A5069879386- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Connective tissue disorders research
- Proteoglycans and glycosaminoglycans research
- Spine and Intervertebral Disc Pathology
- Neonatal Respiratory Health Research
- Musculoskeletal pain and rehabilitation
- Immune responses and vaccinations
- Knee injuries and reconstruction techniques
- High Altitude and Hypoxia
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Fibroblast Growth Factor Research
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Periodontal Regeneration and Treatments
- Birth, Development, and Health
- Medical Imaging and Analysis
- dental development and anomalies
Southern University of Science and Technology
2020-2024
Wenzhou Medical University
2024
St Vincent's Hospital
2020
University of Michigan
2015-2019
Sun Yat-sen University
2009-2012
Accumulating evidence has implicated the deregluation of miRNAs in tumorigenesis. Previous studies have reported that microRNA-195 (miR-195) is markedly down-regulated human glioblastoma cells, compared with normal brain tissue, but biological role miR-195 development currently unknown. In this study, we define a tumor-suppressor for cells. Over-expression cell lines robustly arrested cycle progression and significantly repressed cellular invasion. We identified E2F3 CCND3 as functional...
Abstract Osteoblasts, which are the bone-forming cells, operate in a hypoxic environment. The transcription factors hypoxia-inducible factor-1α (HIF1) and HIF2 key mediators of cellular response to hypoxia. Both expressed osteoblasts. HIF1 is known be positive regulator bone formation. Conversely, role control osteoblast biology still poorly understood. In this study, we used mouse genetics demonstrate that an inhibitor osteoblastogenesis mass accrual. Moreover, provided evidence impairs...
The key focal adhesion protein β1 integrin plays an essential role in early skeletal development. However, roles of expression osteocytes during the regulation bone homeostasis and mechanotransduction are incompletely understood. To study vivo function osteocyte bone, we utilized 10-kb Dmp1 (Dentin matrix acidic phosphoprotein 1)-Cre to generate mice with deletion this cell type. Micro-computerized tomography, histomorphometry immunohistochemistry were performed determine effects loss on...
The role of osteocytes in maintaining bone mass has been progressively emphasized. Pip5k1c is the most critical isoform among PIP5KIs, which can regulate cytoskeleton, biomembrane, and Ca2+ release cells participate many processes, such as cell adhesion, differentiation, apoptosis. However, its expression function are still unclear. To determine osteocytes, was deleted by breeding 10-kb mouse Dmp1-Cre transgenic mice with Pip5k1cfl/fl mice. Bone histomorphometry, micro-computerized...
Genetically modified mice are the most useful tools for investigating gene functions in articular cartilage biology and pathogenesis of osteoarthritis. The AggrecanCreERT2 one reported mouse lines used this purpose. Prg4 (proteoglycan 4) encodes lubricin protein is expressed selectively chondrocytes located at superficial layer cartilage. While Prg4GFPCreERT2 knock-in inducible-Cre transgenic were generated a while ago, so far, few studies have line to perform functional biology.We recently...
Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) is a lipid kinase that plays pivotal role in the regulation of receptor-mediated calcium signaling multiple tissues; however, its skeleton not clear. Here, we show while deleting Pip5k1c expression mesenchymal stem cells using Prx1-Cre transgenic mice does impair intramembranous and endochondral ossification during skeletal development, it cause osteopenia adult mice, but rapidly growing young mice. We found loss dramatically...
Protein-encoding genes only constitute less than 2% of total human genomic sequences, and 98% genetic information was previously referred to as "junk DNA". Meanwhile, non-coding RNAs (ncRNAs) consist approximately 60% the transcriptional output cells. Thousands ncRNAs have been identified in recent decades, their essential roles regulation gene expression diverse cellular pathways associated with fundamental cell processes, including proliferation, differentiation, apoptosis, metabolism,...
ABSTRACT Hox genes are critical regulators of skeletal development and Hox9-13 paralogs, specifically, necessary for appendicular along the proximal to distal axis. Loss function both Hoxa11 Hoxd11 results in severe malformation forelimb zeugopod. In radius ulna these mutants, chondrocyte is perturbed, growth plates not established, maturation fails. compound mutants which one four Hox11 alleles remains wild-type, establishment a plate preserved embryos develop normally through newborn...
Emerging evidence implicates ER stress caused by unfolded mutant proteins in chondrocytes as the underlying pathology of chondrodysplasias. is triggered hypertrophic (HCs) a mouse model (13del) metaphyseal chondrodysplasia type Schmid (MCDS) misfolded collagen X proteins, but HCs do not undergo apoptosis; rather chondrocyte differentiation altered, causing skeletal abnormality. How 13del can escape from apoptosis and survive understood. Here we compared proteomes isolated growth plates with...
Intervertebral disc degeneration (IVDD) is a major cause of low back pain (LBP), worsened by chronic inflammatory processes associated with aging. Tumor necrosis factor alpha (Tnf-α) and its receptors, Tnf receptor type 1 (Tnfr1) 2 (Tnfr2), are upregulated in IVDD. However, pathologic mechanisms remain poorly defined.
Abstract Objective While joint immobilization is a useful repair method for intra-articular ligament injury and periarticular fracture, prolonged can cause multiple complications. A better understanding how remobilization impact function homeostasis will help clinicians develop novel strategies to reduce Design We first determined the effects of long-term on pain osteophyte formation in patients after an extraarticular fracture or injury. then developed mouse model harvested knee samples at...