A5032323222- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- Seaweed-derived Bioactive Compounds
- Ovarian cancer diagnosis and treatment
- Heat shock proteins research
- Single-cell and spatial transcriptomics
- Cytokine Signaling Pathways and Interactions
- Angiogenesis and VEGF in Cancer
- Polysaccharides and Plant Cell Walls
- Endoplasmic Reticulum Stress and Disease
- Enzyme function and inhibition
- Globalization, Economics, and Policies
- Nigella sativa pharmacological applications
- Endometrial and Cervical Cancer Treatments
- Phytochemistry and biological activity of medicinal plants
- Infectious Disease Case Reports and Treatments
- ATP Synthase and ATPases Research
- Chemokine receptors and signaling
University of Michigan–Ann Arbor
2021-2025
Medical University of Lublin
2015-2024
Myeloid-derived suppressor cells (MDSCs) expansion is a hallmark of cancer. Three major MDSC subsets defined as monocytic (M)-MDSCs, polymorphonuclear (PMN)-MDSCs and early stage (e)MDSCs can be revealed in human diseases. However, the clinical relevance immunosupressive pattern these epithelial ovarian cancer (EOC) are unknown. Therefore, we performed comprehensive analysis each subset factors peripheral blood (PB), peritoneal fluid (PF) tumor tissue (TT) samples from EOC integrated this...
Immune tolerance mechanisms are shared in cancer and pregnancy. Through cross-analyzing single-cell RNA-sequencing data from multiple human types the maternal-fetal interface, we found B7-H4 (VTCN1) is an onco-fetal immune checkpoint. We showed that genetic deficiency of resulted activation fetal resorption allogeneic pregnancy models. Analogously, contributed to MPA/DMBA-induced breast progression, accompanied by CD8
Tumor-associated macrophages (TAMs) shape tumor immunity and therapeutic efficacy. However, it is poorly understood whether how post-translational modifications (PTMs) intrinsically affect the phenotype function of TAMs. Here, we reveal that peptidylarginine deiminase 4 (PAD4) exhibits highest expression among common PTM enzymes in TAMs negatively correlates with clinical response to immune checkpoint blockade. Genetic pharmacological inhibition PAD4 prevents progression tumor-bearing mouse...
The anticancer activity of ethanol extract isolated from Goji berry (EEGB) on T47D human breast cancer cell line has been reported. Cell viability and proliferation were examined with the use BrdU, MTT NR methods. Induction apoptosis was assessed by propidium iodide Hoechst 33342 staining. Expression genes involved in proliferation, apoptosis, cycle control regulation transcription estimated using Western blotting analysis. EEGB inhibited cells a time-, dose-dependent manner. study confirmed...
Abstract Background Previous studies have shown clinical relevance of programmed death-ligand 1 (PD-L1) and soluble PD-L1 (sPD-L1) in human cancers. However, still contradictory results exist. Our aim was evaluation PD-L1-expressing monocytic myeloid-derived suppressor cells (M-MDSCs), monocytes/macrophages (MO/MA), tumour (TC) immune/inflammatory (IC) as well investigation the sPD-L1 ovarian cancer (OC) patients. Methods The group 74 pretreatment women were enrollment to study. expression...
Myeloid-derived suppressor cells (MDSCs) play a significant role in the immune system and have been extensively studied cancer. MDSCs are heterogeneous population of myeloid that accumulate tumor microenvironment. Consequently, high abundance these often leads to immunosuppression, growth, treatment failure, poor prognosis. Ovarian cancer ranks fifth deaths among women, accounting for more than any other female genital tract. Currently, there is lack effective clinical strategies ovarian...
Ovarian cancer remains the most lethal gynecologic malignancy. This is due to lack of effective screening, diagnosis predominance in late stage disease, a high recurrence rate after primary therapy, and poor treatment response platinum-resistant tumor. Thus, unique biomarkers, predictive individual disease course, prognosis are urgently needed. The aim our study was assess clinicopathological significance plasma, peritoneal fluid, tumor tissue levels mesothelin epithelial ovarian patients....
The aim of this study was to investigate the influence Notch1 activity level on pharmacological interaction between cisplatin (CDDP) and two histone deacetylase inhibitors (HDIs)-valproic acid (VPA) vorinostat (SAHA) in triple negative breast cancer (TNBC) cells. Stable (BC) cell lines with increased decreased were generated using a transfection method. type CDDP HDIs determined by isobolographic analysis proliferation MDA-MB-231 cells differential levels vitro. combination CDDP/SAHA...
Histone deacetylase inhibitors (HDIs) are a group of compounds that exhibit anticancer activity, but their significance and usefulness in breast cancer (BC) treatment still controversial. The ability cells to invade migrate is augmented by the acquisition mesenchymal phenotype - process known as epithelial-to-mesenchymal transition (EMT). Changes expression level different cadherins, so-called cadherin switches, have been used monitor EMT development tumor progression, particular migration...
Background: Uterine sarcomas and carcinosarcoma are associated with unfavorable prognosis. The regimens that used in chemotherapy high incidence of side effects usually do not significantly increase patients’ survival rates. In this study we investigated the activity interactions between gemcitabine fucoidan, natural compound known for its anti-tumor properties, human cell models. Methods: SK-UT-1, SK-UT1-B (carcinosarcoma), MES-SA (leiomyosarcoma), ESS-1 (endometrial stromal sarcoma) lines...
Introduction: mTOR inhibitors are anticancer agents affecting mTOR/AKT/PI3K pathway that is one of the most important in human cancer cells.Hyperactivation and overexpression this members frequently reported uterine sarcoma carcinosarcoma.Present study aimed to assess activity two (rapamycin -RAP sapanisertib -MLN) as a single agent combined with gemcitabine (GEM, substances commonly used systemic treatment) carcinosarcoma vitro models.Material methods: SK-UT-1 SK-UT1-B (uterine...
Background: Ovarian cancer (OC) is the most lethal malignancy of female reproductive tract. Consequently, a better understanding malignant features in OC pertinent. Mortalin (mtHsp70/GRP75/PBP74/HSPA9/HSPA9B) promotes development, progression, metastasis, and recurrence. Yet, there no parallel evaluation clinical relevance mortalin peripheral local tumor ecosystem patients. Methods: A cohort 92 pretreatment women was recruited, including 50 patients, 14 patients with benign ovarian tumors,...
The aim of this study was to evaluate the influence ovarian cancer cell lysates isolated from type I or II (OC) on phenotype monocyte-derived dendritic cells (Mo-DCs) and cytokine profile. We also determined whether Mo-DCs tumor microenvironment, reflected by peritoneal fluid (PF) cancer, could promote regulatory T (Tregs) differentiation naive CD4+ lymphocytes in vitro.Our results show a significant role microenvironment PF OC inhibition DC process. Interestingly, percentage co-expressing...
The role of angiogenesis in leiomyosarcomas still remains unclear. aim this study was to evaluate the NRP1 expression leiomyosarcoma tissues and find relations between its clinical features.The group consisted 50 patients with diagnosis uterine leiomyosarcoma. Clinical follow up data were collected. Using immunohistochemical methods detected.The lack found 14 cases, positive (weak or moderate) noted 36 cases. significantly higher observed more severe stages comparison lower disease. shorter...