A5031063672- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Mechanisms and Therapy
- Cervical Cancer and HPV Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Skin Protection and Aging
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- Nanoplatforms for cancer theranostics
- Viral-associated cancers and disorders
- Photodynamic Therapy Research Studies
- Gut microbiota and health
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- COVID-19 and Mental Health
- Peptidase Inhibition and Analysis
- Laser Applications in Dentistry and Medicine
- Lymphoma Diagnosis and Treatment
- Hepatitis B Virus Studies
- Infrared Thermography in Medicine
Universidad de La Frontera
2015-2025
Millennium Institute on Immunology and Immunotherapy
2023-2025
Center For Biomarker Research In Medicine
2023
Medical University of Graz
2023
Abstract The Epstein-Barr virus (EBV) infects more than 90% of the human population, playing a key role in origin and progression malignant non-malignant diseases. Many attempts have been made to classify EBV according clinical or epidemiological information; however, these classifications show frequent incongruences. For instance, they use small subset genes for sorting strains but fail consider enormous genomic variability abundant recombinant regions present genome. These could lead...
Gastric cancer (GC) is a significant cancer-related cause of death worldwide. GC's most used chemotherapeutic regimen based on platinum drugs such as cisplatin (CDDP). However, CDDP chemoresistance reduces the survival rate advanced GC. The immune C-C chemokine receptor type 5 (CCR5) have been proposed pivotal factor in progression since its blockade has linked with antineoplastic effects tumor cell proliferation; nevertheless, role GC not elucidated. This study aimed to determine induced by...
Gallbladder cancer (GBC) is a highly fatal disease with poor prognosis and few therapeutic alternatives. The molecular mechanisms involved in the pathogenesis of GBC remain poorly understood. vascular endothelial growth factor A (VEGF-A) potent proangiogenic agent carcinogenesis many human tumors an attractive target for therapy. We characterized VEGF-A expression advanced its relation to clinicopathologic features. was examined by immunohistochemistry tissue microarrays containing 224...
Background: Gallbladder carcinoma is a highly malignant tumor and public health problem in some parts of the world. It characterized by poor prognosis its resistance to radio chemotherapy. There an urgent need develop novel therapeutic alternatives for treatment gallbladder carcinoma. The mammalian target rapamycin (mTOR) signaling pathway activated about 50% human malignancies, role has previously been suggested. In present study, we investigated phosphorylation status mTOR substrate p70S6K...
Colorectal cancer is a heterogeneous disease caused by both genetic and epigenetics factors. Analysing DNA methylation changes occurring during colorectal progression metastasis formation crucial for the identification of novel epigenetic markers patient prognosis. Genome-wide sequencing paired samples colon (normal adjacent, primary tumour lymph node metastasis) showed global hypomethylation CpG island (CGI) hypermethylation tumours compared to normal. In we observed high non-CGI regions...
Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen based on carboplatin (CBDCA). However, CBDCA resistance the main obstacle to better prognosis. An vitro drug-resistant cell model would help understanding molecular mechanisms underlying this drug-resistance phenomenon. aim study was characterize cellular and changes induced CBDCA-resistant ovarian line A2780. selection strategy dose-per-pulse method using concentration...
Treatment options for advanced gallbladder cancer (GBC) are scarce and usually rely on cytotoxic chemotherapy, but the effectiveness of any regimen is limited recurrence rates high. Here, we investigated molecular mechanisms acquired resistance in GBC through development characterization two gemcitabine-resistant cell sublines (NOZ GemR TGBC1 GemR). Morphological changes, cross-resistance, migratory/invasive capabilities were evaluated. Then, microarray-based transcriptome profiling...
Reprimo (RPRM), a highly glycosylated protein, is new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and putative tumor suppressor gene frequently silenced via methylation its promoter region in several malignances. The aim this study was to characterize epigenetic inactivation biological function BC lines.The correlation between RPRM loss mRNA expression assessed six breast cancer lines by specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment RT-PCR assays....
Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some lesions have not fully responded this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant cancer. In study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, death, reactive oxygen...
Photodynamic therapy (PDT) treats nonmelanoma skin cancer. PDT kills cells through reactive oxygen species (ROS), generated by interaction among cellular O
Human papillomavirus (HPV) is the etiological factor for cervical cancer and its precursor lesions. The characterization of HPV genotypes in preneoplastic lesions could establishes effectiveness vaccination plan Chilean population. aim this study was to determine frequency a group women including screening program public health care system Chile. We analyzed 985 smears samples from with different histological diagnosis, attending Temuco-Chile between 2004 2012, detect genotypes, through PCR...
Aberrant DNA methylation is a hallmark of many cancers. Currently, there are four intrinsic molecular subtypes in breast cancer (BC): Luminal A, B, Her2-positive, and triple negative (TNBC). Recently, The Cancer Genome Atlas (TCGA) project has revealed that have higher levels genome-wide may be result Estrogen/Estrogen receptor α (E2/ERα) signaling pathway activation. In this study, we analyze promoter CpG-island (CGIs) the Reprimo (RPRM) gene cancers (n = 77), cell lines 38), normal tissue...
Gastric cancer (GC) is a significant cancer-related cause of death worldwide. The most used chemotherapeutic regimen in GC based on platinum drugs such as cisplatin (CDDP). However, CDDP resistance reduces advanced survival. In vitro drug-resistant cell model would help the understanding molecular mechanisms underlying this drug-resistance phenomenon. aim study was to characterize new models CDDP-resistant lines (AGS R-CDDP and MKN-28 R-CDDP) obtained through stepwise increasing drug doses...
Background: Aberrant DNA hypermethylation in tumor suppressor genes is a common feature cervical cancer (CC). This abnormal could be used as potential biomarkers for detecting CC non-invasive samples such urine and plasma.Methods: study aimed to evaluate plasma the methylation status of two previously found hypermethylated (ZAR1 SFRP4) assesses their diagnostic value. Thus, was measured 171 paired taken from women with non-lesions [60], low-grade squamous intraepithelial lesions (L-SIL)...
Introducción: El virus del papiloma humano de alto riesgo (VPH-AR) es responsable cáncer cuello uterino y sus lesiones preneoplásicas. Los genotipos VPH16 VPH18 son los más frecuentes en este cáncer. La integración VPH-AR el genoma la célula hospedera crucial carcinogénesis cervical, pero etapa que ocurre población chilena incierta.
Gastric cancer (GC) is a complex and highly variable disease, ranking among the top five cancers diagnosed globally, leading cause of cancer-related deaths. Emerging from stomach lining cells amid chronic inflammation, it often advances to preneoplastic stages. Late-stage diagnoses treatment challenges highlight critical need for early detection innovative biomarkers, motivating this study's focus on identifying theranostic markers through gene ontology analysis. By exploring deregulated...
242 Background: Gallbladder cancer (GBC) is a highly malignant tumor usually diagnosed at advanced stages and characterized by poor prognosis. Effective therapeutic strategies are urgently needed to improve the prognosis of GBC patients. Our objective was analyze expression mTOR/p70S6K pathway in primary tumors cell lines, evaluate effect mTOR inhibitors vitro vivo models GBC. Methods: The signaling components examined immunohistochemistry chronic cholecystitis (CC), Western blot eight...