A5078314420- Endometriosis Research and Treatment
- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Uterine Myomas and Treatments
- Immune Cell Function and Interaction
- Estrogen and related hormone effects
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Regulation of Appetite and Obesity
- Galectins and Cancer Biology
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Psoriasis: Treatment and Pathogenesis
- Pregnancy-related medical research
- Cancer-related Molecular Pathways
- Contact Dermatitis and Allergies
- Cancer Genomics and Diagnostics
- Pelvic floor disorders treatments
Medical University of Lublin
2015-2024
Lublin Oncology Center
2006
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2003
Instytut Medycyny Wsi im. Witolda Chodźki
2000-2001
Myeloid-derived suppressor cells (MDSCs) expansion is a hallmark of cancer. Three major MDSC subsets defined as monocytic (M)-MDSCs, polymorphonuclear (PMN)-MDSCs and early stage (e)MDSCs can be revealed in human diseases. However, the clinical relevance immunosupressive pattern these epithelial ovarian cancer (EOC) are unknown. Therefore, we performed comprehensive analysis each subset factors peripheral blood (PB), peritoneal fluid (PF) tumor tissue (TT) samples from EOC integrated this...
Unfortunately, ovarian cancer is still diagnosed most often only in an advanced stage and also the lethal gynecological cancer. Another problem fact that treated patients have a high risk of disease recurrence. Moreover, very diverse terms molecular, histological features mutations. Many may develop platinum resistance, resulting poor response to subsequent lines treatment. To improve prognosis with cancer, it expected make better existing implement new, promising treatment methods. Targeted...
Context: A newly discovered hormone resistin has been shown to be increased in women with polycystic ovary syndrome (PCOS). Objective: The purpose of this study was confirm concentrations PCOS and test the direct effect on human theca cell androgen production. Design: Resistin measured fasting serum samples by RIA. To effects ovarian biosynthesis, cells were cultured for 3 d presence absence forskolin insulin. Patients: Fasting obtained from 45 74 regularly cycling premenopausal control...
Human skin fibroblasts may be the target cells for estrogens. The aim of present study was to confirm presence both isoforms estrogen receptors (ER) in these cells. Experiments were carried out primary cultures human fibroblasts. ER-alpha and ER-beta mRNAs measured by quantitative assays based on reverse transcription (RT) mRNA polymerase chain reaction (PCR) amplification cDNA. To determine which ER their intracellular locations immunohistochemical staining performed. MCF-7 culture a...
Abstract Background Previous studies have shown clinical relevance of programmed death-ligand 1 (PD-L1) and soluble PD-L1 (sPD-L1) in human cancers. However, still contradictory results exist. Our aim was evaluation PD-L1-expressing monocytic myeloid-derived suppressor cells (M-MDSCs), monocytes/macrophages (MO/MA), tumour (TC) immune/inflammatory (IC) as well investigation the sPD-L1 ovarian cancer (OC) patients. Methods The group 74 pretreatment women were enrollment to study. expression...
The latest literature demonstrates the predominant role of programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate clinical importance PD-L1/PD-L2 expression OC environments. We evaluated patients (n = 53). analysis was performed via flow cytometry on myeloid (mDCs) and plasmacytoid dendritic cells (pDCs) monocytes/macrophages (MO/MA) peripheral blood, peritoneal fluid (PF), tumor...
Abstract Epigenetic silencing including histone modifications and DNA methylation is an important tumorigenic mechanism. However, its role in cancer immunopathology immunotherapy poorly understood. Using human ovarian cancers as our model, here we show that enhancer of zeste homologue 2 (EZH2)-mediated H3 lysine 27 trimethylation (H3K27me3) methyltransferase 1 (DNMT1)-mediated repress the tumour production T helper (TH1)-type chemokines CXCL9 CXCL10, subsequently determine effector T-cell...
Immune system dysregulation is clinically evident in the pathogenesis of endometriosis (EMS). Changes dendritic cells (DCs) activity or phenotype may be involved implantation and growth endometrial tissue outside uterus disease. The TIM-3/Gal-9 axis implicated development immune tolerance. However, knowledge about exact role this pathway EMS extremely poor. In present study, we evaluated expression Gal-9 on myeloid DCs (mDCs) plasmacytoid (pDCs) peripheral blood (PB) peritoneal fluid (PF)...
The molecular bases of miR‐182 deregulation in epithelial ovarian cancers (EOCs) remain unknown and its diagnostic or prognostic role EOCs is still unclear. We performed expression analysis using a microarray approach real‐time PCR (qPCR). also used array comparative genomic hybridization methylated DNA immunoprecipitation to study copy number changes methylation aberrations within coding locus/promoter sequences EOC tissues, respectively. have found that significantly increased ( P <...
Ovarian cancer (OC) is one of the most lethal gynecological malignancies. Recent studies suggest a crucial role PD-1/PD-L1 pathway in OC pathogenesis. Therefore, our study aimed at evaluation clinical importance PD-1 expression ovarian patients.In this study, we investigated patients (n=50) by analyzing its on CD4+ and CD8+ T cells three environments: peripheral blood (PB), peritoneal fluid (PF), tumor (TT) as well soluble (sPD-1) plasma PF terms their prognostic significance. with were...
The interaction between dendritic cells (DCs) and T mediated by the programmed cell death 1 (PD-1)/programmed ligand (PD-L1)/programmed 2 (PD-L2) pathway is most important point in regulating immunological tolerance autoimmunity. Disturbances quantity, maturity, activity of DCs may be involved implantation growth endometrial tissue outside uterus endometriosis (EMS). However, little known about role immune checkpoint pathways EMS. In our study, we examined expression PD-L1/PD-L2 on myeloid...
There were high hopes for the new antiangiogenic medicament, bevacizumab, which could inhibit creation of blood vessels through binding to isoform A vascular endothelial growth factor (VEGF). However, it is not only that are responsible tumor cell spread. During process growth, lymphangiogenesis mediated by other members VEGF family, specifically VEGF-C and VEGF-D, act independent bevacizumab. Therefore, based on mechanism bevacizumab action processes angio- lymphangiogenesis, we formed...
The balance between ER-α and ER-β in fibroblasts may be crucial the physiological response to ligands. Up- or down-regulation of ERs different compounds could mediate reversal certain age-related changes skin connective tissue. time-dependent effects 17-β estradiol, raloxifene tamoxifen on mRNA expression fibroblast cultures were performed. Experiments carried out primary human obtained from postmenopausal women. cells cultured medium containing: 2 µmol/l estradiol (E2), 4 (Tx) (Rx) for 7,...