A5004613388- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Metal-Organic Frameworks: Synthesis and Applications
- Dendrimers and Hyperbranched Polymers
- CRISPR and Genetic Engineering
- Synthesis and biological activity
- Molecular Biology Techniques and Applications
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Nanoparticle-Based Drug Delivery
- Diamond and Carbon-based Materials Research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cancer therapeutics and mechanisms
- Cancer Research and Treatments
- Surgical Sutures and Adhesives
- MXene and MAX Phase Materials
- HIV Research and Treatment
- Biosensors and Analytical Detection
- Microbial Natural Products and Biosynthesis
- Hernia repair and management
- Synthesis and Reactivity of Heterocycles
- Metal complexes synthesis and properties
- Biochemical and Structural Characterization
- Synthesis and Characterization of Heterocyclic Compounds
- Crystallography and molecular interactions
RMIT University
2020-2025
MIT University
2020-2021
Acharya Nagarjuna University
2014
Hindu College of Pharmacy
2014
The detection and identification of biomolecules are essential in the modern era medical diagnostics. Several approaches have been established, but they significant limitations such as laborious time-consuming sample preparation, analysis, need to use external probes which provide adequate not desired levels accuracy sensitivity. Herein, we explored successfully a non-invasive technique detect identifybiomolecules amino acids proteins by utilizing their intrinsic fluorescence. developed...
Metal–organic-frameworks for gene therapy in prostate cancer – ZIF-C based delivery of RNA interference and CRISPR/Cas9 causes host expression knockdown. Coating with a green tea phytochemical enhances uptake increases cytotoxicity.
Metal–organic frameworks (MOFs) provide diverse applications across a wide range of scientific disciplines, including drug/nucleic acid (NA) delivery. In the subclass MOFs, zeolitic imidazolate framework-8 (ZIF-8) is well regarded due to its exceptional physicochemical properties. Biomolecules can be encapsulated and released under precise conditions within ZIF, making it an important material for materials science biomedical applications. Different solvents synthesis methods influence ZIF's...
Abstract Polypropylene (PP) mesh is most commonly used for the treatment of hernia and pelvic floor construction. However, some patients have a few complications after surgery due to rejection or infection implanted meshes. The poor biocompatibility PP mesh, low wettability results in cell attachment/proliferation restricts loading antibacterial agent, leading slow healing process high risk surgery. Here this study, new technique has been employed develop novel antimicrobial biocompatible...
Metal-organic frameworks (MOFs) are an emerging group of nanomaterials for successful biomedical applications in gene therapy. The most commonly biocompatible MOFs zinc-based ZIFs, zirconium-based UiOs, and iron-based MILs. However, despite increasing applications, a comparative study to underscore the critical factors determining effective delivery by such is lacking. Herein, we evaluate potential UiO-66 MIL-88B ZIF-8 therapeutics delivery; revealing importance ZIF-8. Cytotoxicity assays...
Metal-organic frameworks (MOFs) are currently under progressive development as a tool for non-viral biomolecule delivery. Biomolecules such proteins, lipids, carbohydrates, and nucleic acids can be encapsulated in MOFs therapeutic purposes. The favorable physicochemical properties of make them an attractive choice delivering wide range biomolecules including acids. Herein, green fluorescence protein (GFP)-expressing plasmid DNA (pDNA) is used representative to encapsulate within Zn-based...
The preparation of novel PEGylated PAMAM (poly-amidoamine) dendrimers for delivery anti-HIV drug Efavirenz is reported.About 5.0 G are prepared by ethylene diamine core via Michael addition divergent method. PEGylation done polyethylene glycol 600 using epichlorhydrin as linker. loaded with (EFV) evaluated FTIR, DSC, SEM, release, and stability studies.From the results it proved that this method less time consuming, inexpensive, reproducible. Drug-release studies indicate, PAMAM-EFV have...
Metal–organic frameworks (MOFs) are extensively studied materials for delivering a wide range of therapeutic entities including nucleic acids. Protein corona from serum may change biological identity and properties these biocomposites.
Chikungunya fever caused by the mosquito-transmitted chikungunya virus (CHIKV) is a major public health concern in tropical, sub-tropical and temperate climatic regions. The lack of any licensed vaccine or antiviral agents against CHIKV warrants development effective therapies. Small interfering RNA (siRNA) mediated gene silencing structural non-structural genes serves as potential strategy. therapeutic efficiency siRNA can be improved using an efficient delivery system. Metal-organic...
Prostate cancer (PC) is the second leading cause of male deaths, advanced form which continues to be incurable; and nature disease being such that it highly suitable for gene therapy. However, therapy hampered by lack appropriate delivery agents available. Recently, metal-organic-framework (MOF) biocomposites have seen increasing applications in DNA technologies, including delivery. In this work, a polymorph zeolitic imidazolate framework-8 (ZIF-8) MOF nanoparticles called ZIF-C are used as...
Prostate cancer (PC) is the second leading cause of male deaths, advanced form which continues to be incurable; and nature disease being such that it highly suitable for gene therapy. However, therapy hampered by lack appropriate delivery agents available. Recently, metal-organic-framework (MOF) biocomposites have seen increasing applications in DNA technologies, including delivery. In this work, a polymorph zeolitic imidazolate framework-8 (ZIF-8) MOF nanoparticles called ZIF-C are used as...
Telomerase, a ribonucleoprotein coded by the hTERT gene, plays an important role in cellular immortalization and carcinogenesis. is suitable target for cancer therapeutics as its activity highly upregulated 85-90% of cells but absent normal somatic cells. Here, we gene at DNA level applying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 technology encapsulated recently discovered Metal-Organic Framework (MOF). We show that MOF subtype ‘ZIF-C’ can efficiently load...
Telomerase, a ribonucleoprotein coded by the hTERT gene, plays an important role in cellular immortalization and carcinogenesis. is suitable target for cancer therapeutics as its activity highly upregulated 85-90% of cells but absent normal somatic cells. Here, we gene at DNA level applying Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 technology encapsulated recently discovered Metal-Organic Framework (MOF). We show that MOF subtype ‘ZIF-C’ can efficiently load...