A5012417430- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Organ Transplantation Techniques and Outcomes
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Genetic Neurodegenerative Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism, Diabetes, and Cancer
- Alzheimer's disease research and treatments
- Manufacturing Process and Optimization
- Exercise and Physiological Responses
- Protein Structure and Dynamics
- Vascular Procedures and Complications
- Central Venous Catheters and Hemodialysis
- ATP Synthase and ATPases Research
- Veterinary Practice and Education Studies
- MicroRNA in disease regulation
- Circular RNAs in diseases
University of Rochester
2005-2024
Duke University
2019-2024
New York State College of Veterinary Medicine
1991-2024
Cornell University
1991-2024
University of Arkansas at Fayetteville
2014-2023
Beaumont Health
2022-2023
Oakland University
2022-2023
Centro de Ensino Superior de Juiz de Fora
2023
University of California, San Diego
2006-2022
Duke Medical Center
2021
Cancer cachexia is largely irreversible, at least via nutritional means, and responsible for 20-40% of cancer-related deaths. Therefore, preventive measures are primary importance; however, little known about muscle perturbations prior to onset cachexia. associated with mitochondrial degeneration; yet, it remains be determined if degeneration precedes wasting in cancer our purpose was determine cancer-induced tumour-bearing mice.
Biochemical basis of a 24-hour clock Circadian clocks keep organisms in synch with such daily cycles as illumination, activity, and food availability. The circadian cyanobacteria has the necessary period despite its three component proteins having biochemical activities that occur on much faster time scale. Abe et al. focused cyanobacterial KaiC, an adenosine triphosphatase (ATPase) can autophosphorylate autodephosphorylate. slow ATPase activity which is linked to peptide isomerisation,...
Ebolaviruses cause hemorrhagic fever with up to 90% lethality and in fatal cases, are characterized by early suppression of the host innate immune system. One proteins likely responsible for this effect is VP24. VP24 known antagonize interferon signaling binding karyopherin α proteins, thereby preventing them from transporting tyrosine-phosphorylated transcription factor STAT1 nucleus. Here, we report that binds directly, suggesting can suppress at least two distinct branches pathway. also...
Insulin degrading enzyme (IDE) plays key roles in peptides vital type two diabetes, Alzheimer's, inflammation, and other human diseases. However, the process through which IDE recognizes that tend to form amyloid fibrils remained unsolved. We used cryoEM understand both apo- insulin-bound dimeric states, revealing displays a large opening between homologous ~55 kDa N- C-terminal halves allow selective substrate capture based on size charge complementarity. also cryoEM, X-ray crystallography,...
Abstract Background Cancer cachexia occurs in approximately 80% of cancer patients and is a key contributor to cancer‐related death. The mechanisms controlling development tumour‐induced muscle wasting are not fully elucidated. Specifically, the progression underexplored. Therefore, we examined skeletal protein turnover throughout tumour‐bearing mice. Methods Lewis lung carcinoma (LLC) was injected into hind flank C57BL6/J mice at 8 weeks age with tumour allowed develop for 1, 2, 3, or 4...
Neutralizing antibodies prevent Zika infection in nonhuman primates.
Hepatitis C virus (HCV) is a major cause of liver disease, affecting over 2% the world's population. The HCV envelope glycoproteins E1 and E2 mediate viral entry, with being main target neutralizing antibody responses. Structural investigations have produced templates for vaccine design, including conserved CD81 receptor-binding site (CD81bs) that key broadly antibodies (bNAbs). Unfortunately, immunization recombinant E1E2 rarely elicits sufficient levels bNAbs protection. To understand...
Skeletal muscle mitochondrial degeneration is a hallmark of insulin resistance/obesity marked by lost function, enhanced ROS emission, and altered morphology which may be ameliorated physical activity (PA). However, no prior report has examined quality control regulation throughout biogenesis, fusion/fission dynamics, autophagy, permeability transition pore (MPTP) in obesity. Therefore, we determined how each process impacted Western diet (WD)-induced obesity whether voluntary PA alleviate...
Avian H7N9 influenza viruses are group 2 A that have been identified as the etiologic agent for a current major outbreak began in China 2013 and may pose pandemic threat. Here, we examined human H7-reactive antibody response 75 recipients of monovalent inactivated A/Shanghai/02/2013 vaccine. After doses vaccine, majority donors had memory B cells secreted IgGs specific H7 HA, with dominant responses against single HA subtypes, although frequencies ranged widely between donors. We isolated 12...
Abstract Background Muscle atrophy is a common pathology associated with disuse, such as prolonged bed rest or spaceflight, and detrimental health outcomes. There emerging evidence that disuse may differentially affect males females. Cellular mechanisms contributing to the development progression of remain elusive, particularly protein turnover cascades. The purpose this study was investigate initial muscle in male female mice using well‐established model hindlimb unloading (HU). Methods One...
The purpose of this study was to determine the functional results transvenous angioplasty for treatment central venous stenoses in patients with failing upper extremity arteriovenous access. Two hundred consecutive presented threatened access from January 1999 through July 2002. Angiographic evidence stenosis present 35 (18%) (superior vena cava 5, brachiocephalic veins 14, and subclavian 18). Follow-up averaged 873 days date initial intervention. technical success rate 89%. Primary patency...
Several live attenuated rotavirus (RV) vaccines have been licensed, but the mechanisms of protective immunity are still poorly understood. The most frequent human B cell response is directed to internal protein VP6 on surface double-layered particles, which normally exposed only in intracellular environment. Here, we show that canonical antibodies secreted by humans bind such particles and inhibit viral transcription. Polymeric IgA RV mediated an inhibitory effect against virus replication...
Muscle atrophy is a hallmark of cancer cachexia resulting in impaired function and quality life the immediate cause death for 20-40% patients. Multiple microRNAs (miRNAs) have been identified as being involved muscle development atrophy; however, less known specifically on miRNAs cachexia. The purpose this investigation was to examine miRNA profile skeletal induced by uncover potential with catabolic condition. Phosphate-buffered saline (PBS) or Lewis lung carcinoma cells (LLC) were injected...
ABSTRACT Insulin resistant diabetes, currently at epidemic levels in developed countries, begins the skeletal muscle and is linked to altered protein turnover. microRNAs downregulate targeted mRNA translation decreasing amount of translated protein, thereby regulating many cellular processes. Regulation miRNAs their function insulin resistance largely unexplored. The purpose this study was identify effects on contents with potential functions We examined miRs ‐1, ‐16, ‐23, ‐27, ‐133a, ‐133b,...
Obesity is a known risk factor for the development of hepatic disease; obesity-induced fatty liver can lead to inflammation, steatosis, and cirrhosis associated with degeneration mitochondria. Lifestyle interventions such as physical activity may ameliorate this condition. The purpose study was investigate regulation mitochondrial autophagy quality control in following Western diet-induced obesity voluntary activity. Eight-week-old C57BL/6J mice were fed diet (WD) or normal chow (NC,...
The accurate analytical characterization of metastatic phenotype at primary tumor diagnosis and its evolution with time are critical for controlling progression cancer.Here, we report a label-free optical strategy using Raman spectroscopy machine learning to identify distinct phenotypes observed in tumors formed by isogenic murine breast cancer cell lines progressively increasing propensities.Methods: We employed the 4T1 panel cells grow varying potential acquired spectra fiber probe-based...