A5037573153- Surfactants and Colloidal Systems
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Advanced Polymer Synthesis and Characterization
- Supramolecular Self-Assembly in Materials
- Environmental Chemistry and Analysis
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Dendrimers and Hyperbranched Polymers
- Bone Tissue Engineering Materials
- Drug Transport and Resistance Mechanisms
- Advancements in Transdermal Drug Delivery
- ATP Synthase and ATPases Research
- Antimicrobial agents and applications
- Chemical Synthesis and Reactions
- Advanced Drug Delivery Systems
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- Supramolecular Chemistry and Complexes
- Nanoparticle-Based Drug Delivery
- Electrostatics and Colloid Interactions
- Chemical Synthesis and Analysis
- Virus-based gene therapy research
Rede de Química e Tecnologia
2020-2024
Universidade do Porto
2011-2024
University of Minho
2017
Lund University
2008
Gene delivery targeting mitochondria has the potential to transform therapeutic landscape of mitochondrial genetic diseases. Taking advantage nonuniversal code used by mitochondria, a plasmid DNA construct able be specifically expressed in these organelles was designed including codon, which codes for an amino acid only if read ribosomes. In present work, gemini surfactants were shown successfully deliver mitochondria. Gemini surfactant-based complexes taken up cells through variety routes,...
The use of amino acids for the synthesis novel surfactants with vesicle-forming properties potentially enhances biocompatibility levels needed a viable alternative to conventional lipid vesicles. In this work, formation and characterization catanionic vesicles by newly synthesized lysine- serine-derived have been investigated means phase behavior mapping PFG-NMR diffusometry cryo-TEM methods. lysine-derived are double-chained anionic molecules bearing pseudogemini configuration, whereas...
Abstract Two series of novel cationic gemini surfactants based on the amino acid serine have been synthesized. These compounds contain long alkyl chains linked to nitrogen atoms residues, as well spacers interconnecting two carboxylic groups, through amide or ester bonds. The most efficient synthetic pathway was established; it involves introduction into N , ‐dialkylated monomeric precursors by peptide condensation methods with diamines diols, subsequent methylation and deprotection yield...
Cationic serine-based gemini surfactants have strong potential as compaction agents of nucleic acids for efficient non-viral gene delivery.
Abstract The synthesis of novel cationic gemini surfactants based on serine, with long lipophilic alkyl chains and a spacer linked to the nitrogen atom amino acid residue by amine linkages, is described. most efficient synthetic pathway involves introduction into monomeric precursors, N ‐alkyl derivatives, reductive amination dialdehydes followed methylation deprotection. Characterization basic micellization properties new compounds was carried out tensiometry conductimetry. These present...
Abstract Vesicles based on mixed cationic and anionic surfactants (catanionic vesicles) offer a number of advantageous colloidal features over conventional lipid‐based vesicles, namely spontaneity in formation, long‐term stability, easy modulation size charge. If biocompatibility is added through rational design the chemical components, potential for biorelated applications further emerges. Here, we report first time two catanionic vesicle systems which both ionic amphiphiles are derivatized...
The construction of multilayered films with tunable properties could offer new routes to produce biomaterials as a platform for 3D cell cultivation. In this study, produced five bilayers chitosan and alginate (CHT/ALG) were built using water-soluble modified mesyl tosyl⁻CHT via layer-by-layer (LbL) self-assembly. NMR results demonstrated the presences (2.83 ppm) tosyl groups (2.39, 7.37 7.70 in chemical structure chitosans. buildup was monitored by quartz-crystal-microbalance (QCM-D) film...
A greener and efficient one-pot methodology for the assembly of Glypromate® its structurally-related analogues by tandem sequential peptide coupling.
Cationic gemini surfactants have emerged as potential gene delivery agents they can co-assemble with DNA due to a strong electrostatic association. Commonly, complexation is enhanced by the inclusion of helper lipid (HL), which also plays key role in transfection efficiency. The formation lipoplexes, used non-viral vectors for transfection, through and hydrophobic interactions affected various physicochemical parameters, such cationic surfactant:HL molar ratio, (+/−) charge morphological...
Mixtures of cationic and anionic surfactants often originate bilayer structures, such as vesicles lamellar liquid crystals, that can be explored model membranes for fundamental studies or drug gene nanocarriers. Here, we investigated the aggregation properties two catanionic mixtures containing biomimetic derived from serine. The are designated 12Ser/8-8Ser 14Ser/10-10Ser, where mSer is a cationic, single-chained surfactant n-nSer an anionic, double-chained one (m n being C atoms in alkyl...