A5074705666- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Endoplasmic Reticulum Stress and Disease
- Telomeres, Telomerase, and Senescence
- Iron Metabolism and Disorders
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Trace Elements in Health
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Nanoplatforms for cancer theranostics
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- RNA modifications and cancer
- Extracellular vesicles in disease
- Chromosomal and Genetic Variations
- Acute Myeloid Leukemia Research
- Biotin and Related Studies
- Transgenic Plants and Applications
Institute of Molecular and Cell Biology
2017-2024
National University of Singapore
2005-2024
Agency for Science, Technology and Research
2017-2024
National Neuroscience Institute
2021-2024
GlobalFoundries (Germany)
2024
The University of Texas MD Anderson Cancer Center
2012-2017
Centre for Cancer Biology
2010-2015
Mississippi Delta Community College
2014
Scripps Research Institute
2008-2013
Chungnam National University
2010
Cancer cells adopt metabolic reprogramming to promote cell survival under stress. A key regulator of metabolism is AMP-activated protein kinase (AMPK) which promotes catabolism while suppresses anabolism. However, the underlying mechanism AMPK in handling stress cancer remains be fully understood. In this study, by performing a proteomics screening AMPK-interacting proteins non-small-cell lung (NSCLC) cells, we discovered platelet isoform phosphofructokinase 1 (PFKP), rate-limiting enzyme...
An integrated genomic and functional analysis to elucidate DNA damage signaling factors promoting self-renewal of glioma stem cells (GSCs) identified proliferating cell nuclear antigen (PCNA)-associated factor (PAF) up-regulation in glioblastoma. PAF is preferentially overexpressed GSCs. Its depletion impairs maintenance without differentiation reduces tumor-initiating frequency. Combined transcriptomic metabolomic analyses revealed that supports GSC maintenance, part, by influencing...
Abstract The histone variant H2AZ is overexpressed in diverse cancer types where it facilitates the accessibility of transcriptional regulators to promoters cell cycle genes. However, molecular basis for its dysregulation remains unknown. Here, we report that glioblastomas (GBM) and glioma stem cells (GSCs) preferentially overexpress their proliferation, stemness tumorigenicity. Chromatin analysis H2AZ2 depleted GSC revealed E2F1 occupies enhancer region within gene promoter, thereby...
Abstract Epitranscriptomic RNA modifications are crucial for the maintenance of glioma stem cells (GSCs), most malignant in glioblastoma (GBM). 3-methylcytosine (m 3 C) is a new epitranscriptomic mark on RNAs and METTL8 represents an m C writer that dysregulated cancer. Although has established function mitochondrial tRNA (mt-tRNA) modification, alternative splicing can also generate isoforms localize to nucleolus where they may regulate R-loop formation. The molecular basis dysregulation...
Plasma ferritin is an important extracellular iron storage molecule, whose concentration increases drastically in cancer and infection. During infection, the pathogen usurps host for its survival pathogenicity; hence, maintenance of plasma level during infection a crucial defence mechanism. In this study, horseshoe crab complex was purified, characterized, involvement innate immune investigated. The appears as 21-kDa subunit on SDS-PAGE. Full-length ferritin-H cDNAs (CrFer-H1 CrFer-H2) were...
SETDB1 is a key regulator of lineage-specific genes and endogenous retroviral elements (ERVs) through its deposition repressive H3K9me3 mark. Apart from regulatory role, has seldom been studied in terms other potential roles. To investigate this, genomic survey binding mouse embryonic stem cells across multiple libraries was conducted, leading to the unexpected discovery regions bereft common histone marks (H3K9me3, H3K27me3). These were enriched with CTCF motif that often associated...
Plasma ferritin is an important extracellular iron storage molecule, whose concentration increases drastically in cancer and infection. During infection, the pathogen usurps host for its survival pathogenicity; hence, maintenance of plasma level during infection a crucial defence mechanism. In this study, horseshoe crab complex was purified, characterized, involvement innate immune investigated. The appears as 21-kDa subunit on SDS-PAGE. Full-length ferritin-H cDNAs (CrFer-H1 CrFer-H2) were...
We describe a non-fluorescent, second generation stilbene that very selectively binds to transthyretin in complex biological environments and remains dark until it chemoselectively reacts with the pKa-perturbed Lys-15 ε-amino group of form bright blue fluorescent conjugate. Stilbene A2 is mechanistically unusual non-fluorescent cell lysates lacking transthyretin, even though there likely some proteome binding. Thus, especially useful for cellular imaging, as background fluorescence...
Abstract Cohesin plays a crucial role in the organization of topologically-associated domains (TADs), which influence gene expression and DNA replication timing. Whether epigenetic regulators may affect TADs via cohesin to mediate remains elusive. Here, we discover that histone demethylase PHF2 associates with RAD21, core subunit cohesin, regulate mouse neural stem cells (NSC). loss impairs due activation dormant origins NSC. Notably, PHF2/RAD21 co-bound genomic regions are characterized by...
Nanoparticles have been explored in glioblastomas as they can traverse the blood-brain barrier and target glioblastoma selectively. However, direct observation of nanoparticle trafficking into cells their underlying intracellular fate after systemic administration remains uncharacterized. Here, based on high-resolution transmission electron microscopy experiments an intracranial model, it is shown that ligand-modified nanoparticles barrier, endocytose lysosomes cells, undergo endolysosomal...
Glioblastoma (GBM) is a uniformly lethal disease driven by glioma stem cells (GSCs). Here, we use chemical biology approach to unveil previously unknown GBM dependencies. By studying sulconazole (SN) with anti-GSC properties, find that SN disrupts biotin distribution the carboxylases and histones. Transcriptomic metabolomic analyses of SN-treated GSCs reveal metabolic alterations are characteristic biotin-deficient cells, including intracellular cholesterol depletion, impairment oxidative...
Abstract Telomere shortening is a well-established hallmark of cellular aging. Telomerase reverse transcriptase (TERT) plays crucial role in maintaining the length telomeres, which are specialised protective caps at end chromosomes. The lack vitro aging models, particularly for central nervous system (CNS), has impeded progress understanding and age-associated neurodegenerative diseases. In this study, we aimed to explore possibility inducing aging-associated features cell types CNS using...
Abstract MAD2 is a spindle assembly checkpoint protein that participates in the formation of mitotic complex, which blocks progression. RNF8, an established DNA damage response protein, has been implicated regulation but its exact role remains poorly understood. Here, RNF8 proximity proteomics uncovered RNF8-MAD2 generating signal. Specifically, competes with small pool p31 comet for binding to closed conformer via RING domain, while CAMK2D serves as molecular scaffold concentrate complex...
Abstract The relatively quiet mutational landscape of rhabdomyosarcoma (RMS) suggests that epigenetic deregulation could be central to oncogenesis and tumour aggressiveness. Histone variants have long been recognised as important regulators gene expression. However, the role histone in RMS has not studied hitherto. In this study, we show variant H3.3 is overexpressed alveolar (ARMS), an aggressive subtype RMS. Functionally, knockdown H3F3A , which encodes for H3.3, significantly impairs...