A5100687826- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Extracellular vesicles in disease
- Fish Biology and Ecology Studies
- Fish Ecology and Management Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Circular RNAs in diseases
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Total Knee Arthroplasty Outcomes
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Developmental Biology and Gene Regulation
- Bone and Joint Diseases
- Aquaculture disease management and microbiota
- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Bone Metabolism and Diseases
- Plant Molecular Biology Research
- Knee injuries and reconstruction techniques
- Identification and Quantification in Food
Third Affiliated Hospital of Southern Medical University
2020-2025
Irvine Valley College
2024
Guangdong Province Stomatological Hospital
2023
Key Laboratory of Guangdong Province
2023
Guangzhou University
2023
VA Palo Alto Health Care System
2022
Stanford University
2022
Georgia State University
2022
Henan University of Science and Technology
2014-2021
Southern Medical University
2021
WNT5A is known to be involved in the pathogenesis of osteoarthritis. This study investigated molecular mechanism exosomal miR-92a-3p and chondrogenesis cartilage degeneration.Exosomal expression was assessed vitro a human mesenchymal stem cell (MSC) model normal OA primary chondrocytes (PHCs). MSCs PHCs were treated with exosomes derived from MSC-miR-92a-3p (MSC-miR-92a-3p-Exos) or its antisense inhibitor (MSC-anti-miR-92a-3p-Exos), respectively. Small interfering RNAs (siRNAs) luciferase...
Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression human chondrocytes. The aim this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p assessed mesenchymal stem cell (hMSC) model chondrogenesis, interleukin-1β (IL-1β)-treated primary chondrocytes (PHCs), non-degraded degraded cartilage. hMSCs PHCs were transfected with or its antisense...
Abstract The aim of the current study was to compare expression microRNAs (miRNAs) in exosomes derived from human bone mesenchymal stem cells (hBMSCs) with and without chondrogenic induction. Exosomes hBMSCs were isolated identified. Microarray analysis performed miRNA between induction, quantitative real‐time polymerase chain reaction (qRT‐PCR) used verify differentially expressed miRNAs. transfected mimic extract miRNA‐overexpressed exosomes. results showed that most exhibited a cup‐shaped...
Abstract Micro RNA s play critical roles in the pathogenesis of osteoarthritis, most common chronic degenerative joint disease. Exosomes derived from miR‐95‐5p‐overexpressing primary chondrocytes ( AC ‐miR‐95‐5p) may be effective treating osteoarthritis. Increased expression HDAC 2/8 occurs tissues and chondrocyte‐secreted exosomes patients with osteoarthritis mediates cartilage‐specific gene chondrocytes. We have been suggested that ‐miR‐95‐5p ‐miR‐95‐5p‐Exos) would enhance chondrogenesis...
Abstract MicroRNAs (miRNAs, miR) play a key role in the pathogenesis of osteoarthritis (OA). Few studies have examined regulatory P21-activated kinases (PAKs), family serine/threonine kinases, OA. The aim this study was to determine whether miR-455-3p can regulate cartilage degeneration OA by targeting PAK2. MiR-455-3p knockout mice showed significant knee cartilage. expression increased and PAK2 decreased late stage human adipose-derived stem cell (hADSC) chondrogenesis chondrocytes...
Increasing evidence suggest the biological roles of N6-methyladenosine (m6A) and long noncoding RNAs (lncRNAs) in bone disease, especially osteoarthritis (OA). However, interaction m6A lncRNA is still unclear. Here, we found that a m6A-related LINC00680 upregulated OA tissue IL-1β-induced isolated primary chondrocytes. Functionally, chondrocytes, silencing recovered proliferation repressed extracellular matrix (ECM) degradation. Mechanistically, methyltransferase METTL3 combined tithe site...
Chondrocyte senescence play an essential role in osteoarthritis (OA) progression. Recent studies have shown that snoRNA-derived RNA fragments (sdRNAs) are novel regulators of post-transcriptional gene expression. However, the expression profiles and their regulation chondrocyte OA progression is unknown. Here, we determined sdRNAs profile explored sdRNA-D43 its mechanism. We used qPCR arrays to determine chondrocytes areas undamaged damaged three knee samples. SdRNA-D43 was using...
Long non-coding RNAs (lncRNAs) play pivotal roles in diseases such as osteoarthritis (OA). However, knowledge of the biological lncRNAs is limited OA. We aimed to explore function and molecular mechanism HOTTIP chondrogenesis cartilage degradation. used human mesenchymal stem cell (hMSC) model chondrogenesis, parallel with, tissue biopsies from normal OA detect HOTTIP, CCL3, miR-455-3p expression vitro. Biological interactions between were determined by RNA silencing overexpression evaluated...
The neurogenin (Ngn) transcription factors control early neurogenesis and neurite outgrowth in mammalian cortex. In contrast to their proneural activity, function growth is poorly understood. Drosophila has a single predicted Ngn homolog, Tap, of unknown function. Here we show that Tap not protein but required for proper axonal guidance neurons the mushroom body, neuropile associative learning memory. Genetic expression analyses suggest inhibits excessive by fine regulation levels Wnt...
Abstract The effect of residual varus on survival rate and function in patients with knee osteoarthritis (OA) was considered an important issue for successful primary total arthroplasty (TKA). In this study, we compared the midterm clinical functional outcomes different varus. A retrospective review 175 (219 knees) OA > 3° hip-knee-ankle (HKA) who underwent TKA after exclusions loss to follow-up from 237 (281 knees). mean period 5.2 ( ± 1.1) years. Patients were divided into four groups...
Knee osteoarthritis (KOA) is a highly prevalent disabling joint disease in aged people. Progressive cartilage degradation the hallmark of KOA, but its deeper mechanism remains unclear. Substantial evidence indicates importance synovium for homeostasis. The present study aimed to determine whether regulates metabolism through chondrogenesis-related microRNAs (miRNAs) KOA microenvironment. Clinical sample testing and vitro cell experiments screened out miR-455 miR-210 as effective miRNAs....
Meniscus plays an important role in joint homeostasis. Tear or degeneration of meniscus might facilitate the process knee osteoarthritis (OA). Hence, to investigate transcriptome change during degeneration, we reveal alterations messenger RNA (mRNA), microRNA (miRNA), long noncoding (lncRNA), and circular (circRNA) OA by whole-transcriptome sequence. A total 375 mRNAs, 15 miRNAs, 56 lncRNAs, 90 circRNAs were significantly altered degenerative treated with interleukin-1β (IL-1β). More...
MicroRNAs (miRNAs) play a pivotal role in cartilage development and homeostasis osteoarthritis (OA). While the fundamental roles of miRNAs degeneration have been extensively studied, their effects on chondrogenic differentiation induced by human adipose-derived stem cells (hADSCs) underlying mechanisms remain largely elusive. Here, we investigated hADSC chondrocyte homeostasis. Using microarray analysis, screened expressed differentiated hADSCs identified miR-490-5p as most significantly...
Chronic venous insufficiency (CVI) is the consequence of valve reflux and/or flow obstruction and resulting hypertension in lower extremities. The aim this prospective supplement registry study was to evaluate efficacy compression stockings or Pycnogenol
Genetic screens are powerful methods for the discovery of gene-phenotype associations. However, a systems biology approach to genetics must leverage massive amount "omics" data enhance power and speed functional gene in vivo. Thus far, few computational function prediction have been rigorously tested their performance on genome-wide scale In this work, we demonstrate that integrating prioritization with large-scale genetic screening is tool discovery. To discover genes involved neural...
Abstract The Zig-zag eel ( Mastacembelus armatus ) is an economically important species in southern China. Its natural resources have declined year by due to overfishing. Understanding its genetic diversity and population structure very for resource conservations. Here, we first successfully developed 28 polymorphic microsatellite markers zig-zag eels ten of them were used examine the differentiation 7 populations collected from major river systems south In total, 224 alleles found with 10...
Osteoarthritis (OA) is a degenerative disease causing the progressive destruction of articular cartilage; however, aetiology has not yet been elucidated. Circular RNAs (circRNAs) are reportedly involved in cartilage degeneration and OA development. In present study, we identified that circNFIX regulates chondrogenesis homeostasis.Microarray analysis was performed to explore circRNA expression during chondrogenic differentiation human adipose-drived stem cells (hADSCs). CircNFIX determined...
Osteoarthritis (OA), which involves total joint damage and dysfunction, is a leading cause of disability worldwide. However, its exact pathogenesis remains unclear. Here, we identified TCF12 as an important regulator the progression OA. qRT-PCR, immunoblotting immunohistochemistry (IHC) were used to detect expression level TCF12. The interaction with downstream factor CXCR4 was assessed by Western blotting, immunofluorescence, qRT-PCR luciferase assays. A mouse model generated examine...
Long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). However, biological functions lncRNA small ubiquitin-like modifier 1 pseudogene 3 (SUMO1P3) and underlying mechanisms remain unclear. In this study, we revealed that SUMO1P3 expression was enhanced HCC tissues cell lines, positively associating with tumor size number, poor differentiation, lymphatic distant metastasis, TNM stage, prognosis patients. vitro assays showed...