A5039637599- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Inflammatory mediators and NSAID effects
- Bone Metabolism and Diseases
- Mass Spectrometry Techniques and Applications
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Additive Manufacturing Materials and Processes
- TGF-β signaling in diseases
- Bone and Joint Diseases
- Cell Adhesion Molecules Research
- Total Knee Arthroplasty Outcomes
- NF-κB Signaling Pathways
- PI3K/AKT/mTOR signaling in cancer
- Knee injuries and reconstruction techniques
- Bone Tissue Engineering Materials
- Toxin Mechanisms and Immunotoxins
- Cytokine Signaling Pathways and Interactions
- Rheumatoid Arthritis Research and Therapies
- Liver Disease Diagnosis and Treatment
- Galectins and Cancer Biology
- Cancer Research and Treatments
Third Affiliated Hospital of Southern Medical University
2016-2025
Southern Medical University
2021-2024
Key Laboratory of Guangdong Province
2020-2024
Guangdong Province Stomatological Hospital
2018-2024
Lishui Central Hospital
2021-2024
Wenzhou Medical University
2024
Quzhou City People's Hospital
2024
Weatherford College
2023
Anhui University
2023
Zhejiang University
2021-2022
To examine the early changes of articular cartilage and subchondral bone in DMM mouse model osteoarthritis, mice were subjected to or SHAM surgery sacrificed at 2-, 5- 10-week post-surgery. Catwalk gait analyses, Micro-Computed Tomography, Toluidine Blue, Picrosirius Red Tartrate-Resistant Acid Phosphatase (TRAP) staining used investigate patterns, joint morphology, bone, cartilage, collagen organization osteoclasts activity, respectively. Results showed OA progressed over time-course. Gait...
Fibronectin (FN)-mediated cell adhesion is controlled mainly by alpha 5 beta 1 (recognizing the RGD sequence) and 4 CS-1 peptide sequence of FN) integrin receptors. Integrin-dependent to FN greatly promoted optimal GM3 concentration at surface membrane (Zheng, M., Fang, H., Tsuruoka, T., Tsuji, Sasaki, Hakomori, S. (1993) J. Biol. Chem. 268, 2217-2222), mediated (to or 6 laminin) inhibited modifying N-glycosylation processing receptor (e.g. Akiyama, K., Yamada, S., K. M. (1989) 264,...
Increasing evidences show that aberrant subchondral bone remodeling plays an important role in the development of osteoarthritis (OA). However, how formation is activated and mechanism by which increased turnover promotes cartilage degeneration during OA remains unclear. Here, we mechanistic target rapamycin complex 1 (mTORC1) pathway preosteoblasts (Osterix+) from patients mice. Constitutive activation mTORC1 deletion upstream inhibitor, tuberous sclerosis 1, induced formation, with...
Mouse mammary carcinoma mutant cell line FUA169, characterized by high GM3 ganglioside content, was established from parent FM3A/F28-7, which has lactosyl ceramide (LacCer) content but no GM3. FUA169 displays changes in protein glycosylation, and is a typical glycolipid differing its that it contains quantities of GlcCer, LacCer (see accompanying paper; Tsuruoka, T., Tsuji, Nojiri, H., Holmes, E. Hakomori, S. (1993) J. Biol. Chem. 268, 2211-2216). In contrast to F28-7 cells, cells showed...
PI3K/AKT signaling is essential in regulating pathophysiology of osteoarthritis (OA). However, its potential modulatory role early OA progression has not been investigated yet. Here, a mouse destabilization model the tibia was used to investigate roles subchondral bone changes and pathological process. We revealed significant increase activation which associated with aberrant formation tibial following destabilizing medial meniscus (DMM), effectively prevented by treatment inhibitor...
Vascular-invasion-mediated interactions between activated articular chondrocytes and subchondral bone are essential for osteoarthritis (OA) development. Here, we determined the role of nutrient sensing mechanistic target rapamycin complex 1 (mTORC1) signaling in crosstalk across cartilage interface its regulatory mechanisms. Then mice with chondrocyte-specific mTORC1 activation (Tsc1 CKO Tsc1 CKOER ) or inhibition (Raptor their littermate controls were subjected to OA induced by...
Treatment of metastatic, castration-resistant prostate cancer (mCRPC) remains a highly unmet medical need and current therapies ultimately result in disease progression. Immunotherapy is rapidly growing approach for treatment but has shown limited success to date the mCRPC. We have developed novel humanized bispecific antibody, MOR209/ES414, built on ADAPTIR (modular protein technology) platform, redirect T-cell cytotoxicity toward cells by specifically targeting T through CD3ε expressing...
Osteoarthritis (OA) is a degenerative joint disease that affects both cartilage and bone. A better understanding of the early molecular changes in subchondral bone may help elucidate pathogenesis OA. We used microarray technology to investigate time course stages experimental osteoarthritis rat model. identified 2,234 differentially expressed (DE) genes at 1 week, 1,944 2 weeks 1,517 4 post-surgery. Further analyses dysregulated indicated events underlying remodeling occurred sequentially...
The role of mechanical stress and transforming growth factor beta 1 (TGF-β1) is important in the initiation progression osteoarthritis (OA). However, underlying molecular mechanisms are not clearly known.In this study, TGF-β1 from osteoclasts knee joints were analyzed using a co-cultured cell model an OA rat model, respectively. Five patients with femoral neck fracture (four female one male, mean 73.4 years (68 to 79)) recruited between January 2015 December 2015. Results showed that was...
Deploying Large Language Models (LLMs) on edge devices is increasingly important, as it eliminates reliance network connections, reduces expensive API calls, and enhances user privacy. However, on-device deployment challenging due to the limited computational resources of devices. In particular, key bottleneck stems from memory bandwidth constraints related weight loading. Weight-only quantization effectively access, yet often induces significant accuracy degradation. Recent efforts...
Osteoarthritis (OA) is an aging-related chronic degenerative disease characterized by the degradation of chondrocyte extracellular matrix (ECM).Previous studies have suggested that microRNAs (miRNAs) are associated with OA, but role miR-146b in OA remains unclear.The aim this study was to determine progression.The effect on ECM were studied mouse chondrocytes transfected miRNA and treated IL-1β.Cell viability expression levels proteolytic enzymes cells assessed real-time RT-PCR, ELISA...
In this study, we using the in vivo destabilization of medial meniscus (DMM) mouse model to investigate role bone morphogenetic protein 5 (BMP5) osteoarthritis (OA) progression mediated via chondrocyte senescence and apoptosis. BMP5 expression was significantly higher knee articular cartilage tissues OA patients DMM mice than corresponding controls. The Osteoarthritis Research Society International scores based on histological staining sections were lower where knocked down chondrocytes...
Osteoarthritis (OA) is a chronic degenerative joint disorder. Previous studies have shown abnormally increased apoptosis of chondrocytes in patients and animal models OA. TNF-α nitric oxide been reported to induce chondrocyte ageing; however, the mechanism induced by IL-1β has remained unclear. The aim this study identify role c-Jun N-terminal kinase (JNK) - pathway regulating induction Bim, its implication apoptosis. This showed that Bim upregulated obtained from articular cartilage OA...
Abstract Objectives This study aimed to investigate the role and mechanism of asporin in modulating chondrocyte senescence OA pathology. Methods Asporin senescence-related hallmark expression were examined human experimental mouse cartilage samples. Twelve-week-old male C57 mice administered with recombinant protein (rm-asporin)- or asporin-siRNA-expressing lentiviruses via intra-articular injection once a week after destabilization medial meniscus (DMM) surgery induce OA. Cartilage damage...
Osteoarthritis (OA) is the most common disease of joints, and characterized by breakdown cartilage degradation extracellular matrix. OA causes a high level patient suffering incurs large societal costs; however, current strategies for treating are restricted due to limited understanding underlying molecular cellular mechanisms. In present study, beneficial effects isoimperatorin (Iso) were investigated using an experimental mouse model OA, its mechanism action on primary chondrocytes was...
The technique of selective laser melting (SLM) has been widely applied as an additive manufacturing method in fabricating Ti6Al4V parts. However, the inert surface SLM titanium alloy restricts its biomedical application especially bone implants material. In this work, micro-arc oxidation (MAO) was proposed to improve biological performance by forming a ceramic coating containing calcium (Ca) and phosphorus (P) on surface. influence polarization voltage, which ranged from 200 400 V,...
Myostatin, a member of the transforming growth factor-β family, contributes to joint deterioration in mice. Thus, we aimed assess correlation myostatin concentrations with presence and severity knee osteoarthritis (OA).We determined serum synovial fluid (SF) population 184 patients OA 109 healthy controls.The group presented higher than controls. Knee KL grade 4 showed SF compared those 2 3. 3 had 2. Serum were significantly correlated grading.Serum OA.