- DNA and Nucleic Acid Chemistry
- Diagnosis and treatment of tuberculosis
- Tailings Management and Properties
St. Luke's Medical Center
2020-2024
University of the Philippines Manila
2011-2023
University of the Philippines System
2010-2020
De La Salle University – Dasmariñas
2015
National Institutes of Health
2010
A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 amphotericin B, 5 fosfomycin, 400 and 5-fluorouracil, 100 μg/mL) was developed for selective isolation leptospires from contaminated samples. The growth 16 microorganisms considered as possible contaminants during Leptospira were inhibited by this cocktail. In contrast, the a smaller inoculum (10(1) cells per mL) 25 strains (representing 18 serovars/serogroups species) not suppressed combination. This...
ABSTRACT There have been few reports on the epidemiological analysis of environmental Leptospira isolates. This is probably because isolation leptospires from environment was usually unsuccessful due to overgrowth contaminants and slow growth . In this study, we collected a total 88 samples soil water three sites: Metro Manila Nueva Ecija, Philippines (an area where now endemic), Fukuoka, Japan once endemic). We succeeded in isolating 37 by using novel combination five antimicrobial agents...
Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, status leptospirosis in Philippines regarding remains unknown. A survey was conducted Metro Manila Laguna that analyzed samples obtained from 106 rats. Using microscopic agglutination test, we found 92% rat serum were positive for anti- Leptospira antibodies; common infecting serovars Manilae, Hebdomadis, Losbanos. On basis pulsed-field gel electrophoresis gyrase B gene sequence analyses,...
Since research on Leptospira has focused pathogenic leptospires, which are supposed to multiply only in animal hosts, the life cycle of saprophytic leptospires long been a mystery. This study demonstrates that both and waterlogged soil, mimics postflooding environment.
Strain Eri-1(T) was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology isolate were similar to those members genus Leptospira, but spiral structure sharper under dark-field microscopy. Cells 10.6 ± 1.3 µm long 0.2 in diameter, with wavelength 0.9 an amplitude 0.4 µm. grew Korthof's medium at both 13 30 °C, also presence 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain within radiation Leptospira where it formed...
Summary Weil's disease, the most severe form of leptospirosis, is characterized by jaundice, haemorrhage and renal failure. The mechanisms jaundice caused pathogenic L eptospira remain unclear. We therefore aimed to elucidate integrating histopathological changes with serum biochemical abnormalities during development in a hamster model W eil's disease. In this work, we obtained three‐dimensional images infected livers using scanning electron microscope together freeze‐cracking cross‐cutting...
ABSTRACT Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Most of the outbreaks leptospirosis occur after floods heavy rain in countries where are endemic. It has been believed that overflow seawater rarely causes because leptospires killed salt water. On 8 November 2013, storm surge Super Typhoon Haiyan (Yolanda) inundated entire coastal areas Tacloban and Palo Leyte, Philippines. The present study was carried out order to determine whether environmental soil were able...
Leptospirosis is an infectious disease caused by the spirochete bacteria Leptospira spp. and commonly found throughout world. Diagnosis of leptospirosis performed culture microscopic agglutination tests laborious time-consuming. Therefore, we aimed to develop a novel immunochromatography (ICG)-based method for detecting antigen in urine patients animals. We used 1H6 monoclonal antibody (MAb), which specific lipopolysaccharide (LPS) that common among The MAb was coupled 40-nm-diameter...
Within the past few decades, improvement in sanitation and economic growth has driven a changing epidemiology of hepatitis A Western Pacific Region (WPR) World Health Organization (WHO). In this review, we gathered available published information on countries WPR reviewed trends reported literature from years 2000 to 2021. Many have shifted high endemicity low endemicity. Moreover, administration vaccine among children recent disease susceptibility older population. Seroprevalence decreased...
The in vitro susceptibilities of 46 Leptospira isolates from rats to 14 antimicrobial agents were tested. All the strains found be sensitive ampicillin, cefotaxime, ciprofloxacin, norfloxacin, doxycycline, erythromycin, and streptomycin. In contrast, tested showed resistance amphotericin B, 5-fluorouracil, fosfomycin, trimethoprim, sulfamethoxazole, neomycin, vancomycin. These findings will help selecting effective ineffective antimicrobials for treatment leptospirosis development new...
Leptospirosis is caused by pathogenic species of Leptospira. The aim this study was to determine and characterize the pathogenicity four dominant Leptospira isolates prevailing among rats in Philippines. were interrogans serovar Manilae strain K64, L. Losbanos K37, Ratnapura K5 borgpetersenii Javanica K6. Pathogenicities studied using hamsters, which reproduce severe human leptospirosis. minimum lethal doses 10(0) ( = 1) leptospires for K37 K5, 10(1) Weight loss amongst Leptospira-infected...
Leptospirosis caused by drinking water has not been as frequently reported percutaneous infection. Resistance to oral infection pathogenic Leptospira was examined in an experimental hamster model. The results suggested some natural defenses against Leptospira. First, we found that characteristic linear agglutination of rapidly occurs when mixed with human saliva. That saliva attenuated the infectivity treated leptospires its activity be first line defense leptospires. Second, only 10 1...
Leptospirosis is highly endemic in the Philippines and a serious concern to public health. Local research on candidate vaccine moving through development pipeline. The availability of vaccines alone does not guarantee acceptance because individuals' vaccination choice decision influenced by several factors. This study assessed how attributes socio-demographic factors affect acceptability leptospirosis vaccine; estimated willingness pay for vaccine. A discrete experiment was conducted among...
Leptospirosis is a serious public health concern in the Philippines, not only because of its increasing incidence rate, but also significant and economic impacts. Despite relatively high seroprevalence, knowledge on burden disease, particularly value that society places disease prevention remains limited. Obtaining such information important within context policy. This study was conducted Metro Manila to determine leptospirosis, by asking respondents about their willingness contribute labor...
Coronavirus disease 2019 (COVID-19) continues to devastate the world’s health and economy, affecting all aspects of life leading widespread social disruption. Even as several vaccines have been developed, their availability in developing countries is limited efficacy against variants SARS-CoV-2 (severe acute respiratory syndrome–coronavirus 2) needs be continuously assessed. The World Health Organization (WHO) has acknowledged that alone will not overcome global challenges COVID-19....
Background: One of the most important parameters in a commercial fermented milk drink containing probiotics is viability microorganisms. Aims and Objectives: This study described kinetics Lactobacillus casei Shirota strain during refrigerated storage using nonlinear models. Materials Methods: Viability L. was monitored standard bacterial plate count method. Several mathematical models such as zero-order, first-order, second-order kinetic equations were employed describing population...
Multivariate statistical models were utilized to identify the interaction between inhibitory activity and molecular properties of different antimycotics against Microsporum canis.Information on potency M. canis antifungal agents obtained from literature.The relationship was established using multiple linear regression analysis (MLRA) principal component (PCA).Three major descriptors: topological polar surface area, weight, rotatable bond count identified confer action MLRA (r 2 =0.8968,...
Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The non-specific clinical signs and symptoms of leptospirosis lead to its misdiagnosis. To date, there still no reliable rapid test kit that can accurately diagnose at bedside or in field. In this research, with the ultimate goal formulating accurate diagnostic tool for leptospirosis, we aimed identify leptospiral proteins excreted urine infected hamsters, which are thought mimic Weil's disease. Hamsters were subcutaneously...
Leptospirosis is a worldwide zoonosis. The importance of urban leptospirosis recognized in Japan: rats carry pathogenic leptospires and people acquire these pathogens through contact with surface water or soil contaminated by the urine infected animals. To determine current Leptospira carriage rate rats, 29 wild were trapped central area Fukuoka strains isolated from their kidneys analyzed. When semi-solid Korthof's medium containing 0.1% agar was used for isolation, 72.2% 30.8% kidney...
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