Abstract Maternally expressed gene 3 (MEG3), a long non-coding RNA (lncRNA), has tumor-suppressor properties and its expression is lost in several human tumors. However, biological role esophageal squamous cell carcinoma (ESCC) tumorigenesis poorly defined. The present study determined the methylation status of MEG3 cancer cells ESCC clinical specimens, further observed competing endogenous (ceRNA) activity pathogenesis development ESCC. Significant downregulation was detected tissues level...

As an important long noncoding RNA, Hox transcript antisense intergenic RNA (HOTAIR) is involved in the development and progression of various carcinomas. However, role genetic alterations HOTAIR gastric cardia adenocarcinoma (GCA) occurrence have not been elucidated. We performed a case-control study population north China to evaluate possible association between haplotype-tagging SNPs (htSNPs) whole sequence risk GCA as well functional effect susceptibility single nucleotide polymorphism...

Marein, an active component of the Coreopsis tinctoria Nutt. plant, is known to improve diabetic nephropathy (DN). However, its anti-diabetic functions in DN and potential mechanisms remain unclear. The aim this study was elucidate effects Marein db/db mice with DN, high glucose–treated HK-2 cells. In vivo, treating for 12 consecutive weeks restored diabetes-induced hyperglycemia dyslipidemia, ameliorated renal function deterioration, glomerulosclerosis, ectopic lipid deposition. exerted...

Recent studies provide strong evidence that the androgen receptor (AR) signaling pathway remains active in castration-resistant prostate cancer (CRPC). However, underlying mechanisms are not well understood. In this study, we demonstrate plant homeo domain finger protein 8 (PHF8 )interacts with and functions as an essential histone demethylase activity-dependent AR coactivator. Furthermore, expression of PHF8 is induced by hypoxia various cell lines. Knockdown either hypoxia-inducible factor...

Abstract Natural antisense lncRNAs can interfere with their corresponding sense transcript to elicit concordant or discordant regulation. LncRNA ZNF667-AS1 and its gene ZNF667 were found be downregulated in esophageal squamous cell carcinoma (ESCC) tissues by RNA sequencing; however, the exact roles of both genes ESCC occurrence development have not been clarified. This study was investigate expression patterns, epigenetic inactivation mechanisms, function, prognostic significance...

Maternally expressed gene 3 (MEG3) is an imprinted located at 14q32 which encodes lncRNA and downregulated in expanding list of cancer cell lines primary human cancers. The miR-770 transcribed from the intronic sequence MEG3 may be host for miR-770. However, biological role gastric cardia adenocarcinoma (GCA) development prognosis poorly defined. present study was to investigate function methylation status GCA, further detect functional association its GCA carcinogenesis prognosis....

Carboxymethylated pachyman (CMP) is characterized by immune regulatory, antitumor and antioxidant activities. However, whether CMP contributes to the treatment of ovarian cancer has yet be explored. The role in cell death was analyzed using CCK-8 flow cytometry assays. data showed that induced a dose-dependent manner. Furthermore, CMP-induced could largely reversed preincubation with ferrostatin-1 (Fer-1) but not 3-methyladenine or necrostatin-1. Reverse transcription-quantitative PCR...

Abstract Brassica napus , an allotetraploid used as oilseed crop, vegetable, or feed possesses significant economic and medicinal value. Although the CHYR gene family has been functionally characterised in various aspects of plant growth, development, stress responses, its systematic investigation B. is lacking. In contrast to seven genes ( AtCHYR1-AtCHYR7 ) identified Arabidopsis thaliana nine orthologues were detected rapa oleracea while 24 found . This discrepancy consistent with...

Abstract Background The aerial parts of land plants are covered with cuticular waxes that limit non-stomatal water loss and gaseous exchange, protect from ultraviolet radiation pathogen attack. This is the first report on characterization genetic mapping a novel dominant glossy mutant ( BnaA.GL ) in Brassica napus . Results Transmission electron microscopy revealed cuticle ultrastructure GL leaf stem were altered dramatically compared wide type (WT). Scanning corroborated reduction wax...

As seed oil content (OC) is a key measure of rapeseed quality, better understanding the genetic basis OC would greatly facilitate breeding high-oil cultivars. Here, we investigated components effects and genotype × environment interactions (GE) that govern using full diallel set nine parents, which represented wide range Chinese cultivars pure lines with various OCs.Our results from an embryo-cytoplasm-maternal (GoCGm) model for diploid seeds showed was primarily determined by (VG) GE (VGE),...

The growth arrest DNA damage‐inducible gene (GADD45) family, which is composed of GADD45A, GADD45B, and GADD45G, may play similar but not identical roles in tumorigenesis. Genetic changes associated with or responsible for their dysregulation are general uncommon. This study was to detect the role GADD45 family gastric cardia adenocarcinoma (GCA) relationship GADD45A GADD45G methylation a series pathological parameters large GCA sample, order elucidate more information on regard pathogenesis...

BACKGROUND F‐box protein 32 (FBXO32) (also known as atrogin‐1), a member of the family, has recently been identified transforming growth factor beta (TGF‐β)/Smad target gene involved in regulating cell survival, and it may be transcriptionally silenced by epigenetic mechanisms some kinds carcinomas, yet its role esophageal squamous carcinoma (ESCC) not defined. METHODS The FBXO32 ESCC correlation methylation with series pathologic parameters were studied large cohort patients ESCC. RESULTS...

MiRNAs regulate gene expression and play pivotal roles in biological processes. can be inactivated by epigenetic mechanisms, such as DNA hypermethylation of CpG sites within islands. Here, we investigated the role methylation status miR-203a miR-203b esophageal cancer cell lines primary squamous carcinoma (ESCC) tumors further elucidate both miRNAs prognosis ESCC. The present study revealed a strong downregulation ESCC samples. Treatment cells with demethylating agent 5-Aza-dC led to...

The effects of different substrate stiffness were investigated on epithelial-mesenchymal transition (EMT) cervical cancer cell lines and the role miR-106b its target protein DAB2 therein. Cervical HeLa SiHa cultured artificial substrates with prepared using ratios acrylamide bis-acrylamide. Changes microRNA profiles detected chip analysis, expression levels EMT-related markers E-cadherin vimentin western blotting real-time PCR. In addition, overexpression as well knockdown also examined...

Ras-association domain family 1A (RASSF1A) gene, a candidate tumor suppressor is inactivated in several human tumors, usually by hypermethylation of its promoter region. RASSF1A induces cell cycle arrest through inhibition cyclin D1 accumulation. In this work, the methylation status 92 gastric cardia adenocarcinoma (GCA) and corresponding normal tissues were investigated using Methylation-specific PCR (MSP) approach, immunohistochemistry method RT-PCR used respectively to examine protein...

The RAS-association domain family (RASSF) consists of 10 members, and several members act as tumor suppressor genes epigenetically inactivated in different types. present study investigated the role methylation status RASSF2, RASSF3, RASSF4, RASSF6 pathogenesis prognosis GCA. Quantitative real-time RT-PCR, Western blot, immunohistochemistry (IHC) methods were used respectively to detect expression 135 GCA cases BS-MSP method was clarify these four genes. Decreased mRNA protein detected...

As a long non‐coding RNA, C5orf66‐AS1 is located at 5q31.1. Downregulation and aberrant hypermethylation of have been detected in limited several tumors. However, the biological role distribution methylated CpG sites gastric cardia adenocarcinoma (GCA) development prognosis are poorly clarified. The present study was to investigate expression status function GCA, detect within three islands promoter gene body C5orf66‐AS1, further clarify its prognostic value GCA patients. significantly...

Countless evidence suggests that long noncoding RNAs (lncRNAs) are involved in human malignant cancers, including esophageal squamous cell carcinoma (ESCC), although their exact function remains unclear. In the present study, we aimed to investigate roles and molecular mechanisms of lncRNA LOC440173 ESCC progression. Microarray analysis quantitative real-time polymerase chain reaction were conducted measure expression levels miR-30d-5p. The biological this was investigated using...

The canonical Wnt signalling pathway plays a key role during embryogenesis and pathogenesis of various types tumors.Recently, several studies have shown that the promoter hypermethylation Wnt-antagonist genes, including sFRP-1, sFRP-2, sFRP-4, sFRP-5, Wif-1 Dkk-3, been certified to contribute tumorigenesis cancers.The aim this study was investigate methylation genes in gastric cardia adenocarcinoma (GCA) corresponding adjacent non-cancerous tissues, establish possible relationship between...

BACKGROUND Although downregulation of caveolin-1 (Cav-1), which is a key constituent membrane caveolae and regulator cellular processes, associated with colorectal cancer (CRC), its involvement in the disease progression largely unknown. This study aimed to explore role Cav-1 CRC mechanisms. MATERIAL AND METHODS Fresh tissues from patients human SW480 cells were used evaluate Ki-67 expression using immunohistochemistry Western blotting. The MTS Transwell assays performed determine effects...