Th17 cells have been associated with immune-mediated diseases in humans but it has still not determined whether they play a role immune thrombocytopenia. We evaluated representative cytokines of the Th17, Th1, Th2 and Treg cell commitment serum patients chronic thrombocytopenia, as well source IL-17A. Higher levels IL-17A Th17-related cytokines, an increased percentage producing CD4+ neutrophils were observed patients. The involved Th1 IFN-γ, IL-2, IL12-p70 percentages also increased, IL-4...

Myiasis, a condition stemming from the parasitic infestation of Diptera fly larvae, constitutes complication for cancer patients, particularly those with malignant skin wounds. The factors that contribute to myiasis include old age, inadequate hygiene, poor living conditions, vascular disease, and diabetes. Cases in neoplastic wounds head neck region are rare guidelines or recommendations regarding best treatment approach remain lacking. Herein, we describe case developed into an extensive...

Knowledge of Leishmania virulence is essential for understanding how the contact between pathogen and host cells can lead to pathogenesis. Virulence in two L. infantum strains was characterized using macrophages hamsters. Next, we used difference gel electrophoresis (DIGE) mass spectrometry identify differentially expressed proteins. A total 63 spots were identified corresponding 36 proteins; 20 up-regulated, which 16 had been previously associated with virulence. Considering our results...

Summary Canine visceral leishmaniasis ( CVL ) is a severe and fatal systemic chronic inflammatory disease. We investigated the alterations in, potential associations among, antioxidant enzymes, trace elements histopathology in . Blood tissue levels of Cu ‐ Zn superoxide dismutase, catalase glutathione peroxidase were measured mixed‐breed dogs naturally infected with L eishmania infantum chagasi , symptomatic n = 19) asymptomatic 11). Serum copper, iron, zinc, selenium nitric oxide, plasma...

Background American tegumentary leishmaniasis (ATL) is endemic in Latin America, where Brazil has over 27 thousand cases per year. The aim of the present study was to develop an immunohistochemical method (IHC) for ATL diagnosis. For this purpose, we used serum from a dog naturally infected with Leishmania (L) infantum (canine hyperimmune serum) as primary antibody, followed by detection system secondary biotinylated antibody. Methodology Skin samples were obtained 73 patients area...

Background Leishmania (Viannia) braziliensis has been associated with a broad range of clinical manifestations ranging from simple cutaneous ulcer to destructive mucosal lesions. Factors leading this diversity presentations are not clear, but parasite factors have lately recognized as important in determining disease progression. Given the fact that activity ecto-nucleotidases correlates parasitism and development infection, we evaluated these enzymes promastigotes 23 L. isolates possible...

Interferon gamma (IFN-γ) is a key factor in the protection of hosts against intracellular parasites. This cytokine induces parasite killing through nitric oxide and reactive oxygen species production by phagocytes. Surprisingly, during Leishmania amazonensis infection, IFN-γ plays controversial roles. During vitro infections, proliferation amastigote forms L. amazonensis. However, this not essential at beginning an vivo infection. It clear why does mediate early stages Thus, aim our study...

We describe the relationship between lesion outcome and histopathological hallmarks in susceptible (BALB/c) resistant (C57BL/6 IL-4-deficient BALB/c) mouse strains over course of a 12-week-infection with Leishmania major ear. The infiltration mononuclear cells polymorphonuclear occurred within 6 h predominated one week post-infection. Permissive intracellular growth pathogen was associated non-healing lesions. In contrast, tissue damage clearance parasite observed healing lesions inducible...

Methyl benzoate and methyl p-chlorophenyl acetate react with neat benzylamine pyrrolidine to form the corresponding amides. These reactions are faster in presence of 20 mol% DBU providing slight better yields. When a diester derived from L-aspartic acid was used as substrate, reaction chemoselective led amides good Reaction aspartic monomethyl ester these amines having free carboxy group (at C1). Less nucleophilic less basic aniline failed expected products both absence DBU. By monitoring...

In South America, visceral leishmaniasis is frequently caused by Leishmania infantum and, at an unknown frequency, amazonensis. Therefore, mixed infections with these organisms are possible. Mixed might affect the clinical course, immune response, diagnosis, treatment and epidemiology of disease. Here we describe course L. amazonensis in a hamster model. We show that associated more severe disease than infection or alone. spleens infections, outcompeted tissue, but not culture from tissue....

Several reports have shown that cutaneous leishmaniasis lesions are painless, suggesting Leishmania infection interferes with pain perception. Comparisons of inflammation-induced hyperalgesia between BALB/c and C57BL/6 mice been little explored in the literature, comparative data regarding nociception non-existent. In susceptible resistant were intradermally inoculated a low dose major ear, we investigated variation over 12-wk period post-infection this variation's association structure...

Summary Hepatic fibropoiesis has been confirmed in canine visceral leishmaniasis. In fibrotic disease, hepatic stellate cells ( HSC ) play an important role fibropoiesis, undergoing activation by TGF ‐β to acquire characteristics of myofibroblasts. These show extensive capacity for proliferation, motility, contractility, collagen synthesis and extracellular matrix component synthesis. The aim this work was identify markers 10 symptomatic asymptomatic dogs naturally infected with Leishmania...

Methods for the development and validation determination of iron zinc in human serum hair samples by graphite furnace atomic absorption spectrometry (GF AAS) were performed.Solubilization was immediate manual agitation both with a 10 mL volume sample plus deionized water.Optimum pyrolysis atomization temperatures obtained temperature curves matrices.For samples, best 1400 2500 °C (Fe) 700 1600 (Zn), respectively.For 1000 2400 800 respectively.Permanent modifiers zirconium presented results...

A low cost and efficient method to determine Cd Pb levels in cassava by graphite furnace atomization atomic absorption spectrometry (GF AAS) employing slurry sampling was developed. Slurries were prepared cryogenic grinding of samples (40 mg), sieving for grain size at 50 mesh addition 2% v/v nitric acid 1% hydrogenous peroxide up 2 mL volume. The maintained homogeneous using air bubbling an aquarium pump. Tungsten (500 μg) titanium the best permanent modifiers Pb, respectively. parameters...

O modelo experimental canino Golden Retriever portador da Distrofia Muscular (GRMD) é o melhor substituto entre os modelos animais para estudar a de Duchenne. Além musculatura estriada, doença pode afetar estriada cardíaca e lisa, desta forma, funcionamento do trato digestório, já que músculo liso elemento primário dos órgãos tubulares. Através estudo morfológico descritivo, objetivo deste trabalho foi verificar se distrofia muscular afeta arquitetura geral digestório como dispõe sua...

Magnetic resonance imaging (MRI) is a well-used technique to assess muscle size and can be acquired on different planes. Pectoralis major (PM) triceps brachii (TB) muscles are often analyzed the axial plane, however it unknown if anatomical cross-sectional area (CSA) calculated from planes will affect size-strength relationship. Thus, first aim of present study was identify CSA PM TB measured presents similar A secondary investigate quantification between sagittal plane.Fifteen males...

In this work efficient methods to determine copper and iron in dog serum samples by graphite furnace atomic absorption spectrometry were developed. The diluted at a 1 : 9 or 19 (for Cu Fe, respectively) ratio with 1% (v/v) nitric acid containing 0.1% Triton X-100 solution. Rhodium for no modifier proved be the better permanent modifier. Optimization included fractional factorial planning using Pareto CCD designs. For both analytes working linear range was 0–100 mg L−1 (r2 > 0.99). obtained...

Despite the very low or absent parasitism in lungs, interstitial pneumonitis is a common lesion found humans and dogs with visceral leishmaniasis. The lung neglected organ study of leishmaniasis, but represents an important characterized by thickening alveolar septum due to fibrosis inflammatory exudate, its pathogenesis still uncertain. In this study, polymerase chain reaction (PCR) was used detect Leishmania infantum paraffin-embedded biopsies from naturally infected endemic area Minas...