A5101594784- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- Neonatal Respiratory Health Research
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Occupational and environmental lung diseases
- Pulmonary Hypertension Research and Treatments
- Sarcoidosis and Beryllium Toxicity Research
- Lung Cancer Treatments and Mutations
- IL-33, ST2, and ILC Pathways
- Systemic Sclerosis and Related Diseases
- Sphingolipid Metabolism and Signaling
- Tracheal and airway disorders
- Mycobacterium research and diagnosis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Advanced MRI Techniques and Applications
- Antifungal resistance and susceptibility
- Immune cells in cancer
- Inflammatory Myopathies and Dermatomyositis
- Pleural and Pulmonary Diseases
- Fungal Plant Pathogen Control
- Vascular Anomalies and Treatments
- Colorectal Cancer Surgical Treatments
- Synthesis and biological activity
Tokushima University
2017-2024
Juntendo University
2024
Toho University
2014-2021
Juntendo University Hospital
2021
Center for Rheumatology
2020-2021
Nagasaki University
2021
Kindai University
2021
Okayama University
2021
Osaka University of Pharmaceutical Sciences
2021
Toho University Omori Medical Center
2017
Nintedanib, a tyrosine kinase inhibitor that is specific for platelet-derived growth factor receptors (PDGFR), fibroblast (FGFR), and vascular endothelial (VEGFR), has recently been approved idiopathic pulmonary fibrosis. Fibrocytes are bone marrow-derived progenitor cells produce factors contribute to fibrogenesis in the lungs. However, effects of nintedanib on functions fibrocytes remain unclear. Human monocytes were isolated from peripheral blood healthy volunteers. The expression their...
Platelet-derived growth factor (PDGF) has been implicated in the pathogenesis of pulmonary fibrosis. Nintedanib, a multi-kinase inhibitor that targets several tyrosine kinases, including PDGF receptor (PDGFR), was recently approved as an anti-fibrotic agent to reduce deterioration FVC patients with idiopathic fibrosis (IPF). However, effects PDGFR-α or -β on remain unclear. In attempt clarify their effects, we herein used blocking antibodies specific for (APA5) and (APB5) bleomycin...
Recent clinical trials revealed that immune checkpoint inhibitors and antiangiogenic reagent combination therapy improved the prognosis of various cancers. We investigated roles fibrocytes, collagen-producing monocyte-derived cells, in immunotherapy. Anti-VEGF (vascular endothelial growth factor) antibody increases tumor-infiltrating fibrocytes enhances antitumor effects anti-PD-L1 (programmed death ligand 1) vivo. Single-cell RNA sequencing CD45+ cells identifies a distinct "fibrocyte...
ABSTRACT We evaluated the usefulness of an Aspergillus galactomannan (GM) test, a β- d -glucan (βDG) and two different PCR assays bronchoalveolar lavage fluid (BALF) samples for diagnosis chronic pulmonary aspergillosis (CPA). BALF from 30 patients with 120 without CPA were collected. calculated sensitivity, specificity, positive predictive value, negative likelihood ratio, diagnostic odds ratio each test individually in combination other tests. The optical density index values, as...
Purpose/Aim of the Study: Wnt/β-catenin signaling was reported to be activated in pulmonary fibrosis, and focused on as a target for antifibrotic therapy. However, mechanism how inhibition ameliorate fibrosis has not been fully elucidated. The purpose this study is explore cells examine effect novel inhibitor PRI-724 specifically disrupting interaction β-catenin CBP. Materials Methods: C-82, an active metabolite PRI-724, expression TGF-β1 α-smooth muscle actin (SMA) examined fibroblasts...
Various factors affecting the demulsification rate of emulsion were examined by means an electrostatic coalescence method using which consisted organic phase containing tri-n-octhylamine (TNOA) and Span 80 or ECA 4360J internal aqueous Na2CO3. Increases in applied voltage, Na2CO3 concentration, WI/O ratio result increase rate, while increases surfactant (including 4360J) agitation speed homogenizer homogenizing time cause a decrease rate. Changes TNOA concentration do not have significant...
Fibrocytes, a distinct population of collagen-producing, monocyte-derived cells, are involved in wound healing as well fibrotic diseases. Recently, fibrocytes have been revealed to play role the tumor microenvironment, particularly under antiangiogenic therapy. In addition, combination cancer immunotherapy with immune checkpoint inhibitor and agents developed for various cancers clinical setting, although immunological background is not clear. current study, we aimed determine function...
The signaling pathways of growth factors, including platelet-derived factor, can be considered specific targets for overcoming the poor prognosis idiopathic pulmonary fibrosis. Nintedanib, recently approved multiple kinase inhibitor, has shown promising antifibrotic effects in patients with fibrosis; however, its efficacy is still limited, and some cases, treatment discontinuation necessary owing to toxicities such as gastrointestinal disorders. Therefore, more effective agents less toxicity...
Abstract Background Idiopathic pulmonary fibrosis (IPF) is a fatal fibrotic lung disease with few effective therapeutic options. Recently, drug repositioning, which involves identifying novel potentials for existing drugs, has been popularized as new approach the development of reagents. However, this not yet fully utilized in field fibrosis. Methods The present study identified options using systematic computational repositioning based on integration public gene expression signatures and...
Background: Lysophosphatidic acid (LPA), a prototypic member of large family lysophospholipids, has been recently shown to play role in immune responses respiratory diseases. The involvement LPA allergic airway inflammation reported, but the mechanism remains unclear. Object: We analyzed biological activity vitro and vivo investigated its mice using an receptor 2 (LPA2) antagonist. Methods: used murine model with acute inflammation, which are sensitized challenged house dust mite,...
Anti-aminoacyl-tRNA synthetase (ARS) antibodies form a condition called Antisynthetase syndrome (ASSD). While interstitial lung disease (ILD) is particularly frequent manifestation of ASSD and closely associated with morbidity mortality, few studies have been conducted on its characteristics high-resolution computed tomography (HRCT). In this study, we clarified the HRCT findings in patients anti-ARS antibody-positive ILD (ARS-ILD). Methods: The at time diagnosis 24 ARS-ILD were...
Objective An acute exacerbation (AE) of idiopathic pulmonary disease (IPF) represents a life threatening condition. The activities daily living (ADL) and quality patients who survive an AE IPF (AE-IPF) are often diminished. However, the association between AE-IPF ADL has yet to be evaluated.
Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis.However, their function fibrosis is unclear.We analyzed compared with that monocytes and localization fibrotic tissues patients idiopathic (IPF).We gene expression profile monocyte-derived fibrocytes by microarray analysis.Proliferation differentiation into myofibroblasts examined 3 H-thymidine incorporation assay Western blotting.We measured level growth...
Abstract TOLLIP polymorphism has been implicated in the development and prognosis of idiopathic pulmonary fibrosis (IPF), mainly whites. However, ethnic differences characteristics other interstitial pneumonia (non-IPF) subtypes are unclear. We evaluated association between rs3750920 genotype clinical Japanese patients with fibrosing lung diseases (ILD). genotyped 102 ILD (75 IPF 27 non-IPF patients) analyzed interaction distribution their characteristics. The overall frequencies C/C, C/T,...
Immune checkpoint inhibitor (ICI) programmed death (PD)-1/PD-ligand 1 (PD-L1) blockade has been approved for various cancers. However, the underlying antitumor mechanisms mediated by ICIs and predictive biomarkers remain unclear. We report effects of anti-PD-L1/PD-1 Ab in tumor angiogenesis. In syngeneic mouse models, anti-PD-L1 inhibited angiogenesis induces net-like hypoxia only ICI-sensitive cell lines. tissue serum line-bearing mice, interferon-γ (IFN-γ) inducible angiostatic chemokines...
Epithelial-mesenchymal transition (EMT) is reported to promote airway remodeling in asthmatics, which the main histological change that causes complex and severe symptoms asthmatics. However, little known about whether EMT also plays a role acute exacerbations of asthma evoked by respiratory tract infections.A human lung adenocarcinoma line, A549, was incubated with TGF-β1 at 10 ng/ml induce EMT. Then cells were stimulated CpG ODN. Expression surface intracellular molecules analyzed flow...
FGFs (fibroblast growth factors) are major factors associated with the pathogenesis of pulmonary fibrosis. On one hand, nintedanib, a tyrosine kinase inhibitor targeting several factor receptors, including FGF receptor (FGFR), has been approved for treatment idiopathic other recent reports suggest that required epithelial recovery. In this study, we focused on signaling to both fibroblasts and alveolar cells (AECs), examined effect pan-FGFR blocker experimental fibrosis in mice. The effects...
Background Lymphocyte-specific protein tyrosine kinase (Lck) is a member of the Src family kinases. The significance Lck inhibition in lung fibrosis has not yet been fully elucidated, even though commonly preceded by inflammation caused infiltration T-cells expressing Lck. In this study, we examined effect an experimental mouse model fibrosis. We also evaluated on expression TGF-β1, inhibitory cytokine regulating immune function, regulatory (Tregs). Methods Lung was induced mice...
Propionibacterium acnes is implicated in the pathogenesis of sarcoidosis. We investigated usefulness immunohistochemistry (IHC) with a commercially available P. acnes-specific monoclonal antibody (PAB antibody) for differentiating sarcoidosis from other granulomatous diseases. Formalin-fixed paraffin-embedded tissue samples 94 patients and 30 control diseases were examined by original manual IHC method. also compared detection frequency sarcoid granulomas between automated methods. was...