A5078522478- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Antibiotic Resistance in Bacteria
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Crystallography and molecular interactions
- Bacterial Identification and Susceptibility Testing
- Crystal structures of chemical compounds
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Acute Kidney Injury Research
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Infections and bacterial resistance
- Enterobacteriaceae and Cronobacter Research
- Vibrio bacteria research studies
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Vanadium and Halogenation Chemistry
- Parkinson's Disease Mechanisms and Treatments
- Escherichia coli research studies
- Neurological disorders and treatments
- Psoriasis: Treatment and Pathogenesis
Tongji University
2021-2025
Shanghai Pulmonary Hospital
2021-2025
Fudan University
2011-2025
Zhejiang Chinese Medical University
2024-2025
East China Normal University
2024
Nanjing University of Chinese Medicine
2016-2024
Anhui University of Science and Technology
2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
Minzu University of China
2024
Huaiyin Normal University
2021-2023
The rapid increase in carbapenem resistance among gram-negative bacteria has renewed focus on the importance of polymyxin antibiotics (colistin or E). However, recent emergence plasmid-mediated colistin determinants (mcr-1, -2, -3, -4, -5, -6, and -7), especially mcr-1, carbapenem-resistant Enterobacteriaceae is a serious threat to global health. Here, we characterized novel mobile gene, mcr-8, located transferrable 95,983-bp IncFII-type plasmid Klebsiella pneumoniae. deduced amino-acid...
K1/K2 hvKP strains acquire carbapenem-resistance plasmids, known as CR-hvKp, and carbapenem-resistant Klebsiella pneumoniae (CRKP) obtain virulence recognized hv-CRKP. The two different evolution patterns of hypervirulent combined may lead to their prevalence in hospitals. Our study aimed investigate the hv-CRKP CR-hvKp analyze factors influencing prevalence. We collected 890 K. genomes from GenBank 530 clinical isolates nine found that were more prevalent than both dominated by blaKPC-2...
Inflammation plays an important role in the initiation and progression of acute kidney injury (AKI). However, evidence regarding prognostic effect platelet-to-lymphocyte ratio (PLR), a novel systemic inflammation marker, among patients with AKI is scarce. In this study, we investigated value PLR predicting outcomes critically ill AKI. Patient data were extracted from Multiparameter Intelligent Monitoring Intensive Care Database III version 1.3. cutoff values determined using smooth curve...
Recently, a novel mobile colistin resistance gene, mcr-8, was identified in Klebsiella pneumoniae. Here, we report the identification of mcr-8 and its variant, mcr-8.4, Raoultella ornithinolytica isolates which also belong to Enterobacteriaceae family. The gene located on transferrable plasmids with difference sizes. Notably, transferability mcr-8-carrying enhanced once they entered into Escherichia coli hosts multiple β-lactamase genes could co-transfer mcr-8. These findings expand our...
Mobile plasmids play a key role in spurring the global dissemination of multidrug-resistant (MDR) K. pneumoniae, while plasmid curing has been recognized as promising strategy to combat antimicrobial resistance. Here we exploited pneumoniae native CRISPR system cure high-risk IncFII plasmids.We examined matched protospacers 725 completely sequenced from genomes. Then, re-engineered CRISPR-Cas3 and deliver via conjugation. Plasmid killing efficiency G. mellonella infection model were applied...
To cite this article: Liu X, Wang G, Hong D, Tsai H‐J, Zhang S, Arguelles L, Kumar R, H, Zhou Y, Pearson C, Ortiz K, Schleimer Holt P, Pongracic J, Price HE, Langman X. Gene–vitamin D interactions on food sensitization: a prospective birth cohort study. Allergy 2011; 66 : 1442–1448. Abstract Background: It has been hypothesized that vitamin deficiency (VDD) contributes to the development of sensitization (FS) and then allergy. However, epidemiological evidence is conflicting. We aim examine...
Significant challenges are present in antibiotic drug discovery and development. One of these is the number efficient approaches Gram-negative bacteria have developed to avoid intracellular accumulation drugs other cell-toxic species. In order better understand processes correlate vitro enzyme inhibition whole cell activity, a assay evaluate key factor, drug, urgently needed. Here, we describe unique liquid chromatography (LC)-mass spectrometry (MS) approach measure amount cellular uptake...
Abstract Bromodomain containing 7 (BRD7) was identified as a nuclear transcriptional regulatory factor. BRD7 functions tumor suppressor in multiple cancers, including nasopharyngeal carcinoma (NPC). In this study, we reported novel mechanism of NPC progression. We demonstrated that the expression miR-141 remarkably increased tissues and negatively correlated with survival rate patients. Decreased levels miR-141, primary, precursor mature forms were found BRD7-overexpressing HEK293, 5-8F HNE1...
Abstract Background We aimed to investigate neuromelanin‐sensitive magnetic resonance imaging (NM‐MRI) features in the locus coeruleus of de novo Parkinson's disease patients with different cognitive states and determine whether these are associated impairment. Methods Three groups subjects were recruited this study, including PD mild impairment (n = 23), without 48), control 32). All underwent clinical evaluations, as well MRI scanning. The contrast‐to‐noise ratio images cortical thickness...
Virulence plasmids of hypervirulent Klebsiella pneumoniae (hvKp) have the potential to transfer drug-resistant strains or integrate with other plasmids, facilitating genome evolution threatening pathogens. We conducted an in-depth analysis publicly available 156 complete sequences hvKp together a multi-region clinical cohort 171 from China provide evidence for virulence plasmid evolution. were frequently detected in ST23 and ST11 K. strains. Multidrug-resistant (MDR-hvKp) occupied large...
Klebsiella pneumoniae is one of the most common Gram-negative bacilli isolated from bloodstream infections worldwide, and recently an increased rate carbapenem resistance has been reported in this pathogen. This study aims to describe genomic characteristics carbapenem-resistant K. (CRKP) patients with bacteremia China. We analyzed 147 isolates attended 12 referral hospitals China between April 2015 November 2018. conducted a phenotypic susceptibility evaluation whole genome sequence...
The synthesis and characterization of four complexes containing naturally occurring binding groups are reported: VO(pic)2·H2O (Hpic = picolinic or pyridine-2-carboxylic acid), NH4[VO2(pic)2]·2H2O, VO(oz)2 (Hoz 2-(2'-hydroxyphenyl)-2-oxazoline), VO(thoz)2 (Hthoz 2-(2'-hydroxyphenyl)-2-thiazoline). X-ray structures VO(oz)2, have been determined. Crystals NH4[VO2(pic)2]·2H2O (C12H12N3O6V·2H2O) monoclinic, space group Cc, a 10.347(2) Å, b 26.318(2) c 7.247(1) β 128.37(1)°, V 1547.3(5) Å3, Z 4;...
Abstract Background Animal and human studies suggest that inflammation malnutrition are common in acute kidney injury (AKI) patients. However, only a few reported CRP, marker of inflammation, albumin, prealbumin cholesterol, markers nutritional status were associated with the prognosis AKI No study examined whether combination inflammatory could predict mortality Methods 155 patients hospital-acquired recruited to this prospective cohort according RIFLE (Risk, Injury, Failure, Lost or End...
We retrospectively studied a random cohort of patients with cerebral trauma to investigate the risk factors acute kidney injury (AKI) following trauma. AKI was determined using RIFLE (risk, injury, failure, loss, or end-stage kidney) staging criteria. About 171 were chosen in study, 53 group and 118 without non-AKI group. By logistic regression analysis, univariate analysis revealed that age, hypertension, emergent surgery, systemic inflammatory response syndrome (SIRS), Glasgow coma score...
Differentiating early-stage Parkinson's disease (PD) from essential tremor (ET) remains challenging. In the current study, we aimed to evaluate whether visual analyses of neuromelanin-sensitive magnetic resonance imaging (NM-MRI) combined with nigrosome-1 (N1) using quantitative susceptibility mapping (QSM) in substantia nigra (SN) are diagnostic value differentiation de novo PD untreated ET. Sixty-eight patients PD, 25 ET, and 34 control participants underwent NM-MRI QSM. NM N1 signals SN...
Abstract Background The pandemics caused by MDR Klebsiella pneumoniae are mostly due to the global dissemination of high-risk clonal complex 258 (CC258) and related IncF epidemic plasmids. However, factors leading epidemiological advantages CC258–IncF linkage remain obscure. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) CRISPR-associated protein (CRISPR-Cas) systems, providing adaptive immunity against invading DNA, play an important role in interactions between plasmids...
Klebsiella pneumoniae carbapenemase (KPC)-producing K. (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates blaKPC-IncF plasmids. CRISPR-Cas system an adaptive immune that can hinder gene expansion driven by horizontal transfer. Because plasmids are favored pneumoniae, it was interest examine co-distribution CRISPR in such isolates. We collected 459...
In recent years, the hypervirulent and carbapenem-resistant Klebsiella pneumoniae has been increasingly reported worldwide. The objective of this study was to compare antibiotic resistance virulence profiles K.pneumoniae (CR-hvKP) (hv-CRKP) identify prevailing strain in clinical settings.
ABSTRACT Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory tract infections in young children and other high-risk populations. RSV nucleoprotein (N) essential for assembly replication as part viral ribonucleoprotein (RNP) complex. RSV604 was a putative N inhibitor phase 2 clinical trials whose molecular mechanism action (MoA) not well understood. This study investigated cell line-dependent potency demonstrated its direct binding to protein vitro , providing...
The emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) has become a hot topic confounding problem for clinicians researchers alike. Conjugative virulence plasmids have the potential to cause more threatening dissemination hv-CRKP strains. We previously identified K2606, CG23 clinical strain harboring conjugative plasmid designated pK2606. In this study we examined hypervirulence levels using assays biofilm formation, serum resistance, wax larvae mouse in vivo...
The rapid emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and the comparatively limited development new antibiotics pose a major threat to public health. Aminoglycosides are important options that can lower mortality rate effectively in combination therapy with β-lactam agents. However, this study, we observed two multidrug-resistant (MDR) K. named 1632 1864 exhibited high-level resistance both carbapenems aminoglycosides. Through whole-genome sequencing (WGS), unusual...