The aim of this study was to investigate the role microRNA-21 (miR-21) in regulation phosphatase and tensin homolog deleted from chromosome-10 (PTEN) expression proliferation endometrioid endometrial cancer (EEC) cells. We performed a qRT-PCR assay with miR-21 PTEN 16 paired EEC tumor tissues adjacent non-tumor endometrium. To by miR-21, we designed gain- loss-of-function experiments KLE cell line transfection synthetic mimic inhibitor. validate putative binding site 3' untranslated region...

Epithelial mesenchymal transition (EMT) is involved in the pathogenesis of adenomyosis, and Notch signaling crucial to EMT. The objective this study was explore Notch1/Numb/Snail adenomyosis.The expression levels members cascade normal endometria (proliferative phase: n = 15; secretory postmenopausal 15) adenomyotic were determined by immunohistochemistry analysis.We found that expressions Notch1 EMT-related proteins N-cadherin, Snail Slug upregulated ectopic endometrium adenomyosis compared...

// Zhao Liu 1 , Shasha Qi 2 Xingbo Mingjiang Li Sentai Ding Jiaju Lu and Hui Zhang Department of Urology, Shandong Provincial Hospital Affiliated to University, Jinan, Shandong, People's Republic China Obstetrics Gynecology, Correspondence to: Zhang, email: Keywords : metformin, βKlotho, 17β-estradiol, epithelial-mesenchymal transition, endometrial adenocarcinoma Received August 14, 2015 Accepted January 19, 2016 Published 27, Abstract The potential role metformin in treating cancer remains...

Melatonin is a potential therapeutic agent for endometriosis, but its molecular mechanism unclear. Here, we investigated the effect of melatonin on epithelial-mesenchymal transition (EMT) in endometriotic endometrial epithelial cells and explored pathway that might be involved.This hospital-based study included 60 women reproductive age using endometrium immunohistochemistry, 6 undergoing bilateral tubal ligation patients with endometriosis isolation or subsequent analysis, respectively. We...

Preeclampsia is an idiopathic and serious complication during gestation in which placental trophoblast cells differentiate into several functional subtypes, including highly invasive extravillous trophoblasts (EVTs). Although the cause pathogenesis of preeclampsia have remained unclear, numerous studies suggested that inadequacy EVT invasion leads to imperfect uterine spiral artery remodelling, plays a crucial role development preeclampsia. Rac1, or Ras-related C3 botulinum toxin substrate...

Endometrial cancer (EnC) is a malignant gynecological tumor commonly observed in developed countries, specifically among post‑menopausal women. Although numerous patients with EnC receive promising prognoses, those advanced or metastatic disease often have poor prognosis and an impaired quality of life. Tripartite motif‑containing 22 (TRIM22) has been confirmed to play many crucial roles different biological processes, from inflammatory tumorigenesis. However, the multifaceted TRIM22 remain...

Abstract This study aims to examine the expression of p53, p16, and murine double minute 2 (MDM2) protein in normal endometrium endometriosis, order discuss role MDM2 apoptosis pathogenesis development provide a theoretical basis for clinical diagnosis treatment. The immunohistochemical streptavidin-biotin peroxidase method was used detect tissue samples obtained from 30 women with pathologically confirmed ovarian endometriosis 29 endometrium. relationship between analyzed. In endometrium,...

This article was aimed to study the FOXF2 effects on cervical cancer. Tumor tissues and adjacent of 41 cancer patients were collected. Human endometrial epithelial cells (hEEC) Hela cultured. expression vector its empty transfected into cells, named as pcDNA 3.1-FOXF2 group Vector group, respectively. without any treatment set Blank group. qRT-PCR used detect mRNA expression. Nude mouse xenograft assay performed test proliferation ability in vivo β-catenin positive cell numbers detected by...

Background Endometrial adenocarcinoma (EAC) is one of the most commonly diagnosed gynaecological malignancies among female population developed countries. DUSP6 a negative regulator ERK signaling, which molecular switch involved in MAPK signaling during progress malignancies. was previously found to inhibit tumorigenesis and EMT-associated properties several cancers, however, its exact role EAC remains unclear Methods The level DUSP6, (E-cad) (N-cad) cancerous tissues respective adjacent...

Objective To investigate the adjuvant efficacy of metformin treatment to achieve pathological complete response (CR) in patients with endometrial complex hyperplasia (CH) and atypical (CAH), secondarily, evaluate their pregnancy outcomes after following assisted reproductive technology (ART). Study Design This prospective cohort study analyzed 219 diagnosed infertility CH/CAH from January 2016 December 2020. Among these patients, 138 were assigned control group (progesterone alone) 81...

Objective To investigate the expression and humoral immune response of sperm-associated antigen 9 (SPAG9) in endometri al carcinoma. Methods Sperm-associated gene levels were evaluated endometrial carcinoma, hyperplasia, adjacent tissues, normal tissues by reverse transcriptase-polymerase chain reaction, immunohistochemistry, Western blot. concentration serum samples from 10 healthy women, 20 women with benign diseases, 50 carcinoma was detected enzyme-linked immunosorbent assay. Results (1)...

Objectives: To explore the possible risk factors for cesarean scar pregnancy (CSP), incidence of which is increasing rapidly in China. Material and methods: 79 patients with CSP 69 non-CSP expectant mothers at least 1 previous section were employed study. The obstetric histories participants collected analyzed using Chi square test. Results: We found that 77.2% had ≥ 3 pregnancies only 36.2% women pregnacies group. During delivery, 21.5% entered first stage labor, was 43.5% group ( P <...

Background: Blood reperfusion after ischemia is the main measure to restore cell function. This study was aimed explore effect of propofol on rat and models liver ischemia-reperfusion (I/R) injury, investigate its possible mechanism. Methods: Wistar rats were divided into four groups: control group, sham I/R group. Human hepatocyte HL7702 six group (5, 10, 20 40 µmol/L) groups. After animal established, alanine aminotransferase (ALT), aspartate (AST), malondialdehyde (MDA) adenosine...

Objective. To explore the stroma–epithelium interactions in endometriosis and to identify possible signalling pathways involved this cross-talk.

Cervical cancer (CC) is one of the most common gynecological malignancies, and metastasis limits use surgical resection. Metapristone (MIF) was reported to suppress proliferation migration several cells. Exosomes play a variety roles in cellular biological processes. The relation exosomes CC less studied. Cell viability, apoptosis assay conducted HeLa cells treated by MIF CCK-8 kit, staining Annexin V-fluorescein isothiocyanate propidium iodide, wound test respectively. ISG15 expression...