A5071088268- Protease and Inhibitor Mechanisms
- Liver Disease Diagnosis and Treatment
- Lysosomal Storage Disorders Research
- Polysaccharides and Plant Cell Walls
- Toxin Mechanisms and Immunotoxins
- Retinoids in leukemia and cellular processes
- Studies on Chitinases and Chitosanases
- Drug-Induced Hepatotoxicity and Protection
- Biomedical Research and Pathophysiology
- Autophagy in Disease and Therapy
- Pancreatitis Pathology and Treatment
- Cancer, Lipids, and Metabolism
- Folate and B Vitamins Research
- Peptidase Inhibition and Analysis
- Drug Transport and Resistance Mechanisms
- Bone health and treatments
- Tryptophan and brain disorders
- Cell death mechanisms and regulation
- Lipoproteins and Cardiovascular Health
- Advanced Glycation End Products research
- Research on Leishmaniasis Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Parkinson's Disease Mechanisms and Treatments
- Lipid metabolism and disorders
- Alzheimer's disease research and treatments
Scientific Research Institute of Biochemistry
2021-2023
Institute of Physiology and Basic Medicine
1997-2020
Research Institute of Physiology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Medical Sciences
2000-2019
Federal Agency for Scientific Organizations
2016-2017
University Canada West
2016
Russian Academy of Sciences
1992-2015
Czech Academy of Sciences, Institute of Physiology
1992-2015
Academy of Medical Sciences
1992-2014
Novosibirsk State Medical University
1975-2007
Institute of Cytology and Genetics
2001-2004
Autophagy attenuation has been found in neurodegenerative diseases, aging, diabetes mellitus, and atherosclerosis. In experimental models of the correction autophagy brain reverses neuronal behavioral deficits hence seems to be a promising therapy for neuropathologies. Our aim was study effect an inducer, trehalose, on behavior genetic model with signs damage (db/db mice). A 2% trehalose solution administered as drinking water during 24 days experiment. Expressions markers (LC3-II),...
In the treatment of experimental neurodegeneration with disaccharide trehalose, various regimens are used, predominantly a 2% solution, drunk for several weeks. We studied effects different dietary trehalose in an amyloid-β (Aβ) 25–35-induced murine model Alzheimer’s disease (AD). Aβ-treated mice received solution daily, 4% daily (continuous mode) or every other day (intermittent mode), to drink two revealed dose-dependent on autophagy activation frontal cortex and hippocampus, restoration...
The aims of this study were to evaluate the effect poloxamer 407 administration on atherogenic serum lipoprotein fractions and subfractions associated with cholesterol, triglycerides phospholipids, as well onset early atherosclerosis, in mice.Mice administered either sterile saline or (to induce a dose-controlled hyperlipidemia) for 1 month then sacrificed at 1, 4 10 days after last dose 407. Systolic diastolic blood pressure, activity cysteine protease (cathepsin B) cardiac liver tissue,...
Huntington's disease (HD) is a progressive and fatal neurodegenerative caused by CAG repeat expansion in the coding region of huntingtin (HTT) protein. The accumulation mutant HTT (mHTT) contributes to neurotoxicity causing autophagy defects oxidative stress that ultimately lead neuronal death. Interestingly, epidemiologic studies have demonstrated prevalence type-2 diabetes, metabolic mainly defective insulin signaling, higher patients with HD than healthy controls. Although precise...
ObjectivesTo investigate the possible role of cystatin C in eye biological fluids locally and serum lactoferrin revealing anti-tumor activity tumor development.BackgroundThe increased number tumors was registered recently not only countries with high insolation, but also northern including Russia (11 cases per million population). Search for new markers is important diagnosis prognosis tumors. Cystatin C, an endogenous inhibitor cysteine proteases, plays protective several Lactoferrin shown...
ObjectivesTo evaluate procathepsin B, as well endogenous inhibitors of cysteine proteases (cystatin B and cystatin C) in biological fluids possible biomarkers ovarian cancer. To observe levels serum different age groups.Study designThe sample (N=27) women with gynaecological tumours included 18 patients cancer (n=18) 9 benign (n=9); 72 healthy were the control group. All treated Novosibirsk Regional Oncological Center, Russia. Serum samples (n=40) aged 18–70 years used controls for common...
Db/db mice (carrying a mutation in the gene encoding leptin receptor) show autophagy suppression. Our aim was to evaluate effect of inducer trehalose on liver and heart db/db study inflammation dysregulation suitability chitinases' expression levels as diabetes markers. Thirty-eight male C57/BL (control) were used. The model manifested symptoms: overexpression TNF-α spleen underexpression IL-10 (cytokine imbalance). Simultaneously, we revealed decreased chitotriosidase (CHIT1) acid mammalian...
The effects of atorvastatin and carboxymethylated β-glucan (CMG) on the lipoprotein-cholesterol (LP-C) lipoprotein-triglyceride (LP-TG) fractions subfractions at early stage murine hyperlipidemia, its pleiotropic anti-inflammatory effects, were studied. Atorvastatin CMG administered in ICR male mice with acute lipemia induced a single injection poloxamer 407 (P-407). A novel small-angle X-ray scattering method for determination fractional subfractional composition LP-C LP-TG was used. In...
The effects of repeated administration poloxamer 407 (P-407) on lipoprotein-cholesterol (LP-C) and lipoprotein-triglyceride (LP-TG) fractions subfractions, as well the effect liver heart proteases, were studied. Repeated P-407 to male CBA mice resulted in a model atherosclerosis with increased diastolic blood pressure; there was drastic increase total serum cholesterol especially TG. A novel small-angle X-ray scattering method for determination fractional subfractional composition LP-C LP-TG...
Chronic administration of the poloxamer 407 (P-407), a block copolymer, to elevate serum lipids in mice is well-established mouse model hyperlipidemia and atherosclerosis. We tested hypothesis that activity several types proteases heart liver tissue changed early stages atherosclerosis development. Additionally, we evaluated whether increased would induce anxiety mice, as determined by using 'plus-maze' test. The were administered P-407 intraperitoneal injection twice week for one month....