A5055396623- DNA Repair Mechanisms
- Nanocluster Synthesis and Applications
- Graphene and Nanomaterials Applications
- Carbon and Quantum Dots Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- PARP inhibition in cancer therapy
- Metal complexes synthesis and properties
- Cell death mechanisms and regulation
- Advanced Nanomaterials in Catalysis
- Radiation Therapy and Dosimetry
- Photodynamic Therapy Research Studies
- RNA modifications and cancer
- Ferrocene Chemistry and Applications
- Cancer-related molecular mechanisms research
- Petroleum Processing and Analysis
- RNA Research and Splicing
- Cancer therapeutics and mechanisms
- Phytochemicals and Antioxidant Activities
- Polyoxometalates: Synthesis and Applications
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Medical and Biological Ozone Research
- Melamine detection and toxicity
- Wound Healing and Treatments
- Effects of Radiation Exposure
University of Belgrade
2010-2025
Vinča Institute of Nuclear Sciences
2009-2023
Institute of Economic Sciences
2009-2010
The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed examine influence in vitro simulated presence complex food matrix antioxidant cytotoxic activity red wine. obtained results showed that total phenolic content sample after was higher compared undigested wine, while these samples similar before digestion. Furthermore, it has been noticed digested SKBR3 breast...
Nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity of new bimetallic Pt(<sc>ii</sc>) Pd(<sc>ii</sc>) complexes.
In this study, we investigated the cytotoxic effect of highly soluble dextran-coated CeO2 nanoparticles on human fetal lung fibroblasts MRC-5. We examined individual nanoparticle-treated cells by Raman spectroscopy and analyzed spectra using non-negative principal component analysis k-means clustering. way, determined dose-dependent differences between treated cells, which were reflected through intensity change lipid, phospholipid RNA-related modes. Performing standard biological tests for...
Apart from cytotoxicity, inhibitors of the COX-2 enzyme have demonstrated additional effects important for cancer treatment (such as radiosensitization tumor cells and cell antimigratory effects); however, relationship between inhibition other inflammation-related 5-LOX anticancer activity is still not well understood. In our study, cytotoxicity thirteen previously presented by group (1–13) was tested on three lines (HCT 116, HT-29 BxPC-3) one healthy line (MRC-5). Compounds 3, 5, 6 7 showed...
Quercetin, a well-known flavonoid with significant medicinal potential, was derivatized at the C8 position tetrahydroisoquinoline (THIQ) moiety, and physicochemical pharmacological properties, inhibition antioxidant activity, cytotoxicity of new compounds were evaluated. Physicochemical including lipophilicity, membrane permeability, P-glycoprotein substrate affinity, assessed theoretically using SwissADME software. The metal-chelating ability evaluated on metal ions Fe
ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position intensity fluorescent signal in each single cell. In practice there are problems quantification γH2AX. paper is based on two issues: determination...
Abstract Background The majority of CAKUT-associated CNVs overlap at least one miRNA gene, thus affecting the cellular levels corresponding miRNA. We aimed to investigate potency restitution CNV-affected remediate dysregulated expression target genes involved in kidney physiology and development vitro. Methods Heterozygous MIR484 knockout HEK293 homozygous MIR185 cell lines were used as models depicting deletion frequently affected by CNVs. After treatment with mimics, have been compared...
The literature data emphasize that nanoparticles might improve the beneficial effects of near-infrared light (NIR) on wound healing. This study investigates mechanisms synergistic healing potential NIR and silver metal–organic frameworks combined with nitrogen- sulfur-doped carbon dots (AgMOFsN-CDs AgMOFsS-CDs, respectively), which was conducted by testing fibroblasts viability, scratch assays, biochemical analysis, synchrotron-based Fourier transform infrared (SR-FTIR) cell spectroscopy...
Conventional radiotherapy with X- and gamma-rays is one of the common effective treatments cancer. High energy hadrons, i.e., charged particles like protons (12)C ions, due to their specific physics radiobiological advantages are increasingly used. In this study, effectiveness different radiation types evaluated on radio-resistant human HTB140 melanoma cells. The cells were irradiated gamma-rays, 62 MeV at Bragg peak in middle spread-out (SOBP), as well MeV/u ions. doses ranged from 2 24 Gy....
Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional for human CAKUT research. The study aimed to identify and functionally interpret frequently affected by estimate overall burden of on miRNA genes additional aim this was experimentally confirm effect a CNV candidate miRNA's expression subsequent change mRNA levels selected target genes. A database CAKUT-associated regions, created...
The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach induce cell apoptosis. This study was conducted evaluate the ability carbon ions ( [12] C) apoptosis and cycle arrest in human HTB140 melanoma cells.In this vitro study, were irradiated 62 MeV/n ion beam, having two different linear energy transfer (LET) values: 197 382 keV/μm. dose range 2 16 Gy. Cell viability estimated by sulforhodamine B assay seven days after...
Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of combined use these agents been analysed. Cells were irradiated in middle therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses 12 16 Gy and are those frequently used therapy. Four days after irradiation treated with fotemustine (FM) dacarbazine (DTIC). Drug concentrations 100 250 μM, values close produce 50% growth inhibition. Cell viability,...
<i>Background:</i> Metastatic melanoma is one of the most aggressive tumours and also very resistant to current therapeutic approaches. The aim this investigation was in vitro study anti-proliferative effects fotemustine (FM; 100 250 µ<i>M</i>), bevacizumab (5 µg/ml) proton irradiation (12 16 Gy) on HTB140 human cells. <i>Methods:</i> Viability estimated by sulphorhodamine B assay, while cell proliferation analyzed 5-bromo-2-deoxyuridine assay. Cell cycle...
This study aims to explore in vitro anti-tumor action of the equimolar mixture Fe(III)-substituted monolacunary Wells-Dawson POM (Fe-WD), K7[FeIII(α2-P2W17O61)(H2O)] and L(+)-ascorbic acid sodium salt (ascorbate) using cervical carcinoma HeLa cells as a model system. Exponentially growing were exposed Fe-WD ascorbate separately (0.005 – 0.5 mM) Fe-WD/ascorbate mixtures same concentrations for 48 72 h, at 37 °C. Single simultaneous exposure studied compounds resulted concentration-...
The cytotoxic effect of the mixture Fe(III)-substituted monolacunary Wells-Dawson polyoxometalate (Fe-WD), K7[FeIII(α2-P2W17O61)(H2O)] and L(+)-ascorbic acid sodium salt (ascorbate) on cervical carcinoma HeLa cells was evaluated in this study. Exponentially growing were treated vitro with Fe-WD (0.001 – 1 mM), forty times higher concentrations ascorbate (0.040 40 Fe-WD/ascorbate mixtures (concentration ratio : 40) for 24 h, at 37 °C. inhibited cell viability a concentration-dependent manner...
This study was conducted in order to evaluate the ability of carbon ions induce DNA double-strand breaks and apoptosis radio-resistant human HTB140 melanoma cells. The cells were irradiated with 12C having linear energy transfer 258 keV/mm. Irradiations performed dose range from 2 16 Gy. Induction evaluated hour after irradiation through expression gH2AX protein. Increased level detected samples especially high 12 Dose dependent increase 48 by flow-cytometry, maximum value 20.4% Gy,...
Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of epidermal growth factor receptor in cell proliferation and survival, there are indications that agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance antitumor by radiation. The aim this study to analyze inactivation effects g-rays test radiosensitizing potential erlotinib on human lung adenocarcinoma cells vitro. Irradiations were performed with doses ranging from 1 Gy 8 Gy....