A5017187278- Immune cells in cancer
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Cloud Computing and Resource Management
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Service-Oriented Architecture and Web Services
- Epigenetics and DNA Methylation
- Software System Performance and Reliability
- Retinal Diseases and Treatments
- IoT and GPS-based Vehicle Safety Systems
- IoT and Edge/Fog Computing
- Gut microbiota and health
- Business Process Modeling and Analysis
- Neonatal Respiratory Health Research
- Inflammation biomarkers and pathways
- Vehicular Ad Hoc Networks (VANETs)
- Traffic Prediction and Management Techniques
- Single-cell and spatial transcriptomics
- Respiratory viral infections research
- Sphingolipid Metabolism and Signaling
- Mobile Crowdsensing and Crowdsourcing
- Distributed and Parallel Computing Systems
- Lipid Membrane Structure and Behavior
- Nosocomial Infections in ICU
Amsterdam University Medical Centers
2020-2025
University of Amsterdam
2020-2025
Center for Molecular Medicine and Immunology
2024
Baptist Memorial Hospital
2023
Triangle
2023
Amsterdam UMC Location University of Amsterdam
2020-2022
University of Liverpool
2013-2021
University of Bristol
2020
Intel (Ireland)
2008-2018
Royal Liverpool University Hospital
2015-2017
The heterogeneity in sepsis is held responsible, part, for the lack of precision treatment. Many attempts to identify subtypes patients those with shared underlying biology or outcomes. To date, though, there has been limited effort determine overlap across these previously identified subtypes. We aimed concordance critically ill classified by four described subtype strategies.
The plasma lipidome has the potential to reflect many facets of host status during severe infection. Previous work is limited specific lipid groups or was focused on lipids as prognosticators.
There is limited knowledge on how the source of infection impacts host response to sepsis. We aimed compare in sepsis patients with a single, known at admission (< 24 h) intensive care unit.From molecular diagnosis and risk stratification (MARS) prospective cohort, we measured 16 plasma biomarkers reflective key pathways 621 patients. In subgroup (n = 335), blood leukocyte transcriptomes were compared between sources. Differences clinical patient profiles survival whole cohort 2019).The...
Sepsis is a life-threatening condition arising from an aberrant host response to infection. Recent single-cell RNA sequencing investigations identified immature bone-marrow-derived CD14
Most macrophages generate energy to mount an inflammatory cytokine response by increased glucose metabolism through intracellular glycolysis. Previous studies have suggested that alveolar (AMs), which reside in a glucose-poor natural environment, are less capable utilize glycolysis and instead rely on other substrates fuel oxidative phosphorylation (OXPHOS) for supply. At present, it is not known whether AMs use produce cytokines when metabolic options blocked. Here, we studied human...
The association of ageing with increased sepsis mortality is well established. Nonetheless, current investigations on the influence age host response aberrations are largely limited to plasma cytokine levels while neglecting other pathophysiological domains like endothelial cell activation and function, coagulation activation. primary objective this study was gain insight into in key pathways blood transcriptomes sepsis.We analysed clinical outcome (n = 1952), 16 biomarkers providing...
Introduction Immune response dysregulation has been implicated in the development of intensive care unit (ICU)-acquired pneumonia. We aimed to determine differences longitudinal blood transcriptional between patients who develop ICU-acquired pneumonia (cases) and those do not (controls). Methods performed a case-cohort study mechanically ventilated trauma surgery with ICU stays >2 days, enrolled 30 hospitals across Europe. collected for RNA sequencing at baseline, day 7 (in cases)...
The application of sepsis subtypes to enhance personalized medicine in critically ill patients is hindered by the lack validation across diverse cohorts and absence a simple classification model. We aimed validate previously identified SENECA clinical multiple large ICU cohorts, develop parsimonious classifier models for δ-type adjudication practice. Data from four between 2008 2023 were used assign α, β, γ fulfilling Sepsis-3 criteria using variables: (I) Molecular diAgnosis Risk...
Objectives/Goals: This study tests the REDCap Clinical Data Interoperability Service (CDIS) for streamlined data extraction from electronic health records (EHRs) research. Managed by and Translational Science Institute, IU Health, Eskenazi CDIS offers real-time syncing, automated workflows, HIPAA-compliant security. Methods/Study Population: The uses Fast Health Resource (FHIR) Application Programming Interface (API) to extract EHRs. It includes Pull (CDP), which automatically pulls EHR into...
Abstract Ageing presents adverse effects on the retina and is primary risk factor for age‐related macular degeneration (AMD). We report first RNA‐seq analysis of transcriptional changes in human retinal pigment epithelium (RPE), site AMD pathogenesis. Whole transcriptome sequencing RPE from donors ranging age 31 to 93 reveals that ageing associated with increasing transcription main RPE‐associated visual cycle genes (including LRAT , RPE65 RDH5, RDH10 RDH11 ; pathway enrichment BH‐adjusted P...
Age-related macular degeneration (AMD) and Alzheimer's disease (AD) are degenerative, multifactorial diseases involving age-related accumulation of extracellular deposits linked to dysregulation protein homeostasis. Here, we strengthen the evidence that an nsSNP (p.Ala25Thr) in cysteine proteinase inhibitor cystatin C gene CST3, previously confirmed by meta-analysis be associated with AD, is exudative AMD. To our knowledge, this first report highlighting a genetic variant increases risk...
Circulating nonadherent monocytes can migrate to extravascular sites by a process that involves adherence. Alterations in intracellular metabolism shape the immunological phenotype of phagocytes upon activation. To determine effect adherence on their metabolic and functional response human were stimulated with LPS under adherent conditions. Adherent (relative monocytes) produced less TNF IL-1β (proinflammatory) more IL-10 (anti-inflammatory) stimulation had an increased capacity phagocytose...
The plasticity of monocytes enables them to exert multiple roles during an immune response, including promoting tolerance. How alter their functions convey tolerance in the context lower respiratory tract infections humans is not well understood. Here, we sought identify epigenetic and transcriptomic features cytokine production capacity circulating community-acquired pneumonia (CAP).
CCAAT/enhancer-binding protein delta (C/EBPδ) is a member of the C/EBP family transcription factors. According to current paradigm, C/EBPδ potentiates cytokine production and modulates macrophage function thereby enhancing inflammatory response. Remarkably, however, deficiency does not consistently lead reduction in Lipopolysaccharide (LPS)-induced by macrophages. Here, we address this apparent discrepancy show that effect on depends both subtype LPS concentration used. Using CRISPR-Cas...
Future Internet (FI) research and development threads have recently been gaining momentum all over the world as such international race to create a new generation is in full swing: GENI, Asia Internet, Forum Korea, European Union Assembly (FIA). This position paper identifying orientation with time horizon of 10 years, together key challenges for capabilities Management Service-aware Networking Architectures (MANA) part allowing parallel federated Internet(s).
Human polymorphisms with derived alleles that are protective against disease may provide powerful translational opportunities. Here we report a method to identify such candidate and apply it common non-synonymous SNPs (nsSNPs) associated diseases. Our study also sought establish which of the identified nsSNPs show evidence positive selection, taking this as indirect variant has beneficial effect on phenotype. Further, performed an analysis quantify predicted each protein function/structure....
Abstract Ixodes ricinus is the vector for Borrelia afzelii , predominant cause of Lyme borreliosis in Europe, whereas scapularis burgdorferi USA. Transcription several I. genes changes presence B. and contributes to successful infection. To what extend influences gene expression salivary glands largely unknown. Therefore, we measured uninfected vs. infected tick gland during feeding using Massive Analysis cDNA Ends (MACE) RNAseq, quantifying 26.179 unique transcripts. While was main...
Neutrophils are potent immune cells with key antimicrobial functions. Previous in vitro work has shown that neutrophil effector functions mainly fueled by intracellular glycolysis. Little is known about the state of neutrophils still circulation patients during infection. Here, we combined flow cytometry, stimulation assays, transcriptomics, and metabolomics to investigate link between inflammatory metabolic pathways blood community-acquired pneumonia. Patients' neutrophils, relative from...
The lipidome of immune cells during infection has remained unexplored, although evidence the importance lipids in context immunity is mounting. In this study, we performed untargeted lipidomic analysis blood monocytes and neutrophils from patients hospitalized for pneumonia age- sex-matched noninfectious control volunteers. We annotated 521 706 neutrophils, respectively, which were normalized to an extensive set internal standards per lipid class. cellular lipidomes profoundly altered...
Airway epithelial cells represent the first line of defense against respiratory pathogens. Flagellin drives motility many mucosal pathogens and has been suggested as an immune enhancing adjunctive therapeutic in infections airways. This study leveraged single-cell RNA sequencing to determine cell-specific effects flagellin primary human bronchial growing air-liquid interface. Seven cell clusters were identified, including ciliated cells, ionocytes, several states basal secretory which only...
Mesenchymal stem cells (MSC) have immune regulatory properties that may ameliorate pathophysiological processes in sepsis. We determined the effect of allogeneic adipose-derived MSCs (Cx611) on host response during sepsis due to community-acquired bacterial pneumonia (CABP) by measuring 29 plasma biomarkers and blood transcriptomes at six time points 82 patients randomised two intravenous infusions Cx611 or placebo. treatment enhanced several endothelial cell procoagulant biomarkers, led...