Justin de Brabander
A5066802482- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Sepsis Diagnosis and Treatment
- Respiratory viral infections research
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Diabetes and associated disorders
- Mechanical Circulatory Support Devices
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Biomarkers in Disease Prognosis
- Thermal Regulation in Medicine
- Immune Response and Inflammation
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Antifungal resistance and susceptibility
- Pancreatitis Pathology and Treatment
- Tryptophan and brain disorders
- Cardiac Arrest and Resuscitation
- Immunodeficiency and Autoimmune Disorders
- Pharmaceutical Practices and Patient Outcomes
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Nosocomial Infections in ICU
- Patient Satisfaction in Healthcare
Amsterdam University Medical Centers
2018-2025
University of Amsterdam
2018-2025
Leidos (United States)
2024
Leidos Biomedical Research Inc. (United States)
2024
Frederick National Laboratory for Cancer Research
2024
Center for Molecular Medicine and Immunology
2022-2023
Vrije Universiteit Amsterdam
2023
Eijkman Institute for Molecular Biology
2021-2022
Oxford University Clinical Research Unit Indonesia
2021-2022
ASST Fatebenefratelli Sacco
2022
Immunoglobulin G (IgG) antibodies are crucial for protection against invading pathogens. A highly conserved N-linked glycan within the IgG-Fc tail, which is essential IgG function, shows variable composition in humans. Afucosylated variants already used anticancer therapeutic their increased activity through Fc receptors (FcγRIIIa). Here, we report that afucosylated (approximately 6% of total humans) specifically formed enveloped viruses but generally not other antigens. This mediates...
BackgroundMortality rates are high among hospitalized patients with COVID-19, especially in those intubated on the ICU. Insight pathways associated unfavourable outcome may lead to new treatment strategies.MethodsWe performed a prospective cohort study of COVID-19 admitted general ward or ICU who underwent serial blood sampling. To provide insight involved disease progression, associations were estimated between risk and measurements 64 biomarkers potential important infection (inflammation,...
Rationale: Bacterial lung microbiota are correlated with inflammation and acute respiratory distress syndrome (ARDS) altered in severe coronavirus disease (COVID-19). However, the association between (including fungi) resolution of ARDS COVID-19 remains unclear. We hypothesized that increased bacterial fungal burdens related to nonresolving mortality COVID-19. Objectives: To determine relation clinical outcomes COVID-19–related ARDS. Methods: This observational cohort study enrolled...
Patients with COVID-19-related acute respiratory distress syndrome (ARDS) show limited systemic hyperinflammation, but immunomodulatory treatments are effective. Little is known about the inflammatory response in lungs and if this could be targeted using high-dose steroids (HDS). We aimed to characterise alveolar immune patients ARDS, determine its association mortality, explore between HDS treatment response.In observational cohort study, a comprehensive panel of 63 biomarkers was measured...
Abstract Autoantibodies (auto-Abs) neutralizing type I interferons (IFNs) are found in the blood of at least 15% unvaccinated patients with life-threatening COVID-19 pneumonia. We report here presence auto-Abs IFNs bronchoalveolar lavage (BAL) 54 415 (13%) pneumonia tested. The individuals BAL included 45 (11%) against IFN-α2, 37 (9%) IFN-ω, IFN-α2 and/or ω, and five (1%) IFN-β, including three (0.7%) two (0.5%) IFN-β. Auto-Abs also neutralize other 12 subtypes IFN-α. Paired plasma samples...
BackgroundPost-acute sequela of SARS-CoV-2 infection (PASC) encompass fatigue, post-exertional malaise and cognitive problems. The abundant expression the tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase-2 (IDO2) in fatal/severe COVID-19, led us to determine, an exploratory observational study, whether IDO2 is expressed active PASC, may correlate with pathophysiology.MethodsPlasma or serum, peripheral blood mononuclear cells (PBMC) were obtained from well-characterized PASC...
Human herpesviruses, particularly cytomegalovirus (CMV) and herpes simplex virus (HSV), frequently reactivate in critically ill patients, including those with acute respiratory distress syndrome (ARDS) related to coronavirus disease 2019 (COVID-19). The clinical interpretation of pulmonary herpesvirus reactivation is challenging there ongoing debate about its association mortality benefit antiviral medication. We aimed quantify the incidence pathogenicity CMV HSV reactivations COVID-19 patients.
Mortality in cardiogenic shock patients remains high. Short-term mechanical circulatory support with Impella can be used to the circulation these patients, but data from randomised controlled studies and 'real-world' are sparse. The aim is describe real-life on outcomes complications of our 12 years clinical experience after acute myocardial infarction identify predictors 6-month mortality.We a single-centre registry October 2004 December 2016 including all treated for infarction. We report...
<h3>Importance</h3> It is unclear when, where, and by whom health care workers (HCWs) working in hospitals are infected with SARS-CoV-2. <h3>Objective</h3> To determine how often what manner nosocomial SARS-CoV-2 infection occurs HCW groups varying exposure to patients COVID-19. <h3>Design, Setting, Participants</h3> This cohort study comprised 4 weekly measurements of SARS-CoV-2–specific antibodies collection questionnaires from March 23 June 25, 2020, combined phylogenetic epidemiologic...
Background Imatinib reduced 90-day mortality in hospitalised coronavirus disease 2019 (COVID-19) patients a recent clinical trial, but the biological effects that cause improved outcomes are unknown. We aimed to determine changes elicited by imatinib with COVID-19 and what baseline profile moderates effect of imatinib. Methods undertook secondary analysis randomised, double-blind, placebo-controlled trial oral hospitalised, hypoxaemic patients. Mediating plasma concentration 25 host response...
Afucosylated IgG1 responses have only been found against membrane-embedded epitopes, including anti-S in SARS-CoV-2 infections. These responses, intrinsically protective through enhanced FcγRIIIa binding, can also trigger exacerbated pro-inflammatory severe COVID-19. We investigated if the BNT162b2 mRNA induced afucosylated IgG responses.Blood from vaccinees during first vaccination wave was collected. Liquid chromatography-Mass spectrometry (LC-MS) used to study Fc glycoprofiles....
Background Coronavirus disease 2019 (COVID-19)-induced mortality occurs predominantly in older patients. Several immunomodulating therapies seem less beneficial these The biological substrate behind observations is unknown. aim of this study was to obtain insight into the association between ageing, host response and patients with COVID-19. Methods We determined 43 biomarkers reflective alterations four pathophysiological domains: endothelial cell coagulation activation, inflammation organ...
IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both influenced by the composition conserved N-linked glycan located in domain. Here, we compared anti-Spike (S) IgG1 glycosylation profiles response to mRNA, adenoviral, protein-based COVID-19 vaccines mass spectrometry (MS). All induced a transient increase antigen-specific galactosylation sialylation. An initial, afucosylated was membrane-encoding S protein...
The global emergence of antimicrobial resistance is driven by antibiotic misuse and overuse. However, systematic data in Indonesian hospitals to adequately inform policy are scarce.To evaluate patterns quality indicators prescribing six general Jakarta, Indonesia.We conducted a hospital-wide point prevalence survey (PPS) between March August 2019, using Global-PPS WHO-PPS protocols. analysis focused on antibacterials (antibiotics) for systemic use.Of 1602 inpatients, 993 (62.0%) received ≥1...
Community-acquired pneumonia (CAP) can be caused by a variety of pathogens, which Streptococcus pneumoniae, Influenza and currently SARS-CoV-2 are the most common. We sought to identify shared pathogen-specific host response features directly comparing different aetiologies CAP.We measured 72 plasma biomarkers in cohort 265 patients hospitalized for CAP, all sampled within 48 hours admission, 28 age-and sex matched non-infectious controls. stratified into several pathophysiological domains-...
Sepsis is a life-threatening condition arising from an aberrant host response to infection. Recent single-cell RNA sequencing investigations identified immature bone-marrow-derived CD14
Activated platelets have been implicated in the proinflammatory and prothrombotic phenotype of coronavirus disease 2019 (COVID-19). While it is increasingly recognized that lipids important structural signaling roles platelets, lipidomic landscape during infection has remained unexplored. To investigate platelet lipidome patients hospitalized for COVID-19. We performed untargeted lipidomics 25 COVID-19 23 noninfectious controls with similar age sex characteristics, comparable comorbidities....
Introduction Immune response dysregulation has been implicated in the development of intensive care unit (ICU)-acquired pneumonia. We aimed to determine differences longitudinal blood transcriptional between patients who develop ICU-acquired pneumonia (cases) and those do not (controls). Methods performed a case-cohort study mechanically ventilated trauma surgery with ICU stays >2 days, enrolled 30 hospitals across Europe. collected for RNA sequencing at baseline, day 7 (in cases)...
Introduction Platelets play a crucial role in the host response to infection, amongst other through release of proinflammatory and procoagulant proteins. Pneumonia represents major disease burden, which has further increased since onset corona virus (COVID)-19 pandemic. We aimed determine platelets’ protein profiles hospitalised patients with non-COVID community-acquired pneumonia (CAP) or COVID-19. Methods from CAP COVID-19, control subjects were incubated platelet receptor agonists...
Abstract Lessons Learned Motivating patients to enroll in chemopreventive studies is challenging. Chemoprevention with toxic drugs not feasible. Background LKB1 mutations are the underlying genetic abnormality causing Peutz-Jeghers syndrome (PJS) and a potential target for everolimus. In this phase II study, efficacy of everolimus on polyp tumor growth PJS was investigated. Methods Adult proven mutation who were suitable treatment included two different cohorts: (a) unresectable malignancies...
Detecting SARS-CoV-2 antibodies may help to diagnose COVID-19. Head-to-head validation of different types immunoassays in well-characterized cohorts hospitalized patients remains needed.We validated three chemiluminescence (CLIAs) (Liaison, Elecsys, and Abbott) one single molecule array assay (SIMOA) (Quanterix) for automated analyzers, rapid immunoassay RIA (AllTest), ELISA (Wantai) parallel first samples from 126 PCR confirmed COVID-19 158 pre-COVID-19 patients. Specificity the AllTest was...
To optimise the use of available SARS-CoV-2 vaccines, some advocate delaying second vaccination for individuals infected within six months. We studied whether post-vaccination immune response is equally potent in over months prior to vaccination.