A5014501808- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Amyotrophic Lateral Sclerosis Research
- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Mesenchymal stem cell research
- Nuclear Receptors and Signaling
- Bone and Joint Diseases
- Heat shock proteins research
- Cellular Mechanics and Interactions
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- Neurogenetic and Muscular Disorders Research
- NF-κB Signaling Pathways
- Neurogenesis and neuroplasticity mechanisms
- Adenosine and Purinergic Signaling
- Inflammatory mediators and NSAID effects
- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Gut microbiota and health
- Gastrointestinal motility and disorders
- Nanoparticle-Based Drug Delivery
University of Genoa
2012-2023
Istituto di Nanotecnologia
2022
Dimethyl fumarate (DMF), recently approved as an oral immunomodulatory treatment for relapsing-remitting multiple sclerosis (MS), metabolizes to monomethyl (MMF) which crosses the blood-brain barrier and has demonstrated neuroprotective effects in experimental studies. We postulated that MMF exerts through modulation of microglia activation, a critical component neuroinflammatory cascade occurs neurodegenerative diseases such MS. To ascertain our hypothesis define mechanistic pathways...
Abstract Mesenchymal stem cells (MSC) display a remarkable ability to modulate the immune response and protect central nervous system mainly through release of soluble factors in paracrine fashion, affecting functional behavior tissues. Here we investigated effect interaction between MSC microglia vitro, dissected molecular cellular mechanisms this crosstalk. We demonstrated that impair activation by inflammatory cues inhibition expression molecules stress-associated proteins. showed...
Abstract Mesenchymal stromal/stem cells (MSCs) are characterized by neuroprotective, immunomodulatory, and neuroregenerative properties, which support their therapeutic potential for inflammatory/neurodegenerative diseases, including multiple sclerosis (MS) amyotrophic lateral (ALS). One mode of action through MSCs exert immunomodulatory effects is release extracellular vesicles that carry proteins, mRNAs, microRNAs (miRNAs), which, once transferred, modify the function target cells. We...
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with no effective cure. Astrocytes display toxic phenotype in ALS and contribute to motoneuron (MN) degeneration. Modulating astrocytes' neurotoxicity can reduce MN death. Our previous studies showed the beneficial effect of mesenchymal stem cell (MSC) administration SOD1G93A mice, but mechanisms are still unclear. We postulated that effects could be mediated by extracellular vesicles (EVs) secreted MSCs. investigated,...
Abstract The 3D complexity of biological tissues and intricate structural-functional connections call for state-of-the-art X-ray imaging approaches to overcome limitations classical imaging. Unlike other techniques, phase-contrast tomography (XPCT) offers a highly sensitive approach investigate different disease-relevant networks at levels ranging from single cell through intact organ. We present here concomitant study the evolution tissue damage inflammation in organs affected by disease...
Monomethyl fumarate (MMF), metabolite of dimethyl (DMF), an immunosuppressive drug approved for the treatment multiple sclerosis (MS), is a potent agonist hydroxycarboxylic acid receptor 2 (HCAR2), eliciting signals that dampen cell activation or lead to inflammation such as skin flushing reaction one main side effects treatment, together with gastrointestinal inflammation. Our aim further understand molecular basis underlying these differential drug. We have used wild-type and HCAR2...
Introduction: In the study of neurodegenerative diseases, possibility to follow fate specific cells or molecules within whole body would be a milestone better understand complex evolution disease mechanisms and monitor effects therapies. The techniques available today do not allow visualization disease-relevant tridimensional biological context at high spatial resolution. Methods: Here we show results from first validation steps novel approach: by combining conjugate nanobodies anti-glial...
Abstract Background Mesenchymal stromal/stem cells (MSCs) are characterized by neuroprotective, immunomodulatory, and neuroregenerative properties, which support their therapeutic potential for neurodegenerative diseases driven microglia-associated inflammation, such as amyotrophic lateral sclerosis (ALS). One mode of action through MSCs exert immunomodulatory effects is the release extracellular vesicles, including exosomes, that carry proteins, mRNAs, microRNAs (miRNAs), which, once...
Abstract The 3D complexity of biological tissues and the intricate structural-functional connections call for modern X-ray imaging approaches to overcome limitations classical imaging. Unlike other techniques, phase-contrast tomography (XPCT) offers an unprecedented hierarchical approach investigate different disease-relevant networks at levels ranging from single cell through intact organ as a whole. We study evolution tissue damage inflammation in organs affected by disease murine model...