A5077060561- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Hip and Femur Fractures
- Hydrogen's biological and therapeutic effects
- Cancer-related Molecular Pathways
- Circular RNAs in diseases
- BRCA gene mutations in cancer
- Telomeres, Telomerase, and Senescence
- Animal Genetics and Reproduction
- Fish biology, ecology, and behavior
- Cancer-related molecular mechanisms research
- Frailty in Older Adults
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Chronic Disease Management Strategies
Albert Einstein College of Medicine
2019-2024
Montefiore Medical Center
2024
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2024
Goethe University Frankfurt
2017-2019
Abstract The short-lived turquoise killifish Nothobranchius furzeri (Nfu) is a valid model for aging studies. Here, we investigated its age-associated cardiac function. We observed oxidative stress accumulation and an engagement of microRNAs (miRNAs) in the heart. MiRNA-sequencing 5 week (young), 12–21 (adult) 28–40 (old) Nfu hearts revealed 23 up-regulated 18 down-regulated miRNAs with age. MiR-29 family turned out as one most during aging. increase induces decrease known targets like...
Postzygotic somatic mutations have been found associated with human disease, including diseases other than cancer. Most information on has come from studying clonally amplified mutant cells, based a growth advantage or genetic drift. However, almost all are unique for each cell, and the quantitative analysis of these low-abundance in normal tissues remains major challenge biology. Here, we introduce single-molecule mutation sequencing (SMM-seq), novel approach identification point cells tissues.
Abstract At over 200 years, the maximum lifespan of bowhead whale exceeds that all other mammals. The is also second-largest animal on Earth, reaching 80,000 kg 1 . Despite its very large number cells and long lifespan, not highly cancer-prone, an incongruity termed Peto’s Paradox 2 This phenomenon has been explained by evolution additional tumor suppressor genes in larger animals, supported research elephants demonstrating expansion p53 gene 3–5 Here we show fibroblasts undergo oncogenic...
Aging associates with progressive loss of skeletal muscle function, sometimes leading to sarcopenia, a process characterized by impaired mobility and weakening strength. Since aging profound epigenetic changes, landscape alteration analysis in the promises highlight molecular mechanisms age-associated muscle. This study was conducted exploiting short-lived turquoise killifish Nothobranchius furzeri (Nfu), relatively new model for studies. The suggested less accessible more condensed...
Aging associates with progressive loss of skeletal muscle function leading up to sarcopenia, a process characterized by impaired mobility and weakening strength. Molecular mechanisms underpinning sarcopenia are still poorly characterized. Since aging profound epigenetic changes, landscape alteration analysis in the promises highlight molecular age-associated sarcopenia. The study was conducted exploiting short-lived turquoise killifish Nothobranchius furzeri (Nfu), relatively new model for...
Single-cell sequencing has shown that thousands of mutations accumulate with age in most human tissues. While there is ample evidence some can clonally amplify and lead to disease, the total burden a cell tolerates without functional decline remains unknown. Here we addressed this question by exposing primary fibroblasts multiple, low doses N-ethyl-N-nitrosourea (ENU) analyzed somatic mutation using single-cell whole genome sequencing. The results indicate individual cells sustain ~60,000...
The purpose of this study was to (1) perform the first analysis bone-derived DNA methylation, (2) compare methylation clocks derived from bone with those whole blood, and (3) establish a relationship between age 1-year mortality within geriatric hip fracture population.
Abstract Detecting somatic mutations in normal cells and tissues is notoriously challenging due to their low abundance, orders of magnitude below the sequencing error rate. While several techniques, such as single-cell single-molecule sequencing, have been developed identify mutations, they are insufficient for detecting genomic structural variants (SVs), which a significantly greater impact than single-nucleotide (SNVs). We introduce Single-Molecule Mutation Sequencing Structural Variants...
Thus far, multiple techniques for single cell analysis have been developed, yet we lack a relatively simple tool to assess DNA and RNA from the same at whole-transcriptome whole-genome depths. Here present an updated method physical separation of cytoplasmic nuclei, which allows simultaneous studies cell. The consists three steps-(1) immobilization on solid substrate, (2) hypotonic lysis immobilized cell, (3) cytosol containing aqueous phase nucleus. We found that extracted using our...
Thus far, multiple techniques for single cell analysis have been developed, yet we lack a relatively simple tool to assess DNA and RNA from the same at whole-transcriptome whole-genome depths. Here present an updated method physical separation of cytoplasmic nuclei, which allows simultaneous studies cell. The consists three steps - 1) immobilization on solid substrate, 2) hypotonic lysis immobilized cell, 3) cytosol containing aqueous phase nucleus. We found that extracted using our approach...