Colorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal progression metastasis formation crucial for the identification of novel epigenetic markers patient prognosis. Genome-wide sequencing paired samples colon (normal adjacent, primary tumour lymph node metastasis) showed global hypomethylation CpG island (CGI) hypermethylation tumours compared to normal. In we observed high non-CGI regions...

Context: Aberrant hypermethylation of promoter region tumor suppressor genes could be used as cancer biomarkers.Objective: To test methylation status ZAR1 and SFRP4 regions potentials biomarkers for diagnosis preneoplastic neoplastic lesions cervix.Materials methods: Cytobrush samples were evaluated by Methylation specific PCR (MSP) quantitative MSP (qMSP).Results: frequency increased the grade lesion differences between normal cervical (CC) are statistically significant (p < 0.0001). qMSP...

Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen based on carboplatin (CBDCA). However, CBDCA resistance the main obstacle to better prognosis. An vitro drug-resistant cell model would help understanding molecular mechanisms underlying this drug-resistance phenomenon. aim study was characterize cellular and changes induced CBDCA-resistant ovarian line A2780. selection strategy dose-per-pulse method using concentration...

Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some lesions have not fully responded this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant cancer. In study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, death, reactive oxygen...

Photodynamic therapy (PDT) treats nonmelanoma skin cancer. PDT kills cells through reactive oxygen species (ROS), generated by interaction among cellular O

Aberrant DNA methylation is a hallmark of many cancers. Currently, there are four intrinsic molecular subtypes in breast cancer (BC): Luminal A, B, Her2-positive, and triple negative (TNBC). Recently, The Cancer Genome Atlas (TCGA) project has revealed that have higher levels genome-wide may be result Estrogen/Estrogen receptor α (E2/ERα) signaling pathway activation. In this study, we analyze promoter CpG-island (CGIs) the Reprimo (RPRM) gene cancers (n = 77), cell lines 38), normal tissue...

Gastric cancer (GC) is a significant cancer-related cause of death worldwide. The most used chemotherapeutic regimen in GC based on platinum drugs such as cisplatin (CDDP). However, CDDP resistance reduces advanced survival. In vitro drug-resistant cell model would help the understanding molecular mechanisms underlying this drug-resistance phenomenon. aim study was to characterize new models CDDP-resistant lines (AGS R-CDDP and MKN-28 R-CDDP) obtained through stepwise increasing drug doses...

Frequency of Chlamydia trachomatis infection in a group women from Region Araucanía, ChileBackground: is the most commonly reported sexually transmitted bacterial worldwide.Between 70 and 90% are asymptomatic, however, untreated persistent infections can lead to development urethritis, pelvic inflammatory disease, infertility ectopic pregnancy.Aims: To determine C. frequency Chile, using quantitative real time PCR (qPCR) compare usefulness endocervical urine samples for detection.Methods: 87...

Human T-cell lymphotropic virus type 1 (HTLV-1) is a globally-spread virus. It estimated that there are about 5–10 million infected people in the world. HTLV endemic Chile, with higher seroprevalence among indigenous people. However, little known HTLV-1 genetic diversity, its introduction and dispersion this country. To gain insights into these issues, phylogenetic dating analysis was conducted based on Chilean closed related long terminal repeat sequences. The time tree reconstruction...

The Epstein–Barr virus (EBV) is a globally dispersed pathogen involved in several human cancers of B-cell and non-B-cell origin. EBV has been classified into EBV-1 EBV-2, which have differences their transformative ability. can transform B-cells LCL more efficiently than EBV-2 preferentially infects T-cell lymphocytes. EBNA3A oncoprotein transcriptional regulator host cell genes, required order to B-cells. six peptide motifs called nuclear localization signals (NLSs) that ensure...

Introducción: El virus del papiloma humano de alto riesgo (VPH-AR) es responsable cáncer cuello uterino y sus lesiones preneoplásicas. Los genotipos VPH16 VPH18 son los más frecuentes en este cáncer. La integración VPH-AR el genoma la célula hospedera crucial carcinogénesis cervical, pero etapa que ocurre población chilena incierta.

Abstract New biomarkers are needed to improve cervical cancer screening technologies, which mostly based on cytological examination and HPV detection. However, PAP-smear has a low-sensitivity detect low grade squamous intraepithelial lesions not all infected women will develop preneoplastic or neoplastic lesions. Previous results of our group showed that genes Gen Z (patent pending), CDH1 MEGF9 could be hypermethylated in normal epithelia. The aim this study was determinate if promoter...

Abstract Introduction: Photodynamic therapy (PDT) is a treatment for non melanoma skin cancer. PDT kills cells through reactive oxygen species (ROS), produced by interaction between cellular O2 and an excited photosensitizer (PS) specific light. One of the most used PS in dermatology Protoporphyrin IX (PpIX) its precursor methyl aminolevulinate (MAL). MAL enters into converted to PpIX enzymes heme group synthesis pathway. The insertion ferrous iron forms molecule, which has no action. A...

Abstract Introduction: Cervical Cancer (CC) is an important heath problem in developing countries. The silencing of tumor suppressor genes (TSG) could be implied cervical carcinogenesis. Methylation microarray showed aberrant hypermethylation FKBP6, a potential TSG gen CC. aim this study was to characterizer FKBP6 role Materials and methods: ECT1 E6/E7 (immortalized normal squamous epithelia cell line) three CC lines: SiHa, C-4I C-33A were cultivated for experiments. expression determined by...

Abstract Background: Reprimo, a highly glycosylated protein, is new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and putative tumor suppressor gene frequently silenced via methylation its promoter region in several malignances. The aim this study was to characterize epigenetic inactivation biological function BC lines. Methods: correlation between Reprimo loss mRNA expression assessed six breast cancer lines by specific PCR (MSP), 5-Aza-2′-deoxycytidine...

Abstract Background: In Cervical Cancer (CC) the role of HPV is fundamental; however, not all infected women will develop this disease. Therefore, other mechanisms, such as silencing tumor suppressor genes (TSG), could be implied in cervical carcinogenesis. a previous study, we constructed methylation microarrays, where found promoter aberrant hypermethylation ZNF516 gen CC, postulating it TSG candidate. The aim study was to characterizer carcinogenesis and cell cycle. Materials methods:...

La Sífilis Congénita es una enfermedad que va en incremento a pesar de los controles, estímulos y la educación sexual brindada población el objetivo establecer prevalencia mujeres embarazadas acudieron para hacer sus controles prenatales al Hospital Villa Pagador. Se realizó un estudio tipo retrospectivo, descriptivo corte transversal. El grupo poblacional consiste hicieron su control laboratorial antes las 20 semanas gestación. Durante 2015 se laboratorio 972 mujeres, 7 resultaron positivo,...

Abstract Cellular FLICE-like inhibitory protein (c-FLIP) is a caspasa-8/10 homolog catalytically inactive that interferes with the efficient formation of DISC. There are three isoforms this protein: c-FLIPL (long) 55kDa, c-FLIPS (short) 26kDa y c-FLIPR (Raji) 24kDa. and functions have been well established: both can block extrinsic pathway apoptosis by inhibiting procaspasa-8 activation in However, function still remains unclear. When overexpressed, as some cancers, has an anti-apoptotic...

Abstract Human papillomavirus (HPV) is the most frequent microorganism sexually transmitted and causal agent of cervical carcinoma were it could be found in 99.7% cases. The detection HPV en women with or without lesions cervix generally performed biopsies cytobrush, which are invasive samples; therefore many patients do not attend to health care get test. aim this study was develop a technique capable detect genotype urine samples squamous intraepithelial cervix. For study, 87 from normal...

Introduction Gastric cancer (GC) is an important public health problem in Chile, because constitutes the first cause of death for man and third women. Most GC patients are diagnosed at advanced stages where surgery not effective. In these cases, treatment mainly based chemotherapy with Cisplatin (CDDP) or 5-fluorouracil (5-FU). However, tumour cells usually develop resistance, increasing rate recurrence. The establishment drug resistant cell lines could serve as initial screen agents that...

Introduction Colorectal cancer (CRC) is an important public health problem worldwide. In Chile, CRC the fourth most frequent and its incidence rising. Sporadic results from accumulation of both acquired genetic epigenetic changes that transform normal glandular epithelium into invasive adenocarcinoma. DNA methylation has role in colon carcinogenesis also been found involved metastasis pathways CRC. However, there are no studies analysed whole genome search specific methylated genes CRC,...