Current dorsal skin flap window chambers with flat glass windows are compatible optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence (LSFM) performs best a cylindrical or spherical sample located between its two 90° objectives when all materials have the same index of refraction (n). A modified chamber domed viewing made from fluorinated ethylene propylene (FEP), n similar to water tissue, was designed. In vitro imaging collagen gels...

We present the results of first feasibility and safety study a novel multi-modality falloposcope, in 19 volunteers. The falloposcope incorporated multispectral fluorescence imaging (MFI) optical coherence tomography (OCT) for evaluation fallopian tubes (FT). Nineteen females undergoing elective salpingectomy were recruited this IRB-approved study. During 15-min pause standard care surgery, was inserted under hysteroscopic guidance advanced into FTs. furthest cannulated segment (proximal,...

382 Background: Individuals at high risk for gastric cancer may be recommended to undergo risk-reducing gastrectomy, which leads life-long morbidity. Surveillance with endoscopy can an appropriate and effective option avoiding unnecessary surgery. Gastric is commonly characterized by changes in tissue architecture increased stiffness, but visualization of these alterations challenging white light methods. We utilized optical coherence tomography (OCT), a non-destructive imaging modality that...

With early detection, 5-year survival rates for ovarian cancer exceed 90%, yet no effective screening method exists. Emerging consensus suggests over 50% of the most lethal form disease originates in fallopian tube. Twenty-eight women undergoing oophorectomy or debulking surgery provided informed consent use surgical discard tissue samples multispectral fluorescence imaging. Using multiple ultraviolet and visible excitation wavelengths emissions bands, 12 6 reflectance images 47 31 tube were...

Ovarian cancer has the lowest survival rate among all gynecologic cancers due to predominantly late diagnosis. Optical coherence tomography (OCT) been applied successfully experimentally image ovaries in vivo; however, a robust method for analysis is still required provide quantitative diagnostic information. Recently, texture proved be useful tool tissue characterization; unfortunately, existing work scope of OCT ovarian imaging limited only analyzing 2D sub-regions data, discarding...

Second-harmonic-generation (SHG) imaging of mouse ovaries ex vivo was used to detect collagen structure changes accompanying ovarian cancer development. Dosing with 4-vinylcyclohexene diepoxide and 7,12-dimethylbenz[a]anthracene resulted in histologically confirmed cases normal, benign abnormality, dysplasia, carcinoma. Parameters for each SHG image were calculated using the Fourier transform matrix gray-level co-occurrence (GLCM). Cancer versus normal all other diagnoses showed greatest...

High grade serous ovarian cancer is the most deadly gynecological cancer, and it now believed that cases originate in fallopian tubes (FTs). Early detection of could double 5-year survival rate compared with late-stage diagnosis. Autofluorescence imaging can detect serous-origin precancerous cancerous lesions

Optical coherence tomography (OCT) can provide new insight into disease progression and therapy by enabling nondestructive, serial imaging of in vivo cancer models. In previous studies, we have shown the utility endoscopic OCT for identifying adenomas azoxymethane-treated mouse model colorectal tracking over time. Because improved speed made possible through Fourier domain imaging, three-dimensional entire colon is possible. Increased amounts data facilitate more accurate classification...

Abstract Background and Objective Ovarian cancer has an extremely high mortality rate resulting from poor understanding of the disease. In order to aid disease etiology progression, we identify endogenous fluorophores present in a mouse model ovarian describe changes fluorophore abundance distribution with age Study Design/Materials Methods A was created by dosing 4‐vinylcyclohexene diepoxide, which induces follicular apoptosis (simulating menopause), 7,12‐dimethylbenz[ ]anthracene, known...

Identification of the early microscopic changes associated with ovarian cancer may lead to development a diagnostic test for high-risk women. In this study we use optical coherence tomography (OCT) and multiphoton microscopy (MPM) (collecting both two photon excited fluorescence [TPEF] second harmonic generation [SHG]) image mouse ovaries in vivo at multiple time points. We demonstrate feasibility imaging during long-term survival identify tumor development. These include alterations tissue...

Most cases of high-grade serous ovarian carcinoma originate as tubal intraepithelial (STIC) lesions in the fallopian tube epithelium (FTE), enabling early endoscopic detection.

The development of colorectal cancer in the azoxymethane-induced mouse model can be observed by using a miniaturized optical coherence tomography (OCT) imaging system. This system is uniquely capable tracking disease over time, allowing for monitoring morphological changes distal colon due to tumor and presence lymphoid aggregates. By genetically engineered models deficient Interleukin 6 (IL-6) Smad family member 3 (Smad3), role inflammation on immune elucidated. Smad3 knockout mice develop...

Sulindac is an NSAID that can provide effective chemoprevention for colorectal cancer. In this study, alternative dosing regimens of sulindac were evaluated their effectiveness in the azoxymethane-treated A/J mouse model High-resolution endoscopic optical coherence tomography was utilized to time-serially measure tumor number and burden distal colon as biological endpoints. Four treatment groups studied: (i) daily 20 weeks (sulindac-daily); (ii) 2 weeks, then no cycle repeated 5 times...

Optical imaging methods have the potential to detect ovarian cancer at an early, curable stage. has disadvantage that high resolution techniques require access tissue of interest, but miniature endoscopes traverse natural orifice reproductive tract, or ovaries and fallopian tubes through a small incision in vagina wall, can provide minimally-invasive solution. We imaged both rodent human with variety endoscope-compatible modalities. The recent development fiber-coupled femtosecond lasers...

Mueller polarimetric imaging in dark-field observation shows a contrast enhancement between healthy and cancerous human colon tissue some reports. We have developed Mueller-matrix microscope system that combines polarization illuminator with an polarimeter to measure the characteristics of scattered light from samples. A multichannel source permits acquisition multispectral matrices sample. The wavelength state selections are automated, as is matrix measurement. perform fast, stable...

Falloposcopy is the endoscopic examination of fallopian tubes, which are challenging to access due their deep body location, small opening from uterus, and lumen filled with plicae. We others have developed endoscopes that inserted through uterus guided by a hysteroscope into tubal ostium. To better understand how utilize these either as standalone devices or in concert everting delivery balloons, preliminary study anatomy mechanical behavior was performed ex vivo on porcine human tubes....

A reflectance volume holographic imaging (VHI) endoscope has been designed for simultaneous in vivo of surface and subsurface tissue structures. Prior utilization VHI systems limited to ex imaging. The system presented this work is laparoscopic use. It consists a probe section that relays light from the sample handheld unit contains microscope. constructed gradient index (GRIN) lenses form 1:1 relay image collection. an outer diameter 3.8 mm capable achieving 228.1 ?? lp / resolution with...

The NSAID sulindac has been successfully used alone or in combination with other agents to suppress colon tumorigenesis patients genetic predisposition and also showed its efficacy prevention of sporadic adenomas. At the same time, some experimental clinical reports suggest that a mutant K-RAS oncogene may negate antitumor efficacy. To directly assess activity at suppressing premalignant lesions carrying mutation, we utilized novel mouse model an inducible colon-specific expression K-ras...

Ovarian cancer has the lowest survival rate among all gynecologic cancers predominantly due to late diagnosis. Early detection of ovarian can increase 5-year rates from 40% up 92%, yet no reliable early techniques exist. Optical coherence tomography (OCT) is an emerging technique that provides depth-resolved, high-resolution images biological tissue in real-time and demonstrates great potential for imaging tissue. Mouse models are crucial quantitatively assess diagnostic OCT imaging;...

A wavelength-coded volume holographic imaging (WC-VHI) endoscope system capable of simultaneous multifocal is presented. The images light from two depths separated by 100 μm in a tissue sample using axial chromatic dispersion gradient index probe combination with light-emitting diode sources and multiplexed hologram to separate the images. This different previous VHI systems that it uses planar gratings does not require curved gratings. results improved lateral resolution 228.1 322.5 lp/mm....

Ovarian cancer has the lowest survival rate among all gynecologic cancers due to predominantly late diagnosis. Early detection of ovarian can increase 5-year rates from 40% up 92%, yet no reliable early techniques exist. Optical coherence tomography (OCT) is an emerging technique that provides depthresolved, high-resolution images biological tissue in real time and demonstrates great potential for imaging tissue. Mouse models are crucial quantitatively assess diagnostic OCT imaging; however,...

Background and Objectives To determine the efficacy of targeted fluorescent biomarkers multiphoton imaging to characterize early changes in ovarian tissue with onset cancer. Study Design/Materials Methods A transgenic TgMISIIR‐TAg mouse was used as an animal model for Mice were injected dyes bind folate receptor α, matrix metalloproteinases, integrins. Half mice treated 4‐vinylcyclohexene diepoxide (VCD) simulate menopause. Widefield fluorescence (WFI) ovaries oviducts conducted at 4 8 weeks...