A5009964656- RNA Research and Splicing
- RNA regulation and disease
- RNA modifications and cancer
- Renin-Angiotensin System Studies
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Plant biochemistry and biosynthesis
- Hedgehog Signaling Pathway Studies
- Insect Utilization and Effects
- Gastrointestinal motility and disorders
- Amino Acid Enzymes and Metabolism
- Diet and metabolism studies
- Obesity, Physical Activity, Diet
- Stress Responses and Cortisol
- Eating Disorders and Behaviors
- RNA and protein synthesis mechanisms
- Regulation of Appetite and Obesity
- Nitrogen and Sulfur Effects on Brassica
- Nitric Oxide and Endothelin Effects
- Plant Stress Responses and Tolerance
- Cancer-related gene regulation
- Immune Response and Inflammation
- Photosynthetic Processes and Mechanisms
University of Iowa
2007-2019
Vanderbilt University
2007
Vanderbilt University Medical Center
1998
Myocardial Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibition improves cardiac function following myocardial infarction (MI), but the CaMKII-dependent pathways that participate in stress responses are incompletely understood. To address this issue, we sought to determine transcriptional consequences of CaMKII after MI. We performed gene expression profiling mouse hearts with cardiomyocyte-delimited transgenic either a inhibitory peptide (AC3-I) or scrambled control (AC3-C) Of...
ADAR2 is a double-stranded-RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by site-selective conversion to inosine. Previous studies from our laboratory have demonstrated that can modify its own pre-mRNA create proximal 3' splice site containing noncanonical adenosine-inosine dinucleotide. Alternative splicing this acceptor adds 47 nucleotides mature transcript, thereby resulting loss functional protein expression due premature translation termination an alternate...
ADAR2 is a double-stranded RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by site-specific conversion to inosine. To examine physiologic consequences resulting from misexpression, we have generated mutant mice expressing either wild-type or deaminase-deficient transgenes under control human cytomegalovirus promoter. Transgenic inactive isoforms demonstrated adult onset obesity characterized hyperglycemia, hyperleptinemia, and increased adiposity. Paired feeding...
The present study tested the hypotheses that 1) nitric oxide (NO) is involved in attenuated responses to ANG II female mice, and 2) there differential expression of neuronal NO synthase (nNOS) subfornical organ (SFO) paraventricular nucleus (PVN) response systemic infusions males vs. females. Aortic blood pressure (BP) was measured conscious mice with telemetry implants. N(G)-nitro-l-arginine methyl ester (l-NAME; 100 microg x kg(.-1)day(-1)), an inhibitor NOS, administrated into lateral...
RNA editing is an alteration in the primary nucleotide sequences resulting from a chemical change base. observed eukaryotic mRNA, transfer RNA, ribosomal and non-coding RNAs (ncRNA). The most common mammalian central nervous system base modification, where adenosine residue base-modified to inosine (A I). Studies ADAR (adenosine deaminase that act on RNA) mutants Caenorhabditis elegans, Drosophila, mice clearly show process absolute requirement for homeostasis normal physiology of animal....
In humans, dysfunctional primary cilia result in Bardet-Biedl syndrome (BBS), which presents with clinical features including intellectual disabilities, obesity, and retinal degeneration, and, mouse models, the added feature of hydrocephalus. We observed increased Glial Fibrillary Acidic Protein (GFAP) immunoreactivity BBS brains. Increased GFAP expression is a hallmark astrocyte reactivity that associated microglia activation neuro-inflammation. To gain better understanding reactive...
Modulation of serotonin signaling by RNA editing the 2C receptor (5HT(2C) R) may be relevant to affective disorder as functions regulate mood and behavior. Previously, we observed enhanced endogenous behavioral despair in ADAR2 transgenic mice. As transcript 5HT(2C) R is a substrate ADAR2, hypothesized that perturbed equilibrium prefrontal cortex mice alters normal distribution edited amino acid isoforms modifies function downstream basal extracellular signal-regulated kinase (ERK)...
ADAR2 transgenic mice misexpressing the RNA editing enzyme (Adenosine Deaminase that act on RNA) show characteristics of overeating and experience adult onset obesity. Behavioral patterns brain changes related to a possible addictive in these were explored as display chronic hyperphagia. assessed their food preference motivation overeat competing reward environment with ad lib access running wheel food. Metabolic activity peripheral tissue [(18) F] fluorodeoxyglucose positron emission...
Previous studies from our laboratory have shown that there are sex differences in ANG II‐induced hypertension. ICV infusion of L‐NAME augments the pressor effects long‐term infusions II female mice but not males. However, mechanisms underlying these hypertension well understood. The present study tested hypothesis is differential expression nNOS response to systemic male vs and this may contribute We used both intact gonadectomized were either treated with (800 ng/kg/min; sc) or vehicle....