A5059680275- Antibiotic Resistance in Bacteria
- Lymphoma Diagnosis and Treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Viral-associated cancers and disorders
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Antibiotics Pharmacokinetics and Efficacy
- CNS Lymphoma Diagnosis and Treatment
- Vibrio bacteria research studies
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Drug Transport and Resistance Mechanisms
- Chronic Lymphocytic Leukemia Research
- HER2/EGFR in Cancer Research
- Neuroendocrine Tumor Research Advances
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Advanced Breast Cancer Therapies
- Glycosylation and Glycoproteins Research
Henan University
2025
Hebei University
2020-2024
University of Chinese Academy of Sciences
2020-2024
Chinese Academy of Sciences
2020-2024
Zhejiang Cancer Hospital
2013-2024
The University of Texas MD Anderson Cancer Center
2022-2024
China Agricultural University
2015-2021
Cancer Hospital of Chinese Academy of Medical Sciences
2021
Zhejiang Chinese Medical University
2019
Freie Universität Berlin
2019
The mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one last-resort antibiotics for treatment severe clinical infections caused by multidrug-resistant Gram-negative bacteria. To date, six slightly different variants mcr-1, and a second gene, mcr-2, have been reported or annotated in GenBank database. Here, we characterized third mcr-3 coexisted with 18 additional determinants 261-kb IncHI2-type plasmid pWJ1 from porcine...
The rapid increase in carbapenem resistance among gram-negative bacteria has renewed focus on the importance of polymyxin antibiotics (colistin or E). However, recent emergence plasmid-mediated colistin determinants (mcr-1, -2, -3, -4, -5, -6, and -7), especially mcr-1, carbapenem-resistant Enterobacteriaceae is a serious threat to global health. Here, we characterized novel mobile gene, mcr-8, located transferrable 95,983-bp IncFII-type plasmid Klebsiella pneumoniae. deduced amino-acid...
The identification of the first mobile colistin resistance (MCR) gene, mcr-1, in 2015 triggered a rash mcr screening reports. Subsequently, nine MCR-family genes and their variants have been described. However, comprehensive overview concerning epidemiology whole MCR family, which is essential for facilitating rational interventions against dissemination, lacking. Here, based on National Database Antibiotic Resistant Organisms published studies, we summarized latest epidemiological...
Mitochondrial damage-associated molecular patterns (DAMPs) including mitochondrial DNA (mtDNA), TFAM (transcription factor A, mitochondrial), and ATP, which play crucial roles in the regulation of inflammatory environment human diseases. However, role DAMPs regulating tumor microenvironment (TME) remains unclear. Herein, we demonstrate that infiltration M2-type tumor-associated macrophages (TAMs) was correlated with resistance hepatocellular carcinoma (HCC) to sorafenib. We found cell-free...
Abstract Despite unprecedented responses of some cancers to immune checkpoint blockade (ICB) therapies, the application inhibitors in pancreatic cancer has been unsuccessful. Glucocorticoids and glucocorticoid receptor (GR) signaling are long thought suppress immunity by acting on cells. Here we demonstrate a previously undescribed tumor cell-intrinsic role for GR activating PD-L1 expression repressing major histocompatibility complex class I (MHC-I) ductal adenocarcinoma (PDAC) cells...
ABSTRACT Two adjacent colistin resistance gene variants, termed mcr-3.3 and mcr-3 -like, were identified in the chromosome of an Aeromonas veronii isolate obtained from retail chicken meat. The variants showed 95.20% 84.19% nucleotide sequence identity, respectively, to porcine Escherichia coli . Functional cloning indicated that only conferred polymyxin both E. salmonicida mcr-3.3-mcr-3 -like segment was also observed other species, including A. media , caviae hydrophila
ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) have spread worldwide, leaving very few treatment options available. New Delhi metallo-beta-lactamase (NDM) is the main carbapenemase mediating CRE resistance and of increasing concern. NDM-positive human origin are frequently identified; however, emergence NDM, particularly novel variants, in bacteria food animal has never been reported. Here, we characterize a NDM variant (assigned NDM-17) identified β-lactam-resistant sequence type 48...
The aim of the study was to develop a multiplex assay for rapid detection mcr-1, mcr-2, and mcr-3, group genes conferring resistance colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR has been designed detect mcr genes, applied cultured bacteria, feces soil samples. All three could be detected with lower limit 102 bacteria. This test highly specific sensitive, generated no false-positive results. also conclusive when samples containing mcr-1-positive...
MicroRNA is a type of endogenous non-coding RNA implicated in various cellular processes, and has been intensely investigated the field cancer research for many years. Here, we functions mechanisms miR-124 prostate cancer, which putative tumor suppressor reported carcinomas.Using bioinformatics, talin 1 was indicated as potential target miR-124. We examined expression levels tissue specimens cell lines. To explore relationship between 1, mimics, inhibitors, small interfering (siRNA) were...
Abstract Background Pancreatic cancer is one of the most lethal cancers worldwide. The IAPs function as E3 ubiquitin ligases and contribute to pancreatic initiation, progression, metastasis. Although IAP-targeted therapies have been developed shown anticancer efficacy in preclinical settings, none them has approved yet. Methods Transcriptome data from public datasets were used analyze correlation E2s, biological E2 UbcH5c cancer. A structure-based virtual screen was identify inhibitor,...
Single-molecule fluorescence in situ hybridization (smFISH) represents a promising approach for the quantitative analysis of nucleic acid biomarkers clinical tissue samples. However, low signal intensity and high background noise are complications that arise from diagnostic pathology when performed with smFISH-based RNA imaging formalin-fixed paraffin-embedded (FFPE) specimens. Moreover, associated complex procedures can produce uncertain results poor image quality. Herein, by combining...
Mobile colistin resistance enzyme MCR-3 is a phosphoethanolamine transferase modifying lipid A in Gram-negative bacteria. generally mediates low-level (≤8 mg L-1 ) among Enterobacteriaceae, but occasionally confers high-level (>128 aeromonads. Herein, it determined that MCR-3, together with another modification mediated by the arnBCADTEF operon, may be responsible for Lipid critical site of pathogens Toll-like receptor 4 recognizing. However, unknown whether or how MCR-3-mediated affects...
Paired box 6 (PAX6) plays a significant role in the development of human neuroectodermal epithelial tissues. Previous studies have suggested that PAX6 promoter is hypermethylated breast cancer and it involved cell proliferation. The present study aimed to investigate expression invasive tissues, evaluate its prognostic significance. Immunohistochemistry (IHC) was used detect on tissue microarray containing tissues from 111 patients. Associations with staging prognosis were analyzed. mainly...
Since the first report of plasmid-mediated, colistin-resistant gene, mcr-1, nine mcr genes and their subvariants have been identified. The spreading scope mcr-1~10 varies greatly, suggesting that may different evolutionary advantages. Depending on MCR family phylogeny, mcr-6 is highly similar to mcr-1 -2, mcr-7~10 are mcr-3 -4. We compared expression effects MCR-1~5 bacteria common physiological background. MCR-1-expressing strain showed better growth than did MCR-2~5-expressing strains in...
Metallo-β-lactamase gene blaVIM was identified on the chromosome of four Pseudomonas sp. isolates from a chicken farm, including one aeruginosa isolate swallow (Yanornis martini), putida fly, and two P. chickens. The shared variants blaVIM-carrying genomic contexts that resemble corresponding regions clinical metallo-β-lactamase-producing spp. Our study suggests surveillance carbapenemase-producing bacteria in livestock their surrounding environment is urgently needed.
The global emergence of plasmid-mediated colistin resistance genes mcr-1 and mcr-3 has threatened the role “last-resort” drug in defense against infections caused by multidrug-resistant Gram-negative bacteria. However, functional differences between these two mediating remain poorly understood.
In this study, we aimed to clarify the role of PIM-1 in papillary thyroid carcinoma (PTC) vitro and investigate relationship between redox proteins (NOX4, SOD2, GPX2) at tissue cellular levels. As a inhibitor, SGI-1776 inhibited cell proliferation, colony formation, migration induced an increase apoptosis reactive oxygen species two PTC lines (BCPAP TPC-1). The expressions PIM-1, SOD2 GPX2 were downregulated after siNOX4 exposure. Immunohistochemistry 120 patients showed that all four...