A5040694447- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Sepsis Diagnosis and Treatment
- Bacterial Infections and Vaccines
- Salmonella and Campylobacter epidemiology
- Neonatal Respiratory Health Research
- Mycotoxins in Agriculture and Food
- Infections and bacterial resistance
- Neonatal and Maternal Infections
- Cystic Fibrosis Research Advances
- Respiratory Support and Mechanisms
- Escherichia coli research studies
- Nosocomial Infections in ICU
- Fungal Infections and Studies
- Neutropenia and Cancer Infections
- Respiratory viral infections research
- Antifungal resistance and susceptibility
- Hematological disorders and diagnostics
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
Bambino Gesù Children's Hospital
2023-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024-2025
Università Cattolica del Sacro Cuore
2020-2024
A mong molecular assays currently developed for detection and identification of pathogens (and their antimicrobial resistance genes) in positive blood cultures (BCs) (1), the BioFire FilmArray culture (BCID) panel (bioMérieux, Marcy l'Etoile, France), a multiplex PCR assay with ,2 min hands-on time an ;1-h turnaround time, allows syndromic diagnosis bloodstream infection (BSI) (2, 3).Previously, could identify 24 etiological agents BSI (11 Gram-negative bacteria, 8 Gram-positive 5 yeast...
Background: Bloodstream infections affect up to 20% of pediatric cancer patients receiving intensive care, contributing significantly morbidity and mortality, with infection-related mortality rates reported be as high 16%. Methods: The identification microorganisms directly from whole blood is difficult due several factors, such interference host genomic material, low bacterial load, the endogenous components exogenous substances, which can interfere process. Nevertheless, rapid microbial...
Background/Objectives: Paediatric osteoarticular infections (POAIs) present unique diagnostic and therapeutic challenges. Microbiological culture (MC) is typically time-consuming lacks sensitivity, especially when patients have received antibiotics. The BIOFIRE® Joint Infection Panel (BJIP) a syndromic molecular assay for the direct identification of most pathogens causing POAIs. Methods: We evaluated BJIP in 17 synovial fluids, then, we retrospectively assessed its utility 93 off-label...
Bacterial pneumonia is a challenging coronavirus disease 2019 (COVID-19) complication for intensive care unit (ICU) clinicians. Upon its implementation, the FilmArray plus (FA-PP) panel's practicability both diagnosis and antimicrobial therapy management of bacterial was assessed in ICU patients with COVID-19. Respiratory samples were collected from who mechanically ventilated at time etiology resistance determined using standard-of-care (culture susceptibility testing [AST]) FA-PP panel...
The aim of this study was to characterize COVID-19 (SARS-CoV-2-infected) patients who develop bloodstream infection (BSI) and assess risk factors associated with in-hospital mortality. We conducted a retrospective observational adult admitted for ≥48 h large Central Italy hospital (1 March 31 May 2020) had or not survived at discharge. included only having blood cultures drawn other inclusion criteria satisfied. Kaplan–Meier survival Cox regression analyses were performed 293 studied, 46...
Invasive group A streptococci infections are increasing worldwide, mainly due to the emm1 lineage M1 UK emergence. Although this variant has recently been described in adult Italian patients, its circulation children not yet established. Characterizing by whole genome sequencing 6 invasive strains isolated between 2022 and 2023, we highlighted pediatric patients Italy.
In the present study, in vitro activity of sulbactam–durlobactam (SUL–DUR) combination was evaluated against 141 carbapenem-resistant A. baumannii (CRAb) clinical strains collected from six Italian laboratories. Over half (54.6%) these isolates were resistant to colistin. The SUL–DUR active CRAb with MIC50 and MIC90 values 0.5 mg/L 4 mg/L, respectively. Only eleven MIC ranging 8 128 mg/L. exhibited several antimicrobial resistance genes (ARGs) such as blaOXA-20, blaOXA-58, blaOXA-66,...
Background: Infant meningitis, particularly caused by Escherichia coli, remains a life-threatening condition, especially in premature and low-weight infants. Infections of the central nervous system can be fatal, necessitating prompt diagnosis appropriate treatment. Acute infections various pathogens, including E. often present with similar clinical symptoms. The rapid identification pathogens their antimicrobial resistance mechanisms is critical for timely effective We report case an...
While echinocandins remain the preferred antifungal therapy for candidemia, bloodstream infections caused by C. auris , glabrata or, at a lesser extent, parapsilosis may be difficult to treat with these agents. This is in view of high propensity above-mentioned species develop resistance or tolerance during treatment.
Bacterial infections caused by multidrug-resistant (MDR) Gram-negatives are of great concern worldwide, as they frequently associated with high mortality and morbidity rates. To date, two cases VIM-2 metallo-β-lactamase (MBL)-producing Pseudomonas putida bacteremia have been ever reported in France Spain between 2004 2010. Here, we present the first case VIM-1-like-producing P. isolated blood culture collected from an oncohematological pediatric patient admitted to Bambino Gesù Children's...
This study aimed to assess the comparability of in vitro susceptibility testing methods ceftazidime-avibactam (CZA) and ceftolozane-tazobactam (C/T). Meropenem-resistant and/or carbapenemase-producing clinical isolates Enterobacterales (Enterobacteriaceae) Pseudomonas aeruginosa were tested by both bioMérieux ETEST VITEK-2 AST-N397 card compared with a Micronaut AST-system broth microdilution (BMD) method. CZA C/T MICs interpreted using EUCAST breakpoints. Of 153 Enterobacteriaceae isolates,...
Cystic fibrosis (CF) is a life-threatening genetic disease characterised by chronic lung infections sustained opportunistic pathogens such as
(1) Background: The literature reports a low risk of serious bacterial infections (SBIs) in febrile infants presenting with bronchiolitis or respiratory syncytial virus infection, but current microbiological techniques have higher accuracy. (2) Methods: We assessed the SBIs neonates and from 2021 to 2023. also evaluated C-reactive protein, procalcitonin, leukocyte values. (3) Results: included 242 infants. Blood cultures (BCs) were performed 66/242 patients, positivity rate 9.1% (including one BC