A5101798643- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Renal and related cancers
- Pharmacological Effects of Natural Compounds
- Circular RNAs in diseases
- Axon Guidance and Neuronal Signaling
- Lipid metabolism and disorders
- Apelin-related biomedical research
- Pluripotent Stem Cells Research
- Hydrology and Drought Analysis
- Radiation Therapy and Dosimetry
- Nuclear Receptors and Signaling
- Medicinal Plants and Bioactive Compounds
- Genetics and Neurodevelopmental Disorders
- NF-κB Signaling Pathways
- FOXO transcription factor regulation
- Kruppel-like factors research
- Cancer therapeutics and mechanisms
- Eicosanoids and Hypertension Pharmacology
- Autophagy in Disease and Therapy
- Effects of Radiation Exposure
- Microbial Community Ecology and Physiology
- Neurological Disease Mechanisms and Treatments
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
Shanghai East Hospital
2025
Nanjing University
2024
Nanjing General Hospital of Nanjing Military Command
2024
Zhongnan Hospital of Wuhan University
2015-2023
Wuhan University
2015-2023
China Agricultural University
2023
Huazhong University of Science and Technology
2004-2021
Tongji Hospital
2015-2021
Union Hospital
2004-2021
Wuhan Puai Hospital
2020-2021
Circular RNAs (circRNAs) are a unique class of endogenous which could be used as potential diagnostic and prognostic biomarkers many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging uncover age-related variations that potentially reflect decreased oocyte competence. CircRNAs GCs from vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) advanced (AA, ≥ 38 were profiled by microarray, validated 20 paired samples. The...
Atherosclerosis is a lipid-driven disease in which macrophage foam cells play critical role by increasing vascular lipid accumulation and contributing to plaque instability. Ginsenoside Rb1 (Rb1), the most abundant active component of ginseng, was found potentially promote metabolism attenuate accumulation. However, underlying mechanisms remain unclear. In this study, effects on stability were investigated both vitro vivo using primary peritoneal macrophages isolated from C57BL/6 mice...
Follicular atresia is the main process responsible for loss of follicles and oocytes from ovary, it root cause ovarian aging. Apoptosis granulosa cells (GCs) cellular mechanism follicular in mammals. Recent advances have highlighted fundamental roles EGR1 age-related diseases via induction apoptosis. In present study, we found that expression was significantly increased aged mouse ovaries compared with young ovaries. Immunohistochemical analysis revealed strongly positive staining atretic...
Atherosclerosis is a lipid-driven, systemic immune-inflammatory disease characterized by the accumulation of plaque within arterial walls. Plaque regression can occur following appropriate treatment interventions. Optical coherence tomography (OCT), high-resolution imaging modality, frequently employed to assess morphology. This study aims explore correlation between characteristics identified using OCT, particularly macrophage infiltration, and subsequent regression. In this retrospective...
Abstract As a fundamental aging mechanism, cellular senescence causes chronic inflammation via the senescence‐associated secretory phenotype (SASP). Theca‐interstitial cells are an essential but little‐studied component of follicle development in ovarian microenvironment. In present study, we observed significant theca‐interstitial and secretion chemokine (C‐C motif) ligand 5 (CCL5) by these during aging. Furthermore, aimed to investigate whether how (SASP)‐associated CCL5 may be involved...
Early menopause and infertility often occur in female cancer patients after chemotherapy (CTx). For these patients, oocyte/embryo cryopreservation or ovarian tissue is the current modality for fertility preservation. However, above methods are limited long-term protection of function, especially preservation (very few females with have achieved pregnancy cryopreserved eggs until now). In addition, subject to their scope (females no husband prepubertal mature oocytes). Thus, many who suffer...
Vulvovaginal atrophy (VVA) is a common menopause-related symptom affecting more than 50% of midlife and older women cancer patients whose ovarian function are lost or damaged. Regardless estrogen deficiency, whether other factors such as the gut microbiota play role in VVA have not been thoroughly investigated. To this end, we performed ovariectomy on 12-weeks' old mice follow-up at 4 weeks after ovariectomy, observed atrophied vagina an altered ovariectomized mice.. We further fecal...
Female germline stem cells (FGSCs) or oogonial (OSCs) have the capacity to generate newborn oocytes and thus open a new door fight ovarian aging female infertility. However, production identification of OSCs are difficult for investigators. Rare amount these in ovary results failure acquisition OSCs. Furthermore, oocyte formation by vivo was usually confirmed using tissue sections immunofluorescence immunohistochemistry previous studies. STO MEF feeder derived from mouse, not human. In our...
Abstract The primordial follicle assembly, activation and the subsequent development are critical processes for female reproduction. A limited number of follicles activated to enter growing pool each wave, progressively diminishes over a woman's life‐time. remaining represents ovarian reserve. Identification functional investigation factors involved in follicular initial recruitment will be great significance understanding reproduction process ageing. In this study, we aimed study whether...
Nuclear receptor subfamily 2 group E member 1 (Nr2e1) has been regarded as an essential regulator of the growth neural stem cells. However, its function elsewhere is unknown. In present study, we generated Nr2e1 knockdown MIN6 cells and studied whether affected basal beta cell functions such proliferation, death, insulin secretion. We showed that in resulted increased sensitivity to lipotoxicity, decreased a partial G0/G1 cell-cycle arrest, higher rates apoptosis. Moreover, deficiency...
Mammalian oocytes go through a long and complex developmental process, while acquiring the competencies that are required for fertilization embryogenesis. Recent studies revealed communication between granulosa cells (GCs) is critical process female follicle development. In current study, we aimed to study whether how semaphorin 6C (Sema6c) regulated cell junctions GCs in mice preantral follicles. The attenuation of SEMA6C expression by siRNA decreased cell-cell accelerated atresia vitro....
There are about 1-2 million follicles presented in the ovary at birth, while only around 1000 primordial left menopause. The ovarian function also decreases parallel with aging. Folliculogenesis is vital for function, no matter synthesis of female hormones or ovulation, yet mechanisms its changing increasing age not fully understood. Early follicle growth up to large preantral stage independent gonadotropins rodents and relies on intraovarian factors. To further understand age-related...
The involvement of circular RNAs (circRNAs) in the development cancers has attracted much interest. This study aimed to determine role circ_0000376 non-small cell lung cancer (NSCLC) and provide a new mechanism.The expression circ_0000376, miR-488-3p bromodomain containing 4 (BRD4) mRNA was measured by quantitative real-time PCR (qPCR). Cell behaviors, including proliferation, invasion, migration, apoptosis cycle progression were investigated using counting kit-8 (CCK-8) assay colony...
Phytoplankton and bacteria play key roles in material cycling their consequent eco-functions lakes, which are threatened by anthropogenic pressures, especially agricultural activities, which, the watershed, effective changing composition hydrodynamic conditions of lake through input water withdrawal. This process theoretically changes interaction assembly pattern microorganisms, important factors driving structural functional evolution ecological communities lakes. In this research,...
Objective To study the low dose hyper-radiosensitivity in human lung cancer cell line A549,and its possible mechanisms.Methods Exponentially growing A549 cells were irradiated with 60Co γ-rays at doses of 0-2 Gy.Together flow cytometry for precise sorting,cell survival fraction was measured by mean conventional colony-formation assay.ATM1981 Ser-P protein expression examined Western blot.Apoptosis identified Hoechst 33258 fluorescent staining,and Annexin V-FITC and propidium iodide staining...
To evaluate the clinical effect of gemcitabine and concurrent three-dimensional conformal radiation therapy (3D-CRT) for locally advanced non-small cell lung cancer (NSCLC).From April 2002 to June 2003, 28 patients with inoperable stage III NSCLC were treated 3D-CRT simultaneously. Chemotherapy consisted intravenously 350 mg/m 2 on days 1, 8, 15, 22, 29, 36. was delivered up a total dose 60-64 Gy 2.0 fraction per day, 5 week.The overall response rates primary tumor mediastinum metastatic...
high cholesterol diet intervention on spatial learning performance of middle-aged rats by using the Morris water maze. Materials and methods AnimalsEighty 8-month-old female Sprague-Dawley (weighted 336.95 ± 42.00 g), were obtained from Hubei province center disease control prevention (Wuhan, China).Five housed per cage maintained at a controlled ambient temperature (24 1°C) under diurnal conditions (light-dark: 08:00-20:00) with access to laboratory chow tap ad libitum.Adequate levels...
Real-time polymerase chain reaction(PCR) and methylation-specific PCR were used to test the expression of dopamine receptor D2(DRD2) level DNA methylation by screening 15 cases aldosteroneproducing adenoma(APA) 3 normal adrenal cortex samples.mRNA DRD2 in APA was significantly lower than that cortex.The methylated plays a partial role APA. Key words: Adrenocortical adenoma ; Receptors, D2 Reverse transcriptase reaction; Methylation-specific reaction