Biogenic nanoparticles are the smartest weapons to deal with multidrug-resistant "superbugs" because of their broad-spectrum antibacterial propensity as well excellent biocompatibility. The aqueous biogenic silver (Aq-bAgNPs) and ethanolic (Et-bAgNPs) were synthesized using extracts Andrographis paniculata stem, respectively, reducing agents. Electron microscopic images confirmed synthesis almost spherical shaped (bAgNPs). zeta potentials negative -22 -26 mV for Aq-bAgNPs Et-bAgNPs,...

Thermal engineering at the microscale, such as regulation and precise evaluation of temperature within cellular environments, is a major challenge for basic biological research biomaterials development. We engineered polymeric nanoparticle having fluorescent sensory dye photothermal embedded in polymer matrix, named nanoheater-thermometer (nanoHT). When nanoHT illuminated with near-infrared laser 808 nm, subcellular-sized heat spot generated live cell. Fluorescence thermometry allows...

Gold nanoparticles are inert for the human body, and therefore, they have been functionalized to provide them with antibacterial properties. Here, elongated tetrahexahedral (ETHH) Au were synthesized, characterized, lipoic acid (LA), a natural antioxidant terminal carboxylic dithiolane ring, generate ETHH-LA nanoparticles. The activity of was investigated in vitro, showing that LA enhances 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging Fe3+ ion reducing at higher amounts....

Nanotherapeutics has emerged as the most sought after approach to tackle menace of drug-resistant pathogenic bacteria. Among others, biogenic silver nanoparticles (bAgNPs) synthesized using medicinal plant extracts demonstrate promising antibacterial propensity with excellent biocompatibility. Herein, bAgNPs were through green chemistry Syzygium cymosum leaf extract a reducing agent at different pH values (i.e., 5, 7, 8, and 10). The average size 10 was 23.3, 21.3, 17.2, 35.3 nm,...

An amino acid-based cationic lipid having a TFA counterion (trifluoroacetic acid counterion) in the lysine headgroup was used to deliver functional proteins into human cervical cancer cells, HeLa, presence of serum. Proteins study were fluorescein isothiocyanate (FITC) labeled bovine serum albumin, mouse anti-F actin antibody [NH3], and goat anti IgG conjugated with FITC. The formation liposome/protein complexes confirmed using native polyacrylamide gel electrophoresis. Furthermore,...

Gold (Au) is an inert metal in a bulk state; however, it can be used for the preparation of Au nanoparticles (i.e., AuNPs) multidimensional applications field nanomedicine and nanobiotechnology. Herein, monodisperse concave cube AuNPs (CCAuNPs) were synthesized functionalized with natural antioxidant lipoic acid (LA) tripeptide glutathione (GSH) because different crystal facets provide binding sites distinct ligands. There was ∼10 nm bathochromic shift UV–vis spectrum when CCAuNPs LA, size...

Andrographis paniculata (Kalmegh) has been considered as a medicinal shrub and used plant in the remote areas of Bangladesh. A. leaf stem extracts were prepared using polar (i.e., water, 70% ethanol) nonpolar hexane) solvents. The phytochemical contents, total phenol contents (TPC), antioxidant activity, antibacterial activity all investigated. Both gram-positive Bacillus subtillis) gram-negative E. coli, Salmonella typhi) strains bacteria for assay sample extracts. ethanolic contained...

Among metallic nanoparticles, silver nanoparticles (AgNPs) have a wide spectrum of medical applications. Herein, biogenic (bAgNPs) were prepared from extracts Caesalpinia digyna leaf as reducing agent at different pH values (i.e., 5, 7, 8, and 10). The as-synthesized bAgNPs characterized using UV-vis Fourier transform infrared (FTIR) spectroscopies, scanning transmission electron microscopy, powder X-ray diffraction analysis, dynamic light scattering, ζ-potential analysis. sizes 10 45.4,...

Abstract Polypropylene (PP) mesh is most commonly used for the treatment of hernia and pelvic floor construction. However, some patients have a few complications after surgery due to rejection or infection implanted meshes. The poor biocompatibility PP mesh, low wettability results in cell attachment/proliferation restricts loading antibacterial agent, leading slow healing process high risk surgery. Here this study, new technique has been employed develop novel antimicrobial biocompatible...

Gold nanoparticles (AuNPs) are one of the most extensively studied nanomaterials and their distinct physicochemical properties make them suitable for versatile applications. Herein, we synthesized concave cube-shaped gold (CCAuNPs) functionalized with lactoferrin (Lf), a natural antimicrobial protein, through electrostatic interaction as well weak covalent formation. The functionalization CCAuNPs was confirmed UV–Visible (i.e., bathochromic shift surface plasmon resonance peak by 7 nm),...

The application of nanotherapeutics is being considered as one the most sought-after strategies to combat threat posed by drug resistant bacteria.

Background: Currently available gene delivery vehicles have many limitations such as low efficiency and high cytotoxicity. To overcome these drawbacks, we designed synthesized two cationic lipids comprised of n-tetradecyl alcohol the hydrophobic moiety, 3-hydrocarbon chain spacer, different counterions (eg, hydrogen chloride [HCl] salt or trifluoroacetic acid [TFA] salt) in arginine head group. Methods: Cationic were hydrated 4-(2-hydroxyethyl)-1-piperazineethanesulfonic (HEPES) buffer to...

Polypropylene (PP) surgical mesh has attracted vast attention due to its chemical inertness and excellent mechanical properties. However, improvement is necessary enhance biocompatibility prevent unwanted tissue adhesion. This study addresses these issues through surface modification of plasma-activated PP with a 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer. Reaction time monomer concentration have been optimized achieve the optimal reduction in protein adsorption. Attenuated total...

The delivery of specific genes into neurons offers a potent approach for treatment diseases as well the study neuronal cell biology. Here we investigated capabilities cationic amino acid based lipid assemblies to act nonviral gene vectors in primary cultured neurons. An arginine-based lipid, Arg-C3-Glu2C14, and lysine-based Lys-C3-Glu2C14, with two different types counterion, chloride ion (Cl-) trifluoroacetic (TFA-), were shown successfully mediate transfection plasmid DNA encoding green...

Abstract not availableJahangirnagar University J. Biol. Sci. 6(1): 51-57, 2017 (June)

A potential issue in current nerve guides is that they do not transmit electrical impulses between the distal and proximal end of an injured nerve, i.e. a synapse. Conductivity desirable property ideal guide being considered for peripheral regeneration. Most conductive polymers reported fabrication tissue engineering scaffolds, such as polypyrrole polyaniline, are non-biodegradable possess weak mechanical properties, thus cannot be fabricated into 3D structures. Herein, we have designed new...

Phyllanthus emblica L. belonging to the family Euphorbiaceae is a common medicinal plant in Bangladesh. In order evaluate phytoconstituents and bioactivity of various parts P. emblica, both wild type (i.e., small) cultivated big) fruits were collected from local market Bangladesh six ethanolic extracts prepared for this current study. A comparative analysis phytochemical compositions bioactivities especially antibacterial, antifungal, antioxidant activity accomplished. The qualitative...

With the background of snowballing threat skin wound to public health and economy, this study was undertaken utilizing xanthan gum (Xnt), citric acid (C), gelatin (Gel), glutaraldehyde (G) HPLC-grade water fabricate a series composite hydrogels i.e. Xnt, Xnt:C, Xnt:Gel(3):G, Xnt:C:Gel(3):G, Xnt:Gel(5):G, Xnt:C:Gel(5):G for investigating their healing efficacy in experimental rat model. Physicochemical characterization revealed that all contained more than 90% water. The displayed swelling...

The hydrophobic ionic liquid (IL) 1-butyl-3-methylimidazolium hexafluorophosphate [Bmim][PF6] forms nanostructures with negatively charged plasmid DNA through electrostatic interactions. formation of DNA/IL was confirmed by measuring the zeta potential as well nanostructures. positive, although is charged. positive due to complexation between and positively [Bmim][PF6]. ability protect against ultrasonic shear stress also investigated using an agarose gel electrophoretic assay showed that...

Biogenic silver nanoparticles demonstrate excellent antibacterial activity against a broad range of bacteria. Herein, aqueous biogenic (Aq@bAgNPs) and ethanolic (Et@bAgNPs) were synthesized using as well extracts Diospyros malabarica fruit, respectively. The as-prepared (bAgNPs) characterized UV-Vis, FTIR energy dispersive X-ray (EDS) spectroscopy, electron microscopy, dynamic light scattering spectroscopy (DLS), zetasizer. zeta potentials Aq@bAgNPs Et@bAgNPs −9.8 ± 2.6, −12.2 1.9 mV, bAgNPs...