A5077620186- Milk Quality and Mastitis in Dairy Cows
- Animal Disease Management and Epidemiology
- Tissue Engineering and Regenerative Medicine
- Clostridium difficile and Clostridium perfringens research
- Angiogenesis and VEGF in Cancer
- Botulinum Toxin and Related Neurological Disorders
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Congenital heart defects research
- Animal health and immunology
- Pharmaceutical studies and practices
- Legal Issues in South Africa
- Vector-Borne Animal Diseases
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- T-cell and Retrovirus Studies
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- Environmental Conservation and Management
- Cardiovascular Function and Risk Factors
- Single-cell and spatial transcriptomics
- Veterinary Practice and Education Studies
- Electrospun Nanofibers in Biomedical Applications
- Linguistics and language evolution
- Transgenic Plants and Applications
University Medical Center Utrecht
2016-2024
Utrecht University
2015-2024
Erasmus MC
2016-2017
Netherlands Heart Institute
2017
Ministry of Industry and Trade
2016
Heidelberg University
2016
University Hospital Heidelberg
2016
Amsterdam UMC Location Vrije Universiteit Amsterdam
2016
Universidade do Porto
2016
Pfizer (United States)
2012
Background— The combination of cardiac and renal disease driven by metabolic risk factors, referred to as cardiorenal syndrome (CRMS), is increasingly recognized a critical pathological entity. contribution (micro)vascular injury CRMS considered be substantial. However, mechanistic studies are hampered lack in vivo models that mimic the natural onset disease. Here, we evaluated coronary microvasculature during development obese diabetic Zucker fatty/Spontaneously hypertensive heart failure...
Vascular endothelial (VE) cadherin is a key component of adherens junctions (AJs) and plays an important role in maintaining vascular integrity. Endocytosis VE-cadherin regulates junctional strength decrease surface reduces stability. However, disruption AJs also requirement for sprouting. Identifying novel regulators endocytosis could enhance our understanding angiogenesis. Here, we evaluated the angiogenic potential (CKLF-like MARVEL transmembrane domain 4) CMTM4 assessed which molecular...
We present a microfluidic vascular device. Vascular cells in 3D-ECM environment support hemodynamic flow and enable monocyte interaction.
BACKGROUND: Realistic reconstruction of the in vivo human atherosclerotic environment requires coculture different cell types arranged vessel–like structures with exposure to flow and circulating cells, presenting challenges for disease modeling. This study aimed develop a 3-dimensional tubular microfluidic model quadruple aortic smooth muscle umbilical cord vein endothelial foam cells recreate complex vessel vitro effects immune cells. METHODS: We developed protocol utilizing BFP (blue...
Decrease in VE-cadherin adherens junctions reduces vascular stability, whereas disruption of is a requirement for neovessel sprouting during angiogenesis. Endocytosis plays key role regulating junctional strength by altering bioavailability cell surface proteins, including VE-cadherin. Identification new mediators endothelial endocytosis could enhance our understanding Here, we assessed the function CMTM3 (CKLF-like MARVEL transmembrane domain 3), which have previously identified as highly...
Abstract Microvascular homeostasis is strictly regulated, requiring close interaction between endothelial cells and pericytes. Here, we aimed to improve our understanding of how microvascular crosstalk affects Human-derived pericytes, cultured in absence, or presence human cells, were studied by RNA sequencing. Compared with mono-cultured a total 6704 genes differentially expressed co-cultured Direct contact induced transcriptome profiles associated pericyte maturation, suppression...
H3K27ac histone acetylome changes contribute to the phenotypic response in heart diseases, particularly end-stage failure. However, such epigenetic alterations have not been systematically investigated remodeled non-failing human hearts. Therefore, valuable insight into cardiac dysfunction early remodeling is lacking. This study aimed reveal acetylation of chromatin regions myocardial and their correlations transcriptional neighboring genes. We detected with differential activity (DARs;...
The formation of cell-cell and cell-extra cellular matrix (ECM) contacts by endothelial cells (ECs) is crucial for the stability integrity a vascular network. We previously identified cingulin-like 1 (Cgnl1) in transcriptomic screen new angiogenic modulators. Here we aim to study function junction associated protein Cgnl1 during vessel formation.Unlike family member cingulin, expression enriched ECs growth. important multicellular tubule structures, as shown vitro using loss-of assays 3D...
Impairment of the endothelial barrier leads to microvascular breakdown in cardiovascular disease and is involved intraplaque haemorrhaging progression advanced atherosclerotic lesions that are vulnerable rupture. The exact mechanism regulates vascular integrity requires further definition. Using a microarray screen for angiogenesis-associated genes during murine embryogenesis, we identified thrombospondin type I domain 1 (THSD1) as new putative angiopotent factor with unknown biological...
Abstract 3D‐scaffold based in vitro human tissue models accelerate disease studies and screening of pharmaceutics while improving the clinical translation findings. Here is reported use induced pluripotent stem cell (hiPSC)‐derived vascular organoid cells as a new source for creation an electrospun polycaprolactone‐bisurea (PCL‐BU) 3D‐scaffold‐based, perfused macrovessel model. A separation protocol developed to obtain monocultures organoid‐derived endothelial (ODECs) mural (ODMCs) from...
Formation of a functional vascular system is essential and its formation highly regulated process initiated during embryogenesis, which continues to play important roles throughout life in both health disease. In previous studies, Fzd5 was shown be critically involved this here we investigated the molecular mechanism by endothelial loss receptor attenuates angiogenesis. Using short interference RNA-mediated loss-of-function assays, function signaling via studied human cells (ECs). Our...
Cell-based approaches using tissue engineering and regenerative medicine to replace damaged renal with 3D constructs is a promising emerging therapy for kidney disease. Besides living cells, template provided by scaffold based on biomaterials bioactive factors needed successful engineering. Nature's own scaffolding system the extracellular matrix (ECM). Research has focused mapping mature ECM; however, developing fetal ECM matches more active environment required in constructs. Here, we...
Angiogenesis is crucial for normal development and homeostasis, but also plays a role in many diseases including cardiovascular diseases, autoimmune cancer. Granzymes are serine proteases stored the granules of cytotoxic cells, have predominantly been studied their pro-apoptotic upon delivery target cells. A growing body evidence emerging that granzymes display extracellular functions, which largely remain unknown. In present study, we show granzyme K (GrK) inhibits angiogenesis triggers...
Vascular smooth muscle cells (VSMCs) play a pivotal role in vascular homeostasis, with dysregulation leading to complications. Human-induced pluripotent stem-cell (hiPSC)-derived VSMCs offer prospects for personalized disease modeling and regenerative strategies. Current research lacks comparative studies on the impact of three-dimensional (3D) substrate properties under cyclic strain phenotypic adaptation hiPSC-derived VSMCs. Here, we aim investigate intrinsic properties, such as hydrogel's...
In vascular tissue engineering strategies, the addition of vascular-specific extracellular matrix (ECM) components may better mimic in vivo microenvironment and potentially enhance cell–matrix interactions subsequent growth. For this purpose, exact composition human ECM first needs to be fully characterized. Most research has focused on characterizing mature tissue; however, developing fetal matches active environment required more closely. Consequently, we characterized protein (fetal)...
Abstract Background Atherosclerosis is a complex inflammatory vascular disease characterized by lipid and immune cells accumulation in the vessel wall, leading to lumen narrowing. Although several 3D vitro microfluidic systems were previously described, realistic reconstruction of vivo human atherosclerotic environment requires co-culture different cell types arranged vessel-like structures with exposure flow circulating cells, creating challenges for modelling. In this study we developed...
The urogenital tract is a target for many congenital and acquired diseases, both benign oncogenic. In males, the urethra that transports urine semen can be obstructed by fibrotic disease called urethral stricture (USD). severe USD, whole organ including vascular embedding, corpus spongiosum (CS), affected. Recurrent or USD treated reconstructive surgery. Tissue engineering may improve outcome of reconstruction in patients with complicated USD. Currently only epithelial layer replaced, no...
The endothelial linings of capillaries, such as those in the kidney and small intestines, possess fenestrae that facilitate fluid exchange molecules. Alterations size number have been implicated pathogenesis various diseases. re-creation fenestrated endothelium using human induced pluripotent stem cells (hiPSCs) provides a promising avenue to investigate involvement disease mechanisms pharmacodynamics. In this project, we aim induce formation nonfenestrated hiPSCs-derived (hiPSC-ECs)....
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Dutch CardioVascular Alliance (an initiative with support the Heart Foundation) Grant 2020B008 RECONNEXT, and German Center for Cardiovascular Research (DZHK; 81Z0600207) Background failure preserved ejection fraction lacks targeted therapies, due to insufficient understanding pathogenesis. A multiple comorbidity swine model (MCS) was developed by exposure three risk factors six...