A5022315172- Genomics and Chromatin Dynamics
- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- Hedgehog Signaling Pathway Studies
- Inflammatory Biomarkers in Disease Prognosis
- Insect and Arachnid Ecology and Behavior
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- BRCA gene mutations in cancer
- Peroxisome Proliferator-Activated Receptors
KU Leuven
2005-2023
VIB-KU Leuven Center for Brain & Disease Research
2019-2023
Institut du Cerveau
2018-2021
Sorbonne Université
2021
Beijing Luhe Hospital Affiliated to Capital Medical University
2020
VIB-KU Leuven Center for Cancer Biology
2008-2016
Vlaams Instituut voor Biotechnologie
2004-2009
Université Libre de Bruxelles
2000-2007
Single-cell technologies allow measuring chromatin accessibility and gene expression in each cell, but jointly utilizing both layers to map bona fide regulatory networks enhancers remains challenging. Here, we generate independent single-cell RNA-seq ATAC-seq atlases of the Drosophila eye-antennal disc spatially integrate data into a virtual latent space that mimics organization 2D tissue using ScoMAP (Single-Cell Omics Mapping spatial Axes Pseudotime ordering). To validate predicted...
A comprehensive systems-level understanding of developmental programs requires the mapping underlying gene regulatory networks. While significant progress has been made in a few such networks, almost all networks cell-fate specification remain unknown and their discovery is significantly hampered by paucity generalized, vivo validated tools target functional enhancer discovery. We combined genetic transcriptome perturbations computational analyses to identify large cohort genes proneural...
Wound response programs are often activated during neoplastic growth in tumors. In both wound repair and tumor growth, cells respond to acute stress balance the activation of multiple programs, including apoptosis, proliferation, cell migration. Central those responses JNK/MAPK JAK/STAT signaling pathways. Yet, what extent these cascades interact at cis-regulatory level how they orchestrate different regulatory phenotypic is still unclear. Here, we aim characterize states that emerge...
The neurogenin (Ngn) transcription factors control early neurogenesis and neurite outgrowth in mammalian cortex. In contrast to their proneural activity, function growth is poorly understood. Drosophila has a single predicted Ngn homolog, Tap, of unknown function. Here we show that Tap not protein but required for proper axonal guidance neurons the mushroom body, neuropile associative learning memory. Genetic expression analyses suggest inhibits excessive by fine regulation levels Wnt...
How conserved pathways are differentially regulated to produce diverse outcomes is a fundamental question of developmental and evolutionary biology. The process neural precursor cell (NPC) selection by basic helix-loop-helix (bHLH) proneural transcription factors in the peripheral nervous system (PNS) atonal related proteins (ARPs) presents an excellent model which address this issue. Proneural ARPs belong two highly groups: ATONAL (ATO) group NEUROGENIN (NGN) group. We used cross-species...
Neuronal specification is regulated by the activity of transcription factors containing basic helix−loop−helix motif (bHLH); these regulating proteins include, among others, neurogenin (Ngn) family, related to atonal family genes. Neurogenin 1 (NGN1) a 237-residue protein that contains bHLH domain and involved in neuronal differentiation. In this work, we synthesized region NGN1 (bHLHN) comprising residues 90−150 full-length NGN1. The monomeric natively unfolded with pH-dependent premolten...
Genetic screens are powerful methods for the discovery of gene-phenotype associations. However, a systems biology approach to genetics must leverage massive amount "omics" data enhance power and speed functional gene in vivo. Thus far, few computational function prediction have been rigorously tested their performance on genome-wide scale In this work, we demonstrate that integrating prioritization with large-scale genetic screening is tool discovery. To discover genes involved neural...
BBP proteins constitute a subclass of CUL3 interacting BTB whose in vivo function remains unknown. Here, we show that the Xenopus gene BTBD6 and single Drosophila homologue mammalian genes lute are strongly expressed developing nervous system. In Xenopus, expression responds positively to proneural negatively neurogenic overexpression. Knockdown or loss result embryos with strong defects late neuronal markers reduced disorganized axons while early neural development is unaffected. XBTBD6...
We previously mapped several quantitative trait loci (QTLs) controlling DMBA-induced mammary tumor development in female rats derived from a SPRD-Cu3 (susceptible strain) x WKY (resistant cross. Two of these QTLs were assigned to chromosomes 5 and 18. In the present study, we generated characterized congenic strains which segment or 18 was introduced genetic background, thereby physically demonstrating that each two controls multiplicity. The chromosome QTL (Mcstm1) accounts for 7 tumors per...
Abstract Wound response programs are often activated during neoplastic growth in tumors. In both wound repair and tumor growth, cells respond to acute stress balance the activation of multiple including apoptosis, proliferation, cell migration. Central those responses JNK/MAPK JAK/STAT signaling pathways. Yet, what extent these cascades interact at cis -regulatory level, how they orchestrate different regulatory phenotypic is still unclear. Here, we aim characterize states that emerge...
Abstract Single-cell technologies allow measuring chromatin accessibility and gene expression in each cell, but jointly utilizing both layers to map bona fide regulatory networks enhancers remains challenging. Here, we generate independent single-cell RNA-seq ATAC-seq atlases of the Drosophila eye-antennal disc spatially integrate data using a virtual latent space that mimics organization 2D tissue. To validate predicted enhancers, use large collection enhancer-reporter lines identify ∼85%...
The centromeric region of rat chromosome 2 (2q1) harbors unidentified quantitative trait loci genes that control tumor growth or development. To improve the mapping this region, we microdissected it and generated 10 new microsatellite markers, which included in linkage map and/or radiation hybrid 2q1, together with other known including four genes: <i>Pcsk1 </i>(protein convertase 1), <i>Dhfr</i> (dihydrofolate reductase), <i>Ndub13 </i>(NADH ubiquinone...
A stable narrow range of extracellular calcium concentration in the blood is essential for life. The calcium-sensing receptor (CaSR), a member G protein-coupled receptors family, required to adjust set point concentration, thus regulate parathyroid hormone (PTH) secretion and renal excretion. Loss or gain function CaSR mutations may result either hyper- hypocalcaemia. activating increase its sensitivity ionized (Ca2+). As consequence, PTH synthesis are suppressed continuously at normal...
Abstract The Neurogenin (Ngn) transcription factors control early neurogenesis and neurite outgrowth in mammalian cortex. In contrast to their proneural activity, function growth is poorly understood. Drosophila has a single predicted Ngn homologue called Tap, whose completely unknown. Here we show that Tap not protein but required for proper axonal guidance of neurons the mushroom body (MB), neuropile associative learning memory. Genetic expression analyses suggest inhibits excessive by...